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- Table of Contents
Facts about Arf-GAP domain and FG repeat-containing protein 1.
In the event of disease by HIV-1, functions as a cofactor for viral Rev and promotes motion of Rev-responsive element- containing RNAs in the nuclear periphery to the cytoplasm. This step is vital for HIV-1 replication.
Human | |
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Gene Name: | AGFG1 |
Uniprot: | P52594 |
Entrez: | 3267 |
Belongs to: |
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No superfamily |
ArfGAP with FG repeats 1; DKFZp686I15205; HIV-1 Rev-binding protein; HRBRev interacting protein; Rab, Rev/Rex activation domain-binding protein; RABMGC116938; RIPMGC116940
Mass (kDA):
58.26 kDA
Human | |
---|---|
Location: | 2q36.3 |
Sequence: | 2; NC_000002.12 (227472169..227561222) |
Ubiquitously expressed.
Nucleus. Cytoplasmic vesicle.
This article will cover the best uses of Anti-AGFG1 Marker. You can read more about the BAx(S4) Marker or Picokine ELISA to find other biomarkers. All of these can be used by any scientist, regardless of their background. Continue reading to find out more. Boster Bio: The Best Uses Of The AGFG1 Marker
Boster Bio Anti AGFG1 Marker Rat is a high affinity antibody reagent, which reacts with human AGFG1 gene. The antibodies have been validated on ELISA, Western Blotting and Immunohistochemistry. These results are consistent in the Boster-antibody used in this research. The antibody can be stored at -20°C for one year.
The circAGFG1 genome functions as a CCNE1-mediated ceRNA that promotes metastasis and proliferation. The gene acts as a decoy miR-195-5p. This study shows that circAGFG1 has been shown to be a prognosticator in TNBC. This gene is a potential biomarker that can be used in clinical trials to monitor the progression of this disease.
Geneseed Biotech fabricated an anti AGFG1 probe and a controlling probe. They incubated MDA-MB-231 cells with the specific probes and miR-195-5p mimics. The RNA complex of the probes and mimics was incubated with MDA-MB-231 cells. Next, the TRIzol method was used to purify it. This allowed them to measure its relative luciferase enzyme activity. Once the circAGFG1 marker was confirmed, it was subsequently used to analyze miR-195-5p expression.
In situ hybridization was used to determine the relative expression levels of circAGFG1 and TNBC tissues in a recent study. The Digoxin probe from Geneseed, Guangzhou, China was applied to TMAs. The tissues were then soaked in xylene and rehydrated with gradient alcohol. Finally, they were incubated overnight in anti-Digoxin AP.
A study was performed using mouse models to validate the monoclonal efficacy of circulating AGFG1 for cancer treatment. The results showed increased metastasis in mice by overexpression of circAGFG1 in mice. The knockdown of the gene also resulted in tumor growth being inhibited and decreased microvessel count. It is possible that circAGFG1 might play a role as regulating the cell cycle pathway.
Transwell assays were also used in the study to assess the effects of circAGFG1 upon TNBC cells. Knockdown or deletion of circAGFG1 resulted in a significant decrease in the expression of the proapoptotic protein Bax and Bcl-2. In vitro, however, knockup of circAGFG1 promoted TNBC cell growth. These studies show that circAGFG1 plays an essential role in tumor growth.
Picokine(tm), a Picokine(tm), ELISA-kit line, is a result of 20 year of research. Picokine technology improves the binding efficiency and decreases non-specific binding. This combination results in ELISA kits that have high specificity and picogram-level sensitivity. Picokine(tm), an ELISA platform, detects a variety of proteins and antigens. Each kit is rigorously inspected to ensure high performance.
The kit was verified against the Boster human IL-1 PikokineTM ELISA. The kit includes a 100 uL volume, controls, and standard solutions. The O.D. The O.D. is then performed using a microplate scanner. This kit was successfully validated in 18 of the 62 patients. The results were consistent across patient groups. Picokine ELISA is a convenient way to determine the IL-1 level of blood.
Picokine ELISA platforms were developed using insights in immunogen design and high affinity antibodies that can efficiently measure the protein even in crude preparations. This sensitive platform can detect the target protein even when it is not specific. Researchers can now combine multiple ELISA units to conduct experiments for the very first time. They can now determine the presence or absence of a protein, such as apoptosis-associated antigen.
This method uses one antibody rather than two, which reduces steps. Cross-reactivity can be eliminated by not labeling the primary antibody. Labeling primary antibodies is costly and time-consuming. This method can be costly and time-consuming. However, it offers the advantage of a limited supply of commercially available conjugated antibodies and a large number of labeled secondary antibody. The primary antibody retains the maximum immunoreactivity.
R&D Systems is a world leader in immunoassays, producing high-quality antibodies and antibody arrays. The company also produces cell-based assays, and serves the life science industry. This unique platform allows scientists to analyze a wide range of proteins and antigens, including those affecting the immune system. Picokine ELISA allows researchers to quickly identify the presence and absence of a particular protein.
The platform uses chemiluminescent and fluorescent substrates. These substrates must have a minimum of 400 ng/cm2 protein binding capacity and are HRP-dependent. This ensures a wide range of detection. Typically, substrates are coated at a lower level than the enzyme's maximum binding ability. This prevents nonspecific binding and hooking, which is a phenomenon caused by protein trapped between the coating proteins and inhibiting effective washing.
The AGFG1 genes codes for a protein with FG repeats. This protein is closely related the nucleoporins class of proteins that mediate nuclear RNA export. The protein binds with the activation domain (Rev protein), allowing it transport RNA from nucleus to cytoplasm. The full length of the AGFG1 gene is unknown. However, other transcript variants have also been identified in genome. To ensure its high specificity as well as affinity, the Boster antibody has been validated on samples with or without known positive or adverse reactions.
PMID: 7634337 by Bogerd H.P., et al. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins.
PMID: 7637788 by Fritz C.C., et al. A human nucleoporin-like protein that specifically interacts with HIV Rev.