This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about ATP-binding cassette sub-family B member 10, mitochondrial.
.
Human | |
---|---|
Gene Name: | ABCB10 |
Uniprot: | Q9NRK6 |
Entrez: | 23456 |
Belongs to: |
---|
ABC transporter superfamily |
ABC transporter 10 protein; ATP-binding cassette, sub-family B (MDR/TAP), member 10; EC 3.6.3; EC 3.6.3.44; EST20237; M-ABC2ATP-binding cassette transporter 10; Mitochondrial ATP-binding cassette 2; MTABC2ATP-binding cassette sub-family B member 10, mitochondrial
Mass (kDA):
79.148 kDA
Human | |
---|---|
Location: | 1q42.13 |
Sequence: | 1; NC_000001.11 (229516582..229558988, complement) |
Ubiquitous. Highly expressed in bone marrow, expressed at intermediate to high levels in skeletal muscle, small intestine, thyroid, heart, brain, placenta, liver, pancreas, prostate, testis, ovary, leukocyte, stomach, spinal cord, lymph node, trachea and adrenal gland, and low levels are found in lung, kidney, spleen, thymus and colon.
Mitochondrion inner membrane; Multi-pass membrane protein.
The ABCB10 gene is a common candidate for protein labeling in many studies. Its widespread application in many industries and research fields has made this a key tool in many areas. It has many uses, including gene mapping, autoradiography and special sample preparations. This article will help you to use this marker. Continue reading to find out more.
Boster bio's ABCB10 marker provides a target for the ABCB10 protein within biological samples. The Bosterbio RIPA Lysis buffer reagent can be used to detect the protein. It is a highly compatible buffer for the culture and lysis of mammalian tissues. Boster bio RIPA buffer has been validated in Western Blotting and Immunohistochemistry. It can be reused.
Western blot can be used to detect and analyze protein samples. It uses a polyacrylamide gel for the identification of proteins. The gel is then reformulated with secondary antibodies. Secondary antibodies are labeled using a radioisotope or fluorescent dye and used to identify the protein positions. Western blotting can also be used to confirm positive ELISA tests.
Abcb10, a ATPase, is also available. A deletion mutant in this gene lacks mTP, which is critical for proper importation of the molecule into the mitochondria. ABCB10 is less effective if the hydrophobic property is disrupted. This mutation could be saved by downstream mitochondrial targeting signal. Also, mass-spectrometry analysis of ABCB10 chemically crossed-linked immunoprecipitated ABCB10 proved that it homodimerizes.
The ABCB10 proteins is involved in the synthesis erythroid haglobin and the process of heme formation. Boster bio assays have shown that the enzyme, abcb10, binds to the heme containing cytoplasm of erythroid cells. The enzyme is necessary for the synthesis and use of erythroid-heme.
An ELISA test for the ABCB10 marker can be used to measure the human protein ABCB10. The ELISA kit can be used to measure the levels of human ABCB10 within biological fluids. The assay uses high-affinity antibody that can wash away non-specific binding materials. This method is a powerful tool to determine the ABCB10 mark's concentration in biological samples.
ELISAs use sandwich technology. Antibodies are pre-coated onto the plates. The wells are then filled with a biotin reagent, which is followed by a HRP reagent. Unbound conjugates can be removed using wash buffer at each stage. The TMB substrate quantifies HRP. After adding the substrate, a blue colour will appear. The sample is then diluted with an acidic stop solution and the colour changes into a yellow color. The amount ABCB10 attached to the plate is proportional to the yellow colour.
ELISAs for ABCB10 markers are available in sandwich and direct-capture formats. The target antigen is captured differently in both sandwich and direct capture formats. Direct capture ELISASs immobilize the target protein directly onto the assay plates. Sandwich ELISAs use a second antibody to immobilize the target antigen indirectly. Sandwich ELISAs are very common because they use two separate antigen layers to detect the antigen.
CusAb established a transmembrane proteins expression system in WB, IP ELISA and for the ABCB10 marker. This system can generate up to 35000 transmembrane products. CusAb has demonstrated the antibody's ability to react against rabbit, mouse and rat blood samples. It is unlikely that the antibody will detect biliverdin within human samples.
Boster Bio: ABCB10 refers to a gene whose expression is closely related to certain enzyme activity. It can be used in detecting a wide range if biomarkers. Boster's gene-based ELISA kit detects human and mouse ABCB10. The antibodies of the company have been tested for picogram-level sensitivities.
A variety of methods are available to detect the ABCB10 signal. Whole cell lysates are prepared from whole cells of rat cardiac muscles, including COLO320 and 22RV1. Below is how to detect the ABCB10 marker using monoclonal antibody. Both of these methods are useful for detecting the ABCB10 protein in various cell types.
Multiple studies have shown that c-AbcB10 levels in cells were increased by overexpression of the ABCB10 genes. Overexpression of the ABCB10 gene increased b-catenin gene expression, resulting in enhanced proliferation of tumor cells and activation of matrix metalloproteinase-2. These results support the notion that ABCB10 is essential for the malignant progression EOC.
Other studies have suggested that miR-1271 reversed the proproliferative effect of circ-ABCB10 in NSCLC. These results are consistent with previous studies, which showed a significant association between miR-128-3p and circ-ABCB10 expression. However, miR-128-3p expression appears to be significantly lower in cervical epithelial cancer cells than in CC tumour tissues.
The expression of CircaABCB10 is negatively correlated with the miR-584-4p gene in NSCLC tissues. This suggests that circABCB10 may be an endogenous miRNA sponge, which plays a significant role in tumor progression. In this way, detecting tumor cells using this marker can help physicians better understand the process of cancer and other diseases. However, this marker can only detect tumor cells. It requires more information and complex analyses.
Table 2 describes the detection techniques for the ABCB-10 mark.
PMID: 10922475 by Zhang F., et al. M-ABC2, a new human mitochondrial ATP-binding cassette membrane protein.
PMID: 7766993 by Allikmets R., et al. Characterization and mapping of three new mammalian ATP-binding transporter genes from an EST database.