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- Table of Contents
CBX7 / Chromobox protein homolog 7 · Western blot design guide
Design a Western Blot for CBX7
Source-linked CBX7 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CBX7 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CBX7 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~28.3 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Colon (IHC candidate; verify WB) +4 more | |
| Negative control | Adrenal gland (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked CBX7 Western Blot Protocol Options
The M04742 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | HepG2 cell lysate (catalog M04742) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Short semi-dry transfer; verify retention (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M04742; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected CBX7 Western Blot Band Size?
CBX7 is predicted at 28.3 kDa; nuclear localization may affect recovery, but no empirical band size or migration difference is established.
| Single band near 28.3 kDa | Consistent with the predicted CBX7 mass; confirm its identity |
| Band near 28.3 kDa in nuclear extract | Consistent with CBX7 nuclear localization |
| Faint band near 28.3 kDa in whole-cell lysate | CBX7 detection may depend on nuclear protein recovery |
| Little or no band in cytoplasmic fraction | Consistent with CBX7 nuclear localization |
💡Expected CBX7 appearanceCBX7 has a predicted mass of 28.3 kDa, but no empirical band size is supplied; confirm any candidate band with antibody and sample controls.
| UniProt predicted mass | Places the candidate CBX7 band near 28.3 kDa |
| Predicted mass in daltons | 28,341 Da corresponds to approximately 28.3 kDa |
| Predicted full-length mass | Provides a 28.3 kDa reference for comparing candidate bands |
| Predicted monomer mass | Provides a 28.3 kDa reference; actual migration is unreported |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear CBX7 may be poorly recovered | Check nuclear protein recovery and a positive control |
| Band higher than expected | Identity of the larger band is unconfirmed | Compare with a second CBX7 antibody or CBX7 depletion |
| Band lower than expected | Identity of the smaller band is unconfirmed | Check sample integrity and confirm with CBX7 depletion |
| Multiple bands | The supplied features do not establish multiple CBX7 forms | Identify which band changes with CBX7 depletion |
| Weak or no signal | Low nuclear protein recovery or limited antibody detection | Check nuclear recovery and an antibody positive control |
Experiment evidence
Western blot images showing CBX7 band
M04742 · CBX7
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CBX7 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CBX7 in Western blot, you can use colon tissue, which HPA rates as highly positive.
Positive control: Colon (IHC candidate; verify WB)
Negative control: Adrenal gland (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: Because CBX7 is nuclear, nuclear-enriched lysate may improve detection.
HPA tissue expression evidence for CBX7
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | endothelial cells | High | Protein (IHC) | HPA → |
| Lung | alveolar cells type II | High | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Appendix | lymphoid tissue | Medium | Protein (IHC) | HPA → |
| Breast | myoepithelial cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Duodenum | glandular cells | Not detected | Protein (IHC) | HPA → |
| Esophagus | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CBX7 Western Blot Tips
Deeper troubleshooting and optimisation questions for CBX7, answered from its protein features.
Where should the main CBX7 band appear?
Band shift · The supplied sequence is 251 residues with a predicted mass of 28.3 kDa. Use 28.3 kDa as a reference when identifying a band; no observed band position is supplied.
Should multiple CBX7 isoform bands be expected?
Isoforms · The supplied record lists one isoform and no alternative sequence. It provides no basis for assigning multiple bands to annotated CBX7 isoforms.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of CBX7?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for CBX7 Western blot?
Transfer · Plan transfer around the predicted 28.3 kDa protein and check that it is retained on the membrane. The supplied features do not specify a membrane type or transfer settings.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M04742 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should CBX7 bands be quantified?
Quantitation · Because CBX7 is annotated as nuclear, compare like sample fractions across lanes. Identify the band being measured against the 28.3 kDa prediction, while recognizing that no empirical band position is supplied.
Does CBX7 have a documented cause of a band shift?
Interpretation · The supplied features list no modified residues, glycosylation sites, signal peptide, or propeptide. They do not establish a visible shift or explain any difference between apparent and predicted mass.
CBX7 is annotated as nuclear. A nuclear fraction is therefore a relevant sample to examine when checking for CBX7 expression.
CBX7 is a component of a PRC1-like complex and interacts with RING1, RNF2/RING1B, and several PCGF proteins. Those interactions alone do not identify an unexpected Western-blot band as CBX7.
CBX7 is annotated as nuclear. A nuclear fraction is therefore a relevant sample to examine when checking for CBX7 expression.
CBX7 is a component of a PRC1-like complex and interacts with RING1, RNF2/RING1B, and several PCGF proteins. Those interactions alone do not identify an unexpected Western-blot band as CBX7.
Boster reagents
CBX7 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-cbx7 Monoclonal Antibody
Cat # M04742
The catalog reports one anti-CBX7 antibody, M04742, with reported human reactivity and a Western blot image showing CBX7 expression in HepG2 cell lysate. The supplied evidence describes this tested context only; broader sample validation is not provided.
Which to pick: M04742 is the only listed option. Its Western blot image uses HepG2 cell lysate, making it the documented choice for that context. Check suitability for other samples separately.
Source: BosterBio CBX7 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O95931.
- Human Protein Atlas. CBX7 tissue expression.
- PMC5503594 — target-verified WB comparison
- PMC11094083 — target-verified WB comparison
- PMC5432285 — target-verified WB comparison