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- Table of Contents
DLX3 / Homeobox protein DLX-3 · Western blot design guide
Design a Western Blot for DLX3
Source-linked DLX3 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-DLX3 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
DLX3 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~31.7 kDa | |
| Gel | 12–15% (standard starting point) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked DLX3 Western Blot Protocol Options
The M05844 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | JAR cell lysate (catalog M05844) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | M05844; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected DLX3 Western Blot Band Size?
DLX3 is predicted at 31.7 kDa; the supplied features establish no shifted migration or empirical band size, so confirm candidate bands with controls.
| Single band near 31.7 kDa | Consistent with the predicted DLX3 size; confirm identity with controls |
| No band in whole-cell lysate | DLX3 may be missed if nuclear protein recovery is poor |
| Band above 31.7 kDa | Its identity needs confirmation; the listed features do not explain the shift |
| Band below 31.7 kDa | Its identity needs confirmation; no cleavage feature is listed |
💡Expected DLX3 appearanceUniProt predicts a 31.7 kDa DLX3 band, but no empirical band size is supplied; confirm a candidate band with appropriate identity controls.
| Predicted molecular weight of 31.7 kDa | Sets the expected position for the full-length protein |
| Sequence mass of 31,738 Da | Corresponds to the same approximately 31.7 kDa expectation |
| 287-residue protein length | Describes the full-length chain; no shorter processed product is listed |
| MEIS1 heterodimer interaction | No effect on the SDS-PAGE band size is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | DLX3 is nuclear as well as cytoplasmic, and nuclear recovery may be poor | Check nuclear extraction and a positive-control lysate |
| Band higher than expected | The supplied features do not establish a size-increasing modification | Compare with a positive control and verify antibody specificity |
| Band lower than expected | The supplied features do not establish a cleaved product | Check sample integrity and verify the band with an independent antibody |
| Multiple bands | Only one isoform is listed, so additional bands lack a feature-based assignment | Compare positive and negative controls and validate candidate bands |
| Weak or no signal | DLX3 recovery or antibody detection may be insufficient | Check loading, nuclear recovery, and a positive control |
Experiment evidence
Western blot images showing DLX3 band
M05844 · DLX3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for DLX3 Western blot
🧪For positive controls for DLX3 in Western blot, you can use an independently validated DLX3-positive sample, because no HPA positive tissue or cell is supplied.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: HPA provides no tissue data, so tissue controls cannot be selected from the supplied evidence.
HPA tissue expression evidence for DLX3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced DLX3 Western Blot Tips
Deeper troubleshooting and optimisation questions for DLX3, answered from its protein features.
Where should the DLX3 band appear relative to predicted mass?
Band shift · DLX3 has a predicted mass of 31.7 kDa. No empirical band position is supplied, so use 31.7 kDa as a starting reference, not a confirmed apparent mass.
Could DLX3 isoforms explain multiple bands?
Isoforms · The supplied record lists one isoform and no alternative sequence. It provides no isoform-based explanation for multiple bands; verify any additional bands independently.
Do annotated modifications prove a band shift?
PTM · The linked UniProt record describes protein features. A modification annotation alone does not demonstrate a visible shift; retain any condition or experimental qualifier attached to it.
Does this guide establish induction of DLX3?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for DLX3 Western blot?
Transfer · DLX3 is predicted to be 31.7 kDa. Choose and verify transfer conditions suitable for a protein near 32 kDa; the supplied features do not specify a membrane or transfer settings.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M05844 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should DLX3 be quantified across samples?
Quantitation · Quantify the same validated DLX3 band across samples. Because DLX3 is annotated in both nucleus and cytoplasm, compare like fractions and account for fraction purity when measuring compartment-specific changes.
Which cell fractions should be checked for DLX3?
Interpretation · DLX3 is annotated in the nucleus and cytoplasm. Check both fractions if testing localization, and assess fraction purity before interpreting their relative signals.
DLX3 is reported to form a heterodimer with MEIS1 and to interact with IPO7. Those interactions alone do not identify an unexpected Western-blot band. Confirm its identity before assigning it to a DLX3 complex.
DLX3 is reported to form a heterodimer with MEIS1 and to interact with IPO7. Those interactions alone do not identify an unexpected Western-blot band. Confirm its identity before assigning it to a DLX3 complex.
Boster reagents
DLX3 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-DLX3 Rabbit Monoclonal Antibody
Cat # M05844
M05844 is a rabbit monoclonal anti-DLX3 antibody listed for human reactivity. Its Western blot image shows DLX3 expression in JAR cell lysate; the supplied evidence does not establish performance in other samples.
Which to pick: M05844 is the only listed DLX3 antibody. Choose it for a human Western blot when the reported JAR cell lysate result is relevant to your experiment.
Source: BosterBio DLX3 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O60479.
- Human Protein Atlas. DLX3 tissue expression.
- PMC4376716 — target-verified WB comparison
- PMC6256238 — target-verified WB comparison
- PMC2803028 — target-verified WB comparison