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Facts about Free fatty acid receptor 2.
SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is likely coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, PubMed:12711604, PubMed:23589301).
Human | |
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Gene Name: | FFAR2 |
Uniprot: | O15552 |
Entrez: | 2867 |
Belongs to: |
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G-protein coupled receptor 1 family |
FFAR2; free fatty acid activated receptor 2; free fatty acid receptor 2; G protein-coupled receptor 43; GPR43; GPR43FFA2R; G-protein coupled receptor 43
Mass (kDA):
37.144 kDA
Human | |
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Location: | 19q13.12 |
Sequence: | 19; NC_000019.10 (35448257..35451767) |
Expressed at relatively high levels in peripheral blood leukocytes and, to lesser extent, in spleen.
Cell membrane; Multi-pass membrane protein.
If you're a beginner in biotechnology, you may wonder what the best methods are to optimize experiments. This article will provide an overview of the best ways to optimize experiments, including high-affinity primary antibodies as well as optimization techniques. Additionally, you will learn more about Boster Bio as well as the FFAR2 mark. For additional information about Boster Bio, read the following articles:
The FFAR2 transcription factor regulates many functions. SCFA, the major fiber digesting product activates it. FFAR2 plays a role in intestinal immunity and whole-body energy homeostasis. This gene is essential to many bodily processes and its important role in the immune function is of particular importance. Multiple studies have shown that FFAR2 is a key component of intestinal immunity.
This has been possible through the use of FFAR2 markers for antibody production. High-affinity primary antibodies have an affinity for the epitope in the fixed tissue. The high affinity of the antibody can cause problems during elution. High-affinity antibodies might be useful for studies on a single target. In this case, the FFAR2 markers could be used in lieu of an antigen.
To investigate the mechanism of binding, the bimolecular luminescence complementation assay and coimmunoprecipitation methods were used. In the latter, it was found that siRNAs of b–arrestin1 and siRNAs of b–arrestin2 significantly decreased the replication rates for H5N1 virus in A549 cell lines, respectively. It appears that siRNA b-arrestin1 has a higher affinity for H5N1 virus replication than siRNA b-arrestin2.
FFAR2 is a key cofactor in the endocytosis. It functions in conjunction with b-arrestin1 and clathrin during endocytosis. It is an excellent target of immunotherapy. High-affinity antibodies using the FFAR2 marker were designed to target IAV in a variety of tumors, including tumor cells, blood cells, and the liver.
Interferon Gamma is a key signal proinflammatory cytokine that plays an important role in the progression and pathogenesis many diseases. It is therefore considered an important marker to identify disease-specific immune responses. A singe-chain variable antibody (scFv), that targets FFAR2 is yet to be reported. This suggests it is not a reliable mark. However, it can be produced using recombinant Anti-Peptide Antibody Libraries.
Optimizing an ELISA requires a variety of choices, starting with the sample preparation and ending with the blocking buffer. If you don't know how to optimize your ELISA experiment, refer to our Boster Bio optimization guidelines. These guides will walk you through each step of the process and answer any questions you may have. These resources will make it possible to optimize your experiments within a fraction time.
Steve Boster's greatest use of the FFAR2 genes is to track his glucose levels. This gene enables researchers to identify which people have higher levels of blood glucose. It can also be used to monitor dietary intake. The FFAR2 gene is a highly sensitive marker for diabetes. In this study, a researcher identified those with high blood glucose levels as a potential danger for diabetes.
PMID: 9344866 by Sawzdargo M., et al. A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1.
PMID: 12684041 by Nilsson N.E., et al. Identification of a free fatty acid receptor, FFA2R, expressed on leukocytes and activated by short-chain fatty acids.