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Product Info Summary
| SKU: | M10533 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122
SKU/Catalog Number
M10533
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122 (Catalog # M10533). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10533)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM122
Isotype
Rabbit IgG
Immunogen
Human IgE
Cross-reactivity
This antibody reacts to human IgE. No cross-reactivity with human IgG, IgM, IgD, or IgA.
Reactive Species
M10533 is reactive to IGHE in Human
Calculated molecular weight
47.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10533 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 10ng/well – 100ng/well (for Capture)
0.01ug/mL – 0.1ug/mL (for Detection)
Validation Images & Assay Conditions
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ELISA result showing specificity
Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM122 reacted to the IgE from human myeloma plasma and the IgE from human plasma. No cross reactivity with human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM122. The plate was coated with different amounts of human IgE (from plasma). A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122 (M10533)
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Customer Q&As
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4 Customer Q&As for Anti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122
Question
Do you have a BSA free version of anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 M10533 available?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 M10533 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-25
Question
I see that the anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 M10533 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-06
Question
We are currently using anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 M10533 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
D. Zhang
Verified customer
Asked: 2018-01-26
Answer
The anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 (M10533) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-26
Question
Is this M10533 anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 reactive to the isotypes of IGHE?
M. Mitchell
Verified customer
Asked: 2015-09-18
Answer
The immunogen of M10533 anti-Human IgE Rabbit Monoclonal antibody, Clone#RM122 is Human IgE. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-18
