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- Table of Contents
Facts about EH domain-containing protein 3.
Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport.
Human | |
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Gene Name: | EHD3 |
Uniprot: | Q9NZN3 |
Entrez: | 30845 |
Belongs to: |
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TRAFAC class dynamin-like GTPase superfamily |
EH domain containing 3; EH domain-containing protein 3; EHD2; EH-domain containing 3; PAST homolog 3; PAST3
Mass (kDA):
60.887 kDA
Human | |
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Location: | 2p23.1 |
Sequence: | 2; NC_000002.12 (31234152..31269451) |
Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.
Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, cilium membrane; Peripheral membrane protein; Cytoplasmic side. Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with RAB8A and MYO5B to a cytoplasmic tubular network devoid of RAB11A (By similarity). Colocalizes with ANK2 in myocyte perinuclear region (PubMed:20489164). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippoc
To learn more about Steven Boster, read his bio and explore his current research. Then, read on for some useful information about High-affinity primary antibodies and EHD3 tyrosine kinase inhibitors. These compounds are highly potent antibodies that target specific proteins. In addition, they are widely used for diagnostic applications and clinical trials. The EHD3 marker is one of Steven's top targets.
Steve Boster is a retired Emergency Medicine physician and painter from San Angelo, Texas. He has been painting full-time since 2015. His studio is located in the Chicken Farm Art Center in San Angelo. He enjoys painting animals in vibrant colors and works in a variety of styles, from realism to early impressionism. Boster has always been interested in the outdoors and hunting and he used to donate a portion of his sales to the Fisher House Foundation, which provides meals for wounded veterans.
The Boster bio was founded by Steven Boster. Boster earned the nickname "he who converts science in the lavatory" after his invention of an ELISA kit. The company was born in 1993, and by the late nineties, Boster had built the largest catalog antibody company in China. His company is now known for its proprietary PicoKine(tm) ELISA platform. The company uses proprietary trade secrets to create high-sensitivity ELISA kits.
In rice, the EHD3 gene encodes a nuclear protein with two PHD-finger motifs. It is a key promoter for a gene regulatory network that regulates photoperiodic flowering. In this study, we will identify the genes that affect photoperiodic flowering in rice. Among the other current projects using the EHD3 marker, some focus on the role of EHD3 in plant growth and development.
To understand the role of Ehd3 in flowering in plants, we have found that the gene functions as a floral inducer. Fifty days after germination, the shoot apices of wild-type plants differentiate into a young panicle. The mutant shoot apices, however, are still in the vegetative stage. Further mutagenesis will be required to detect suppressors of the EHD3 mutation.
High-affinity primary antibodies are produced using the EHD3 marker, a common DNA sequence. Antibodies are naturally occurring molecules that interact with specific epitopes on the target antigen. These antibodies have high affinity and may require more elution steps and harsher methods. Using these antibodies ensures more consistent results. In addition, they are less prone to contaminating other samples.
The binding affinity of EHD3 was measured at 17.8 +/ 3.7 mm, which is similar to that of Eh2 and Rabankyrin-5. Because affinity and KD are inversely related, the lower the KD value, the higher the affinity of the antibody. Data processing was performed at UC Davis and the OI-RD measurements were reviewed by Abcam scientists.
While there are many commercial sources for these antibodies, quality is a critical factor that may make or break a successful experiment. Purchasing direct from the manufacturer will ensure that you receive the highest-quality antibodies. Moreover, you will know exactly what you're getting. If an antibody doesn't work as expected, you can request additional tests. By using this method, you can identify specific proteins and molecules in a sample without the need for a second experiment.
TRE biogenesis is one of the main functions of EHDs. It is required for the formation of TRE vesicles and can promote membrane bending. In addition, it may also play a role in stabilizing the MICAL-L1*Syndapin2 complex. This means that high-affinity antibodies targeting EHD3 are useful in treating tremors and preventing bacterial infections.
Secondary antibodies are made by immunizing a host animal with a target antigen. In this case, goats immunized with purified mouse IgG will produce goat anti-mouse antibodies that bind to all of the mouse IgG fragments and classes. But if the goats are immunized with mouse IgG1 antibodies, they will only produce anti-mouse antibodies that are specific to this particular IgG class.
In order to produce high-affinity primary antibodies for EHD3-positive patients, researchers used 50 micrograms of total protein extracts of HIV-positive donors and healthy donors. These were transferred onto a PVDF membrane. Then, they were detected by enhanced chemiluminescence and X-rays. These antibodies were able to recognize EHD3-positive cells, as well as to detect apoptosis.
PMID: 10673336 by Pohl U., et al. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins.
PMID: 12121420 by Galperin E., et al. EHD3: a protein that resides in recycling tubular and vesicular membrane structures and interacts with EHD1.