Rat MMP-2 ELISA Kit PicoKine™

Rat MMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 20 publication(s).

Product Info Summary

SKU: EK0639
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Rat MMP-2 ELISA Kit PicoKine™

See all MMP-2 products

SKU/Catalog Number

EK0639

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat MMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat MMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0639)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-C662

Sensitivity

<10 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0639 is reactive to Mmp2 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0639 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0639-CAP Anti-Rat Mmp2 Pre-coated 96-well strip microplate 1
EK0639-ST Rat Mmp2 Standard 2 vials, 40 ng/tube
EK0639-DA Rat Mmp2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.1310.1680.2130.3320.5850.9251.4982.082

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat MMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10934492230731098444021240
Standard deviation68.85296.471107.590.03368.521296.64
CV (%)8.3%6.6%4.8%8.2%8.3%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1109310909751117106855.125.1%
Sample 244923849440244954309268.486.2%
Sample 323073231132291926456238901483.16.2%
*number of samples for each test n=16.

Gene/Protein Information For Mmp2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P33436

Gene ID

81686

Gene Name

Mmp2

Full Name

72 kDa type IV collagenase

Weight

74.149kDa

Superfamily

peptidase M10A family

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) Mmp2|matrix metallopeptidase 2|72 kDa type IV collagenase|72 kDa gelatinase|MMP-2|gelatinase A|matrix metalloproteinase-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mmp2, check out the Mmp2 Infographic

Mmp2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0639 has been cited in 20 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Bulboacă AE,Porfire A,Bolboacă SD,Nicula CA,Feștilă DG,Roman A,Râjnoveanu RM,Râjnoveanu A,Dogaru G,Boarescu PM,Rus V, Bulboacă CA,Bulboacă AI,Stănescu I.Protective Effects of Liposomal Curcumin on Oxidative Stress/Antioxidant Imbalance, Metalloproteinases
Species: Rat
EK0639 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Usunier,B.;Brossard,C.;L‘Homme,B.;Linard,C.;Benderitter,M.;Milliat,F.;Chapel,A.HGF and TSG-6 Released by Mesenchymal Stem Cells Attenuate Colon Radiation-Induced Fibrosis.Int.J.Mol.Sci.2021,22,1790.https://doi.org/10.3390/ijms22041790
Species: Human,Rat
EK0639 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

KATP channels in high glucose-induced rat mesangial cell proliferation and release of MMP-2 and fibronectin

A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats

Role of Sphingosine Kinase/S1P Axis in ECM Remodeling of Cardiac Cells Elicited by Relaxin

You C, Zu J, Liu X, Kong P, Song C, Wei R, Zhou C, Wang Y, Yan J. J Cell Mol Med. 2018 Apr;22(4):2449-2457. doi: 10.1111/jcmm.13549. Epub 2018 Jan 30. Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymo...

Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...

Zhang L, Li Y, Liu Y, Wang X, Chen M, Xing Y, Zhu D. J Steroid Biochem Mol Biol. 2015 May;149:106-17. Doi: 10.1016/J.Jsbmb.2015.01.015. Epub 2015 Jan 23. Stat3-Mediated Mmp-2 Expression Is Required For 15-Hete-Induced Vascular Adventitial Fibrobla...

Qin Y, Liu Xj, Li L, Liu Xj, Li Y, Gao Rj, Shao Rg, Zhen Ys. Oncol Rep. 2014 Jul;32(1):121-30. Doi: 10.3892/Or.2014.3169. Epub 2014 May 7. Mmp-2/9-Oriented Combinations Enhance Antitumor Efficacy Of Egfr/Her2-Targeting Fusion Proteins And Gemcitab...

Simmers P, Gishto A, Vyavahare N, Kothapalli Cr. Tissue Eng Part A. 2015 Apr;21(7-8):1455-70. Doi: 10.1089/Ten.Tea.2014.0363. Epub 2015 Mar 9. Nitric Oxide Stimulates Matrix Synthesis And Deposition By Adult Human Aortic Smooth Muscle Cells Within...

Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...

Tang Xp, Tang Gd, Fang Cy, Liang Zh, Zhang Ly. World J Gastroenterol. 2013 Mar 14;19(10):1582-92. Doi: 10.3748/Wjg.V19.I10.1582. Effects Of Ginsenoside Rh2 On Growth And Migration Of Pancreatic Cancer Cells.

Yuan H, Yang P, Zhou D, Gao W, Qiu Z, Fang F, Ding S, Xiao W. Mol Biol Rep. 2014 Aug;41(8):5157-65. Doi: 10.1007/S11033-014-3382-4. Epub 2014 May 10. Knockdown Of Sphingosine Kinase 1 Inhibits The Migration And Invasion Of Human Rheumatoid Arthrit...

Mazani M, Fard As, Baghi An, Nemati A, Mogadam Ra. J Pak Med Assoc. 2014 Jul;64(7):785-90. Effect Of Pomegranate Juice Supplementation On Matrix Metalloproteinases 2 And 9 Following Exhaustive Exercise In Young Healthy Males.

Liu Q,, Zou R, Zhou R, Gong C, Wang Z, Cai T, Tan C, Fang J. J Cell Biochem. 2015 Jul;116(7):1213-21. Doi: 10.1002/Jcb.25073. Mir-155 Regulates Glioma Cells Invasion And Chemosensitivity By P38 Isforms In Vitro.

Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...

Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.

Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.

Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.

Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

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10 Customer Q&As for Rat MMP-2 ELISA Kit PicoKine™

Question

Q: how do I analyze ELISA data? I have obtained MMP-2 level in serum.

A. Wang

Verified customer

Asked: 2019-05-27

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-05-27

Question

Q: can I use EDTA plasma as samples in Rat MMP-2 Picokine® ELISA Kit (Catalog # EK0639)?

Verified Customer

Verified customer

Asked: 2019-05-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of MMP-2 causing degradation of its protein structure. MMP-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-2 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-11

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MMP-2 using the EK0639 Rat MMP-2 Picokine® ELISA Kit?

G. Anderson

Verified customer

Asked: 2018-12-14

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-12-14

Question

Q: which procedure should I follow in order to thaw whole blood sample for MMP-2 ELISA after freezing?

W. Tiwari

Verified customer

Asked: 2018-12-11

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-12-11

Question

Q: can you recommend the dilution ratio of serum samples for detection of MMP-2 in Rat serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-07-01

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-07-01

Question

Q: Can MMP-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

S. Banerjee

Verified customer

Asked: 2018-05-17

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-05-17

Question

Q: What is the optimal O.D. value for MMP-2 ELISA kit? I used your MMP-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MMP-2 even though the O.D. values are not very high?

M. Johnson

Verified customer

Asked: 2018-04-19

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-04-19

Question

Q: if the enzyme conjugated MMP-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-2 antigen?

Verified Customer

Verified customer

Asked: 2016-11-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-11-14

Question

Does EK0639 detect active MMP-2 or the full MMP-2 rat protein? Will it cross-react with other proteins?

Verified Customer

Verified customer

Asked: 2016-09-12

Answer

EK0639 is able to detect the full MMP-2 rat protein including zymogen and active MMP-2. It cross reacts with recombinant human MMP-2. Recombinant mouse MMP-3 protein, mouse MMP­-12 protein and rat MMP-­9 protein was also tested and no significant cross reactivity was observed.

Boster Scientific Support

Answered: 2016-09-12

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2015-09-05

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2015-09-05

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