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Product Info Summary
| SKU: | PROTQ29055 |
|---|---|
| Size: | 5ug,20ug |
| Origin Species: | Swine |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Swine recombinant IL-10 (Interleukin-10) protein, AF
View all IL10 recombinant proteins
SKU/Catalog Number
PROTQ29055
Size
5ug,20ug
Tag
His Tag (C-term)
Description
Interleukin 10 (IL-10) is a 17.54 kDa cytokine with 158 amino acid residues and is also known as human cytokine synthesis inhibitory factor (CSIF). IL-10 is an anti-inflammatory cytokine that is mainly secreted from monocytes, lymphocytes, and mast cells. IL-10 participates in the survival, proliferation, and antibody production of B cell via the JAK-STAT signaling pathway and suppress pro-inflammatory cytokine secretion by blocking the NF-κB signaling pathway.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Swine recombinant IL-10 (Interleukin-10) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ29055)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Molecular weight
The protein has a calculated MW of 19.05 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce proliferation in MC/9‑2 cells. The ED₅₀ for this effect is <5 ng/mL.
Endotoxin
<0.01 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MSIKSENSCIHFPTSLPHMLRELRAAFGPVKSFFQTKDQMGDLLLTGSLLEDFKGYLGCQALSEMIQFYLEDVMPKAESDGEDIKEHVNSLGEKLKTLRLRLRRCHQFLPCENKSKAVEEVKSAFSKLQERGVYKAMGEFDIFINYIEAYMTMKMRKN with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant swine IL-10
Specific Publications For Swine recombinant IL-10 (Interleukin-10) protein, AF (PROTQ29055)
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