This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
GRK5 Antibodies
AND GRK5 ELISA kits, GRK5 Proteins
Many biological assays use antibodies to detect GRK5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with GRK5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop GRK5 antibodies...
Designing a Western blot for GRK5? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GRK5 Western Blot GuideDesigning an IHC experiment for GRK5? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the GRK5 IHC GuideGRK5 Infographic by Boster Bio
All GRK5 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA
$480
Cat # EKC1709
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1709
1 kit, containing one 96-well plate and all necessary reagents
GRK5 Overview
Facts about G protein-coupled receptor kinase 5.
G protein-coupled receptor kinase 5 (GRK5)
Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation.
Phosphorylates an assortment of GPCRs, such as adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1.
Gene Information
| Human | |
|---|---|
| Gene Name: | GRK5 |
| Uniprot: | P34947 |
| Entrez: | 2869 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
EC 2.7.11; EC 2.7.11.16; FLJ39780; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; GPRK5FP2025; GRK5
Molecular Weight
Mass (kDA):
67.787 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 10q26.11 |
| Sequence: | 10; NC_000010.11 (119207571..119459745) |
Tissue Specificity
Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
Subcellular Localizations
Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.
Diseases
- Heart Failure
- Hypertensive Disease
- Hypertrophy
- Neoplasms
- Parkinson Disease
- Alzheimer's Disease
- Malignant Neoplasms
- Inflammation
- Cardiac Hypertrophy
- Myocardial Ischemia
- Infarction
- Myocardial Infarction
- Diabetes Mellitus
Interacting Proteins
- Htr4
- NFKBIA
Pathways
- Pathogenesis
- Localization
- Receptor Internalization
- Endocytosis
- Rna Interference
- Receptor Recycling
- Cell Proliferation
- Inflammatory Response
- Bioluminescence
- Prenylation
- Hyperphosphorylation
- Aging
- Chemotaxis
PTMs
- Phosphorylation
- Prenylation
- Palmitoylation
- Reduction
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GRK5
bosterbio.com/bosterbio-gene-info-cards/GRK5
References
PMID: 7685906 by Kunapuli P., et al. Cloning and expression of GRK5: a member of the G protein-coupled receptor kinase family.
PMID: 8381058 by Ungerer M., et al. Altered expression of beta-adrenergic receptor kinase and beta 1- adrenergic receptors in the failing human heart.