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- Table of Contents
DMP1 / Dentin matrix acidic phosphoprotein 1 · Western blot design guide
Design a Western Blot for DMP1
Real validated DMP1 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-DMP1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
DMP1 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~55.8 kDa | |
| Gel | 12–15% (standard starting point) | |
| Positive control | Adipose tissue (IHC candidate; verify WB) +4 more | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Phosphorylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated DMP1 Western Blot Protocols
The A01984 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | Target-positive lysate and matched negative control (standard starting point) |
| Gel % | 12–15% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Standard semi-dry transfer; verify efficiency (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A01984; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected DMP1 Western Blot Band Size?
DMP1 has a predicted 55.8 kDa precursor; signal-peptide removal, N-linked glycosylation, and isoforms could affect migration, but no empirical band size or effect is established.
| Band near 55.8 kDa | Consistent with the predicted DMP1 precursor mass; identity needs confirmation |
| Band slightly below 55.8 kDa | Could reflect removal of the 1–16 signal peptide |
| Band above 55.8 kDa | Could reflect N-linked glycosylation; its migration effect is unverified |
| More than one band | Isoforms 1 and 2 are possible contributors, but distinct migration is unverified |
| Little or no band in whole-cell lysate | DMP1 can localize to the extracellular matrix |
💡Expected DMP1 appearanceUniProt predicts a 55.8 kDa DMP1 precursor; no empirical band size is supplied, and signal-peptide removal, N-linked glycosylation, or isoforms could affect migration, so confirm band identity with appropriate controls.
| UniProt predicted mass | 55.8 kDa for the precursor; apparent migration is unverified |
| Signal peptide at residues 1–16 | Its removal could make mature DMP1 smaller than the precursor |
| N-linked glycosylation at Asn25, Asn285, Asn324, and Asn345 | Could increase apparent size or alter migration; a visible effect is unverified |
| N-linked glycosylation at Asn351, Asn413, Asn426, and Asn467 | Could increase apparent size or alter migration; a visible effect is unverified |
| Isoforms 1 and 2 | May differ in size, but their masses and migration difference are not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | DMP1 can be secreted into the extracellular matrix | Check conditioned medium or extracellular matrix alongside lysate |
| Band higher than expected | N-linked glycosylation is possible, but the cause of a shift is unverified | Compare with an appropriate glycosidase-treated sample and confirm band identity |
| Band lower than expected | Removal of the 1–16 signal peptide could lower precursor mass | Confirm band identity and check whether the antibody recognizes the detected form |
| Broad smear instead of sharp band | Heterogeneous N-linked glycosylation is possible but unverified | Compare glycosidase-treated and untreated samples |
| Multiple bands | Isoforms 1 and 2 could contribute, but distinct migration is unverified | Use an independent antibody or targeted knockdown to assess band identity |
| Weak or no signal | DMP1 location varies among nucleus, cytoplasm, and extracellular matrix | Compare cellular and extracellular fractions from the relevant cell state |
Experiment evidence
Western blot images showing DMP1 band
A01984 · DMP1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for DMP1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for DMP1 in Western blot, you can use adipose tissue, an HPA medium-expression tissue.
Positive control: Adipose tissue (IHC candidate; verify WB)
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: DMP1 is secreted, so whole-cell lysate may show little signal; consider conditioned medium.
HPA tissue expression evidence for DMP1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Medium | Protein (IHC) | HPA → |
| Breast | glandular cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | neuronal cells | Low | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Low | Protein (IHC) | HPA → |
| Lymph node | non-germinal center cells | Low | Protein (IHC) | HPA → |
| Ovary | ovarian stroma cells | Low | Protein (IHC) | HPA → |
| Salivary gland | glandular cells | Low | Protein (IHC) | HPA → |
Section 3
Advanced DMP1 Western Blot Tips
Deeper troubleshooting and optimisation questions for DMP1, answered from its protein features.
How should DMP1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could DMP1 isoforms produce different bands?
Isoforms · UniProt lists isoforms 1 and 2. Isoform 2 lacks residues 46–61 of the canonical sequence. Check whether the antibody’s epitope includes that region before interpreting a band as isoform 2; the sequence difference alone does not establish two visible bands.
Which DMP1 glycosylation sites matter when interpreting bands?
PTM · The supplied UniProt coordinates list N-linked glycosylation at Asn25, Asn285, Asn324, Asn345, Asn351, Asn413, Asn426, and Asn467. These are canonical-sequence coordinates; antibody or paper numbering may differ. Their annotation does not establish that glycosylation caused a particular band shift.
Does this guide establish induction of DMP1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for DMP1?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A01984 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should DMP1 be quantified across samples?
Quantitation · Keep cell stage and sample fraction consistent across comparisons. DMP1’s annotated location changes between proliferating preosteoblasts and early maturation, so a change in one fraction may reflect redistribution. Quantify the same defined band or bands across samples and report which fraction was measured.
Should DMP1 run at its predicted 55.8 kDa?
Interpretation · The 55.8 kDa prediction is for the 513-residue sequence, which includes a signal peptide at positions 1–16. DMP1 also has annotated glycosylation sites. These features alone cannot predict its apparent Western blot mass, and no observed band size was supplied.
DMP1 is annotated in the nucleus, cytoplasm, and extracellular matrix. The supplied location note places it in the nucleus of proliferating preosteoblasts and the cytoplasm during early maturation. Choose fractions to match the cell stage and location being studied, and compare like fractions.
Check whether the antibody recognizes the isoform 2 deletion at canonical residues 46–61, and consider the signal peptide at 1–16 and the annotated N-linked glycosylation sites. None of these features alone identifies an unexpected band. No empirical DMP1 band size was supplied for comparison.
DMP1 is annotated in the nucleus, cytoplasm, and extracellular matrix. The supplied location note places it in the nucleus of proliferating preosteoblasts and the cytoplasm during early maturation. Choose fractions to match the cell stage and location being studied, and compare like fractions.
Check whether the antibody recognizes the isoform 2 deletion at canonical residues 46–61, and consider the signal peptide at 1–16 and the annotated N-linked glycosylation sites. None of these features alone identifies an unexpected band. No empirical DMP1 band size was supplied for comparison.
Boster reagents
DMP1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-DMP1 Antibody
Cat # A01984
The catalog reports A01984, an anti-DMP1 polyclonal antibody with stated Human and Mouse reactivity and a Western blot image. The supplied record gives no sample details or validation conditions, so the image alone does not establish performance across both species.
Which to pick: A01984 is the only listed DMP1 antibody. It has a WB image and stated Human and Mouse reactivity; check the full image caption and conditions against your sample before choosing it.
Source: BosterBio DMP1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q13316.
- Human Protein Atlas. DMP1 tissue expression.
- PMC4361624 — target-verified WB comparison
- PMC3794354 — target-verified WB comparison
- PMC7093497 — target-verified WB comparison