Geminin Antibodies

AND GMNN ELISA kits, Geminin Proteins

Many biological assays use antibodies to detect Geminin, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Geminin in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Geminin antibodies...

We validate the specificity of these antibodies to Geminin by testing them on tissues known to express GMNN positively and negatively. Browse below to find the GMNN antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Geminin? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the GMNN Western Blot Guide

GMNN Infographic by Boster Bio

All GMNN Products

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Specificity

Applications

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Anti-Geminin/GMNN Antibody Picoband®

Western blot analysis of Geminin/GMNN using anti-Geminin/GMNN antibody (A06060-3)

IHC analysis of Geminin/GMNN using anti-Geminin/GMNN antibody (A06060-3)

Human, Mouse

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A06060-3
100 μg/vial

Anti-Geminin Monoclonal Antibody

Western blot analysis of Geminin expression in HeLa cell lysate

Human

IF, IHC, ICC, WB

$370
Cat # M06060
100 μl

Anti-Geminin GMNN Antibody

Western blot analysis of HeLa, using GMNN antibody at 1:1990 dilution

Immunohistochemistry of paraffin-embedded rat brain using GMNN Rabbit pAb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human colon carcinoma using GMNN Rabbit pAb at dilution of 1:100

Human, Mouse, Rat

IHC, WB

$405
Cat # A06060-2
100ul

Geminin (GMNN) (NM_015895) Human Over-expression Lysate

Human

$628
Cat # LS051053
20 µg, 100 µg

Geminin (GMNN) (NM_015895) Human Recombinant Protein

Coomassie blue staining of purified GMNN protein

$1062
Cat # PROTO75496
20 µg

GMNN Overview

Facts about Geminin.

GMNN 3D structure. Source: alphafold

Geminin (GMNN)

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complicated (pre-RC). It is degraded during the mitotic phase of the cell cycle.

Its destruction at the metaphase-anaphase transition allows replication in the achievement cell cycle. .

Gene Information

Human
Gene Name:	GMNN
Uniprot:	O75496
Entrez:	51053

Family

Belongs to:

geminin family

Alternative Names

Gem; geminin; geminin, DNA replication inhibitor; RP3-369A17.3

Molecular Weight

Mass (kDA):
23.565 kDA

Genomic Context


Human

Location:	6p22.3
Sequence:	6; NC_000006.12 (24774937..24786099)

Subcellular Localizations

Cytoplasm. Nucleus. Mainly cytoplasmic but can be relocalized to the nucleus.

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Malignant Paraganglionic Neoplasm
Nervousness
Malignant Neoplasm Of Breast
Genomic Instability
Cell Transformation, Neoplastic
Hyperplasia
Neoplasm Metastasis
Aneuploidy
Mammary Neoplasms

Cell Invasion
Adenoma
Neoplasm Invasiveness
Endoreduplication
Colonic Neoplasms
Carcinogenesis
Malignant Squamous Cell Neoplasm

Interacting Proteins

CDT1
HOXC9
MCIDAS
ZNF439

Pathways

Cell Cycle
Dna Replication
Mitosis
S Phase
Cell Proliferation
G1 Phase
Proteolysis
Cell Division
Dna Replication Initiation
Metaphase
Localization
Regulation Of Dna Replication
Chromatin Remodeling

Cell Death
G2 Phase
Neurogenesis
M Phase
Cell Differentiation
Pathogenesis
Anaphase

PTMs

Phosphorylation
Ubiquitination
Cleavage

Acetylation
Methylation
Carboxylation

Dephosphorylation

Research Areas

Breast Cancer
Cell Cycle and Replication
Core ESC-Like Genes
DNA Repair
DNA replication, Transcription, Translation and Splicing

Nuclear Receptors, Coactivators and Corepressors
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GMNN

References

PMID: 9635433 by McGarry T.J., et al. Geminin, an inhibitor of DNA replication, is degraded during mitosis.

PMID: 14993212 by Sugimoto N., et al. Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding.