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- Table of Contents
CD37 / Leukocyte antigen CD37 · Western blot design guide
Design a Western Blot for CD37
Source-linked CD37 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-CD37 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
CD37 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~31.7 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked CD37 Western Blot Protocol Options
The A05276 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | HeLa cells (catalog A05276) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A05276; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | 1:20000 (catalog A05276) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected CD37 Western Blot Band Size?
CD37 has a predicted mass of 31.7 kDa; glycosylation and isoforms may affect migration, but no empirical band size or distinct pattern is established.
| Band near 31.7 kDa | consistent with the predicted CD37 sequence mass; migration is unverified |
| Band above 31.7 kDa | could reflect N-linked glycosylation at Asn170, Asn183, or Asn188 |
| Several bands at different positions | could reflect isoforms 1, 2, and 3, if their sizes differ enough to resolve |
| Broad band or smear | could reflect heterogeneous glycosylation at the annotated N-linked sites |
💡Expected CD37 appearanceCD37 has a predicted sequence mass of 31.7 kDa, but no empirical band size is supplied; confirm any candidate band with appropriate positive and negative controls.
| Predicted CD37 mass | 31.7 kDa is the sequence-based reference, not a measured band |
| N-linked glycosylation at Asn170 | may increase apparent size if occupied |
| N-linked glycosylation at Asn183 | may increase apparent size if occupied |
| N-linked glycosylation at Asn188 | may increase apparent size if occupied |
| Isoforms 1, 2, and 3 | may differ in size, but their masses and migration order are unknown |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane-associated CD37 may be poorly recovered or solubilized | Check membrane extraction and run a CD37-positive control |
| Band higher than expected | N-linked glycosylation at the annotated asparagines is possible | Compare treated and untreated samples after N-glycan removal |
| Band lower than expected | An alternative isoform or an unrelated band is possible | Check band identity with positive and negative controls |
| Broad smear instead of sharp band | Variable N-linked glycosylation is possible | Compare samples before and after N-glycan removal |
| Multiple bands | Isoforms or differing glycosylation are possible | Compare N-glycan removal results and verify bands with controls |
Experiment evidence
Western blot images showing CD37 band
A05276 · CD37
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CD37 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for CD37 in Western blot, you can use appendix tissue, which HPA scores High.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside CD37.
⚠️Feasibility: As a multi-pass membrane protein, CD37 may give a clearer signal in membrane-enriched lysate.
HPA tissue expression evidence for CD37
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | germinal center cells | High | Protein (IHC) | HPA → |
| Kidney | proximal tubules (cell body) | High | Protein (IHC) | HPA → |
| Tonsil | non-germinal center cells | High | Protein (IHC) | HPA → |
| Colon | mucosal lymphoid cells | Medium | Protein (IHC) | HPA → |
| Lymph node | germinal center cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Not detected | Protein (IHC) | HPA → |
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced CD37 Western Blot Tips
Deeper troubleshooting and optimisation questions for CD37, answered from its protein features.
How should CD37 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
How could CD37 isoforms affect Western blot bands?
Isoforms · UniProt lists isoforms 1, 2, and 3. Isoforms 2 and 3 lack canonical residues 1–68. In isoform 3, canonical residues 257–281 are also replaced: LGFMTLSIFLCRNLDHVYNRLARYR becomes VIWPRPHPRSALNP. Compare expected isoform sequences before assigning bands.
Choose an epitope outside canonical residues 1–68 and 257–281 if the goal is to detect all three listed isoforms. Confirm the antibody’s stated epitope against each isoform sequence; the supplied UniProt features do not establish antibody performance.
Choose an epitope outside canonical residues 1–68 and 257–281 if the goal is to detect all three listed isoforms. Confirm the antibody’s stated epitope against each isoform sequence; the supplied UniProt features do not establish antibody performance.
Which CD37 glycosylation sites matter when interpreting bands?
PTM · UniProt annotates N-linked glycosylation at Asn170, Asn183, and Asn188, using canonical sequence coordinates. Consider these sites when evaluating band patterns, but their annotation alone does not prove that glycosylation causes a visible shift.
Does this guide establish induction of CD37?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for CD37 Western blot?
Transfer · CD37 is a 281-residue, multi-pass cell-membrane protein with a predicted canonical mass of 31.7 kDa. Optimize transfer by checking CD37 recovery on the membrane and retention in the gel. The supplied features do not identify one preferred transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A05276 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
What should be controlled when quantifying CD37 bands?
Quantitation · Quantify comparable bands across samples using the same isoform assignment and band-integration approach. CD37 has three listed isoforms and three annotated N-linked glycosylation sites, so document which bands are included when comparing signal.
Should CD37 run at its predicted 31.7 kDa mass?
Interpretation · 31.7 kDa is the predicted mass of canonical CD37. Its three annotated N-linked glycosylation sites and alternative isoforms may affect migration, but these features do not establish an apparent band size or explain a particular difference from 31.7 kDa.
First compare them with the annotated isoform changes and N-linked sites at canonical Asn170, Asn183, and Asn188. The listed interactions with SCIMP, SOCS3, and DECTIN1/CLEC7A do not by themselves identify an unexpected Western blot band. The supplied features provide no observed band size.
First compare them with the annotated isoform changes and N-linked sites at canonical Asn170, Asn183, and Asn188. The listed interactions with SCIMP, SOCS3, and DECTIN1/CLEC7A do not by themselves identify an unexpected Western blot band. The supplied features provide no observed band size.
Boster reagents
CD37 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Leukocyte antigen CD37 CD37 Antibody
Cat # A05276
A05276 is an anti-CD37 antibody listed for human, mouse, and rat. Its Western blot image shows HeLa cells, with secondary antibody diluted 1:20000. No publication evidence or mouse or rat blot is supplied.
Which to pick: A05276 is the only listed option and has a HeLa Western blot image. For mouse or rat samples, check suitability for your experiment; the supplied image does not show those samples.
Source: BosterBio CD37 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P11049.
- Human Protein Atlas. CD37 tissue expression.
- PMC12271527 — target-verified WB comparison
- PMC11331048 — target-verified WB comparison