Goat Anti-Mouse IgM Secondary Antibody, Biotin Conjugated

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgM. Cited in 6 publication(s).

Product Info Summary

SKU: BA1004
Size: 0.25ml
Reactive Species: Mouse
Host: Goat
Application: ELISA, IHC

Product Overview

Product Name Goat Anti-Mouse IgM Secondary Antibody, Biotin Conjugate
Synonyms Biotin-conjugated Goat Anti-Mouse IgM; Goat Anti-Mouse IgM Biotinylated Antibody; Biotinylated Goat Anti-Mouse IgM Secondary Antibody; Goat Anti-Mouse IgM Secondary Antibody, Biotin-labeled
Description Goat Anti-Mouse IgM Secondary Antibody, Biotin Conjugate, for indirect sensitive immunodetection and/or quantification of low-abundance target proteins through ELISA, IHC by using reporter-labeled biotin-binding signal amplification systems.
Reagent Type Biotin conjugated secondary antibody
Conjugate Biotin
Host Goat
Target Species Mouse
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule mouse IgM
Purification Immunoaffinity chromatography
Specificity Mouse IgM specific; no cross-reactivity with mouse IgG
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.25 mg biotin-conjugated secondary antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA, IHC
Storage At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type Human primary-IgM-probed: SDS-PAGE separated-, membrane-immobilized-proteins from cell/tissue lysates, formalin-fixed paraffin-embedded (FFPE) tissue sections on slides
Assay Type Immunoanalytical
Technique Indirect immunodetection of target protein via reporter-labeled biotin-binding detection systems
Assay Purpose Protein detection/quantification
Equipment Needed WB/Dot blot/ELISA/IHC instrumentation; Reporter signal detectors: X-ray film cassette; a charge-coupled device (CCD) imager; Spectrophotometer; fluorescent or electron microscope

Main Advantages

Specific High signal-to-noise ratio
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody; Multiple reporter molecules localize to a single biotin via avidin/streptavidin bridges
Fast Fewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible Biotin- (Strept)Avidin system can be coupled with various types of reporters (enzymes, fluorochromes, fluorophores, chromophores, etc.); One type of labeled secondary antibody can be used to recognize different types of primary antibodies of the target organism specific to a particular antigen
Compatible Biotin does not interfere with catalysis or antibody binding

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Biotinylated antibodies are widely used in systems where signal amplification is desired. Often 15-20 biotin moieties are coupled to a single IgG secondary antibody. Biotin binds avidin, streptavidin, or neutravidin with a high degree of affinity and specificity. In immunoassays avidin/streptavidin-biotin binding is used as a bridge between antibodies and reporters like enzymes (HRP, AP), fluorophores, chromophores, etc. Both avidin and streptavidin are tetrameric proteins capable of binding 4 biotin groups to each molecule of avidin or streptavidin, thus amplifying the signal intensity and detection sensitivity by increasing the concentration of reporters at the antigenic site. Two main biotin-binding detection systems have been widely utilized: Avidin-Biotin Complex (ABC) and Labeled Streptavidin Biotin (LSAB) methods. In the ABC method free avidin (or streptavidin) is used as a bridge/link between the biotinylated antibody and а biotinylated reporter molecule, resulting in three reporter molecules coupled to the biotinylated antibody. The LSAB method employs a reporter-labeled streptavidin (avidin or neutravidin can alternatively be used) to detect the bound biotinylated-secondary antibody on the tissue section, blotting membrane or ELISA plate, improving the sensitivity of detection by 8-fold. The LSAB method is used when the avidin-biotin-enzyme complex in the ABC method becomes too large to penetrate the tissue.

Validation Images & Assay Conditions

Hello CJ!

BA1004 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Exosomes derived from endoplasmic reticulum‑stressed liver cancer cells enhance the expression of cytokines in macrophages via the STAT3 signaling pathway

Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver

Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-κB in Acute Transection Spinal Cord Injury in Rats

Systematic evaluation of a tissue-engineered bone for maxillary sinus augmentation in large animal canine model

Zeng Y,Hua YQ,Wang W,Zhang H,Xu XL.Modulation of SIRT1-mediated signaling cascades in the liver contributes to the amelioration of nonalcoholic steatohepatitis in high fat fed middle-aged LDL receptor knockout mice by dihydromyricetin.Biochem Pharmacol.20
Species: Mouse
BA1004 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA

Li M, Liu Wj, Lu B, Wang Yh, Liu Jg. Acta Pharmacol Sin. 2013 Aug;34(8):1013-24. Doi: 10.1038/Aps.2013.28. Epub 2013 May 27. Differential Expression Of Arc In The Mesocorticolimbic System Is Involved In Drug And Natural Rewarding Behavior In Rats.

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SKU:BA1004

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