This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Gastric inhibitory polypeptide receptor.
This is a receptor for GIP.
The activity of this receptor is mediated by G proteins which activate adenylyl cyclase..
Human | |
---|---|
Gene Name: | GIPR |
Uniprot: | P48546 |
Entrez: | 2696 |
Belongs to: |
---|
G-protein coupled receptor 2 family |
gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2
Mass (kDA):
53.157 kDA
Human | |
---|---|
Location: | 19q13.32 |
Sequence: | 19; NC_000019.10 (45668187..45683722) |
Cell membrane; Multi-pass membrane protein.
In this article, you'll learn about the GIPR marker and Steven Boster. The best uses of the GIPR marker in clinical applications will also be covered. We will also touch on high-affinity prima antibodies and HUVEC. Let's get started. GIPR is an important marker for research in the field of gastrointestinal diseases. This marker can be expressed by the gut bacteria.
Researchers have used a GIPR based gene expression assay to identify antibodies that are highly specific for GIP. GIP is a gene that expresses a variety protein, including phospholipases. The GIPR gene binds a variety of GTPases, including glycoproteins, histamine, and serine. GIP is highly conserved in mammals with seven of the eight residues being shared between mouse and rats sequences. High-affinity antibodies that use the GIPR marker can be useful in animal toxicology or pharmacology.
GIP binds the ECD via an a-helical region located between residues 12-30. This interaction leads to cAMP formation. The GIPR Fab possesses high affinity for both free and cell-bound GIPr. This specificity makes it an excellent choice for the detection of many human hormone receptors.
This study revealed that high-affinity IgM could be isolated from mice with or without the IgD mutation. The generation of protective high affinity IgM in IgD-deficient mice was delayed. This GIPR-based antibody assays have been used in animal studies for decades. These results are a significant step forward in the field of research on autoimmune diabetes. These antibodies allow us to develop novel antigens that can be used to treat a variety of inflammatory diseases.
Abcam scientists can also measure affinity using the GIPR marker. These data can be found on the product pages. These graphs show three independent experiments. The process can be repeated multiple times until an antibody is found. If the affinity has been lost, the antigen will still be active in the target.
One population-based study showed that the prevalence of obesity was related to the GIPR Polymorphism. This result suggests that the gene is involved in the storage of fat. Due to the conflicting results, further studies are needed. Obesity has been a significant public health problem in developed nations. Genetic and environmental factors play a role in its development. Twin studies have shown up to 80 percent variance in body mass is genetic. Genetic contributions are most prominent in severe obesity and early-onset. These studies highlight the need to conduct additional research to learn more about the mechanisms of obesity, and the role that GIPR plays in this process.
GIPR expression is largely determined by transcript levels, and functional validation of the gene on GIP-induced Ca2+ influx and cAMP levels in macrophages and microglia is difficult to achieve. Additionally, Gipr expression can be limited by the low number of type 2 immune cell types compared to type 1. Reporter mice are required to label receptors. Transgenic mice are now available that allow for the deletion of conditional genes in large populations of immune cells.
Landesarztekammer Bavaria approved the KORA study. They used tagging sNPs. The study used two linkage blocks to determine the disequilibrium blocks. They were the coding region, and the 5’ region of the GIPR. It is possible to genotype the GIPR gene to identify high-risk diabetic patients. If the gene is found to have been mutated in either one or both parents, it could indicate a genetic condition that causes diabetes.
Boster Bio: Best Uses of The GPR Antibody
The ECV 304 GIPR marker has several potential applications. In vitro studies have shown the ECV 304 cell-line has different splicing patterns to other ECs. Moreover, ECs from different tissues exhibit different responses to GIP stimulation. This response was measured by increasing intracellular calcium levels. The HUVEC produced more ET-1 and endothelin-1.
ECV304 is a spontaneously transformed line that was derived from a Japanese HUVEC cell culture. This cell line was considered a useful biomedical research tool due to its unique ability to demonstrate angiogenesis in vitro and G protein-coupled receptor signal transduction. Recent reports have shown that ECV304, T24/83, and T24/83 share similarities in gene expression. Researchers were able to compare the functions in human endothelial cells using both cell lines.
Researchers have identified common molecular pathways that are involved in the onset or progression of infection by using this gene as a model system. ECV304 cells infected can show changes in mRNA levels that can predict the risk for cardiovascular diseases during the perinatal time. Microarray profiling identifies genes that are associated with these diseases and can also be used to determine their gene expression.
The ECV 304 GIPR marker is also useful in cancer research. Similar permeability to R123 was seen in immortalized cell lines, bEnd3, and ECV304. However, both cell lines failed to achieve sufficient paracellular tightness when compared to the in vitro BBB permeability model. Efforts to enhance paracellular tightness in both cell lines are required to mimic the BBB tight barrier in vivo.
PMID: 7589426 by Volz A., et al. Molecular cloning, functional expression, and signal transduction of the GIP-receptor cloned from a human insulinoma.
PMID: 7556958 by Gremlich S., et al. Cloning, functional expression, and chromosomal localization of the human pancreatic islet glucose-dependent insulinotropic polypeptide receptor.