Biotin Conjugated Protein A

Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.
Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including immunohistochemistry and ELISA applications. Cited in 4 publication(s).

Product Info Summary

SKU: BA1025
Size: 0.5ml
Application: ELISA, IHC

Product NameBiotin Conjugated Protein A
SKU/Catalog NumberBA1025
Pack Size0.5ml
FormConcentrated, Liquid
Concentration1 mg/mL
DescriptionProtein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.
Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including immunohistochemistry and ELISA applications.
Contents0.5 mg of biotin conjugated protein A, Biotin: Ab=10:1, 0.01 M PBS, 50% glycerol.
Labeling MethodProtein A is conjugated to biotin by means of a method described by Bayer.
(Reference: Bayer,E.A. et al.,Meth.Enzymol.,62,308 (1979))
Storage & ExpirationUpon receipt store Biotin Conjugated Protein A at -20°C. Biotin Conjugated Protein A is stable for one year. Product is shipped on ice.
ApplicationsELISA, IHC
Cite This ProductBiotin Conjugated Protein A (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1025)

Validation Images & Assay Conditions

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BA1025 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sulforaphane and myricetin act synergistically to induce apoptosis in 3T3‑L1 adipocytes

Elevated expression of yes‑associated protein is associated with the malignant status and prognosis of laryngeal squamous cell carcinoma

Sulforaphane and myricetin act synergistically to induce apoptosis in 3T3%u2011L1 adipocytes

Li Ql, Huang N, Chen C, Chen Jl, Xiong Kq, Chen Jy, You Tx, Jin J, Liang X. J Biomed Mater Res A. 2010 Sep 15;94(4):1283-93. Doi: 10.1002/Jbm.A.32812. Oriented Immobilization Of Anti-Cd34 Antibody On Titanium Surface For Self-Endothelialization In...

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