APPL1 Antibodies

DCC-interacting protein 13-alpha (APPL1)

Adapter protein that interacts with proteins involved in various cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment.

Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway.

Gene Information

Human
Gene Name:	APPL1
Uniprot:	Q9UKG1
Entrez:	26060

Family

Belongs to:
No superfamily

Alternative Names

Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1; AKT2 interactor; APPL; APPL1; APPLdip13-alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13A; DIP13alpha; Dip13-alpha; KIAA1428; signaling adaptor protein DIP13alpha

Molecular Weight

Mass (kDA):
79.663 kDA

Genomic Context

Human
Location:	3p14.3
Sequence:	3; NC_000003.12 (57227729..57273471)

Tissue Specificity

High levels in heart, ovary, pancreas and skeletal muscle.

Subcellular Localizations

Early endosome membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endosome. Cell projection, ruffle. Cytoplasmic vesicle, phagosome. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.

Diseases

• Insulin Resistance
• Diabetes Mellitus
• Obesity
• Diabetes Mellitus, Non-insulin-dependent
• Insulin Sensitivity
• Oculocerebrorenal Syndrome
• Inflammation
• Nervousness
• Dent's Disease
• Malignant Neoplasms
• Hyperglycemia

• Carcinoma
• Cardiovascular Diseases
• Fanconi Syndrome
• Tissue Adhesions
• Atherosclerosis
• Cholangiocarcinoma

Interacting Proteins

• ACTN2
• ADIPOR1
• Adipor1
• ADIPOR2
• AKT1

• APPL2
• DST
• DYSF
• EGFR
• FARS2

• HDAC1
• HDAC3
• MAGEA9
• MEOX1
• RAB21

• RAB5A
• RHEBL1
• RTCB
• SYNE2
• TRAF2

• UBE2O

Pathways

• Endocytosis
• Localization
• Transport
• Secretion
• Cell Proliferation
• Glucose Transport
• Vasodilation
• Insulin Secretion
• Energy Homeostasis
• Rna Interference
• Clathrin-mediated Endocytosis
• Gluconeogenesis
• Sensitization

• Cell Cycle
• Intracellular Signal Transduction
• Cell Growth
• Fatty Acid Oxidation
• Cell Adhesion
• Osteoclast Differentiation
• Cell Migration

PTMs

• Phosphorylation
• Oxidation
• Nitration

• Deacetylation
• Cleavage
• Dephosphorylation

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/APPL1

Best Uses Of The APPL1 Marker From Boster Bio

When performing research on one particular species or gene for example, a specialized antibody such as the APPL1 marker made by Boster Bio is highly beneficial. Scientists are encouraged submit their research results for product credits. Additionally, Boster scientists can submit their results for specific species-specific samples and applications as well. This product is accessible to every scientist in the world. It has many benefits and can be utilized to different types of gene-related research and species.

APPL1

The protein APPL1 is encoded by the DCC interfering protein 13 alpha gene. It's it is mapped to 3q21.1-p13.3. The protein contains seven hundred nine amino acids and shares 54 percent amino acid identity with APPL2. It is abundantly expressed in tissues that have abundant AKT2 mRNA. APPL is regarded as an adaptor. It may act as a tether to bind active PI3K and PI2K in the cytoplasm. The Boster antibody has been tested for use in flow-cytometry, IHC-P, and Immunofluorescence.

APPL1 gene

The APPL1 gene encodes the protein DCC-interacting protein 13 alpha (APPL1). The APPL1 gene resides at 3q21.1-p13.3 and has 709 amino acids. It shares 54% amino acids with APPL2. It is extensively expressed in tissues with the highest levels of AKT2 mRNA. It is regarded as an adaptor protein, and is able to bind inactive PI3K and AKT2 within the cytoplasm. Boster Bio's APPL1 marker is confirmed in IHC as well as WB and Immunofluorescence.

APPL1 antibody

APPL1 antibodyfrom Boster Bio product, is a reliable way to target this protein. This antibody has been demonstrated to reduce a variety of behavioral tests in mice such as mechanical hyperalgesia. APPL1 is also involved in the regulation of mTOR. Numerous studies have proven that the protein affects synaptic plasticity and pain responses in various models. This boster bio product is a great option for research in this area.

APPL1 is co-expressed with NF200 which is a marker for myelinated A-fibers. This protein was found to be less abundant in PDN rats. The Boster Bio APPL1 antibody can detect this protein in various cell types. The antigen comes from the brain tissue of a rat. It is then purified. This Boster Bio product can be used in various animal models to further study.

The expression of APPL1 is controlled by p-mTOR in the brain. Normal rats express APPL1 in the spinal cord, microglia, and brain. In PDN rats the APPL1 gene is less abundant and p-mTOR levels are higher. The protein is also deregulated in the lamina of the spinal dorsal horn. It is helpful in studies on the mTOR-mediated neuropathy.

In addition to ELISA kits, Boster Biologicals develops antibodies for research. Boster APPL1 antibody can detect biomarkers for cancer, developmental neurosciences, biology as well as inflammation and more. It is sensitive to picogram levels. It is compatible with multiple tests and is supported by technical assistance. These products can be found on Tebubio. They are affordable.

APPL1 vesicle pools

The APPL1 gene encodes the DCC-interacting protein 13 alpha, having 709 amino acids and 54% resemblance to APPL2. It is extremely abundant in tissues that contain high levels of AKT2 mRNA. APPL is thought to be an adaptor and may be a receptor for binding AKT2 to PI3K in the cytoplasm. Antibodies to APPL1 have been validated by flow cytometry and Immunofluorescence.

APPL1-positive cells are located close to the plasma membrane. Vesicles that are APPL1-negative reside in a subdomain of the EEA1+ endosomes. Both WDFY2 (and APPL1) were localized to distinct subdomains within VEsicles. Additionally, VEsicles enriched with WDFY2 are known to contain large amounts of this protein.

The WDFY2 gene is typically deep deleted in tumors, with frequencies grouped according to the kind of cancer. WDFY2 is required for VAMP3, an anchor matrix metalloprotease in the membrane which is recycled. When WDFY2 is eliminated VAMP3 moves to lysosomes to be degraded. The loss of WDFY2 permits enhanced recycling of VAMP3 and results in more vesicles showing a an identity of secretory. Loss of WDFY2 results in an increase in the secretion of MT1-MMP, which is vital for cell invasion and degradation.

APPL1 gene infographic

The gene APPL1 encodes a protein known as DCC-interacting protein 13 alpha. The gene is located at 3q21.1-p13.3 and contains 709 amino acids. The protein shares 54% amino acids identity with the APPL2 protein. The APPL1 protein can be located in tissues with AKT2 mRNA in high amounts. It is known to function as an adaptor protein and tethers inactive AKT2 to the PI3K in the cytoplasm. The Boster antibody has been validated using immunofluorescence, western blot as well as immunohistochemistry.