Homeobox protein Hox-C13 Antibodies

Homeobox protein Hox-C13 (HOXC13)

Transcription factor which plays a role in hair follicle differentiation. Regulates FOXQ1 expression and that of other hair-specific genes (By similarity).

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Gene Information

Human
Gene Name:	HOXC13
Uniprot:	P31276
Entrez:	3229

Family

Belongs to:
Abd-B homeobox family

Alternative Names

homeo box 3G; homeo box C13; homeobox C13; Homeobox protein Hox-3G; homeobox protein Hox-C13; HOX3; HOX3GNUP98/HOXC13

Molecular Weight

Mass (kDA):
35.379 kDA

Genomic Context

Human
Location:	12q13.13
Sequence:	12; NC_000012.12 (53938831..53946544)

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Leukemia
• Dysplasia
• Myeloid Leukemia
• Malignant Paraganglionic Neoplasm
• Pilomatrixoma
• Leukemia, Myelocytic, Acute
• Skin Neoplasms
• Hair Diseases
• Disease Of Adrenal Medulla
• Dystrophia Unguium
• Chimera Disorder

• Hematologic Neoplasms
• Ameloblastoma
• Dystrophy
• Chromosomal Translocation
• Keratocystic Odontogenic Tumor
• Ectodermal Dysplasia
• Odontogenic Tumors

Interacting Proteins

• ELF1
• NFATC1

Pathways

• Dna Replication
• Regeneration
• Cell Cycle
• Anagen
• Catagen
• Cell Differentiation
• Cell Proliferation
• Telogen
• Hair Follicle Morphogenesis
• Hair Follicle Development
• Gene Silencing
• Fertilization
• Proteolysis

• Negative Regulation Of Cell Proliferation
• Keratinocyte Differentiation
• Nail Development
• Reverse Transcription
• Cell-cell Adhesion
• Gastrulation
• Cornification

PTMs

• Cleavage

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HOXC13

Boster Bio - Best Uses Of The HOXC13 Marker

The HOXC13 gene marker offers many advantages in research. It has the potential for being extremely precise in determining a target’s DNA sequence. Using a Boster antibody will greatly improve your chances of finding a target that is truly unique. The HOXC13 antibody is available in high affinity primary antibodies, oligonucleotides and supershifted compounds.

HOXC13 binding to distinct TAAT/TTAT core motifs

This study shows that HOXC13 binds differently to TAAT and TTAT sequences. However, not all T(A/T]AT core motifs. This suggests that HOXC13 can not be activated universally by TAAT and TTAT motifs. This finding suggests that more studies will be needed to determine the mechanism of HOXC13's repression.

Multiple motif discovery allows for the identification of all binding motifs that can be found in the same TF/co-factor. The motifs and mask sequences modes are implemented by ChIPMunk. In the first mode, the strongest motif is identified. In the latter mode, the second motif in sequences containing the first motif are identified. In the latter, the motif is preferentially bound.

Homeodomain proteins are able to distinguish between A-T or T-A base pairs within a DNA Recognition Core. The TAAT and TTAT core motifs are a consensus of motifs that are highly conserved across species. Functional specificity of homeodomains was mapped to amino-acid sequences within the conserved parts of the homeodomain.

HOX TFs induce distinct gene expression patterns during spinal cord differentiation in vitro. The posterior HOXTFs also increase transcriptional diversity, which enhances gene expression in the spine cord. Ultimately, HOX protein are crucial in the development and differentiation the spinal cord. Hox proteins need to be examined in vivo to confirm their transcriptional specificity.

Activation of HOXC13 genes is controlled by the TAAT/TTAT central motif. The TAAT core motif is also found in the 200-bp proximal region of hHa5/hHa2 gene genes. Moreover HOXC13 negatively correlated in these genes' expression.

HOXC13 also belongs to the Eph family. It binds to the promoter of EphB4 gene, which regulates endometrial cell migration and tube formation. It also regulates gene transcription of subunits b3integrin. This discovery may have implications in disease prevention and management.

HOXC13 supershifted complicatedes

HOXC13 codes for the HOXC13 genetic gene. This marker is used in a variety of biological processes. Boster Bio provides a variety of products to support research, including gene therapies, diagnostic testing, as well as drug discovery. This article explains the most effective uses of HOXC13. We hope you find this useful. You can also download the complete guide. It is based on scientific reviews and published data.

HOXC13 binds distinct core recognition motifs to regulate the expression of hair keratin proteins. We cloned the sequences to the pNassb-reporter vector to investigate the binding characteristics of HOXC13. The HOXC13 gene promoters were analyzed using EcoRI-site forward and XhoI-site reverse primers.

Molecular analysis has shown that HOXC13 binding motifs could have an unintended effect on KAP genes. This HOXC13 binding motif coincides with the keratin molecule, which strongly suggests a gene-activating role during early trichocyte differentiation. The consensus sequence for Meis1 binding is not present in the HOXC13 binding complex. The proximal 0.3a2 segment is located 27 nucleotides downstream of a nonbinding TAAT motif.

It is important that research into aging targets the HOXC13 genetic gene. The study involved ABI2, ATM BCL11A, BCL11A, BTG1 and BTG1. AASS also predicted the regulation more than 376 genes. These genes are vital for the development of new technologies or drugs. The best uses of HOXC13 geneexpression marker genes include cancer research as well as identifying the causes and consequences of aging.

High-affinity primary antibodies

Next-generation sequencing (NGS), has revolutionized the study and analysis of antibody repertoires. Single-cell sequencing allows for large-scale determinations of paired L/H chains. This provides the genetic record of evolutionary events. It is now possible to use computational tools to reconstruct antibody lineages. Affinity-matured antibodies have crystal structures that provide new insights into the affinity maturation process.

Affinity-matured antibodies were first identified by their affinity towards a protein antigen rather than a hapten. The antibodies specific to hen egg white lysozyme represented various stages of affinity maturation. A higher affinity was associated with more somatic mutations. Interestingly, increased affinity was not related with the formation or increase in total surface area or hydrogen bonds. Instead, affinity maturation was accompanied with improved shape complementarity at VH-HEL interface.

To produce high-affinity primary antibodies by using the HOxC13 HOxC13 marker we first produced and characterized pFLAG HOXB13. This contains all the PSA promoter. We then grew LNCaP cell cultures under 5% CDT-FBS for three days. After three days of growth, we added 10nM to the cells. Finally, we collected nuclear extracts following the procedure described in Lee et.al. 2003. Pre-incubated the nuclear extracts with 4 ug Anti-HOXB13 Antibodies for 3 Hours. The proteins were separated using 10% SDS–PAGE. Finally, the eluates were transferred to a PVDF membrane. Finally, we immunoblotted the proteins using horseradish peroxidase-conjugated secondary antibodies.

With the help of next-generation sequencing, antibodies can be detected with high confidence. This method can also be used for the reconstruction of antibody lineages and the inference of germ-line progenitor sequences. However, the original sequences of the VLJL and VHDJH junction are uncertain. Affinity maturation sequences are not a reliable source of information for identifying insertions and deletions.

Alternativly, mass spectrometry can be combined with hydrogen/deuterium Exchange to detect antibodies more precisely. This technique allows for the detection of local protein dynamics, as well deuterium uptake within the backboneamide. An antibody that recognizes both HIV-1 Env or non-HIV Antigens can detect dynamic regions in proteins.

HOXC13 oligonucleotides

Boster Bio HOXC13 oligonucleotides have six HOXC13 binding proteins. These sequences are critical for regulating HOXC13 activitiy. HOXC13 is overexpressed in tumor cells, which causes them to form three-dimensional (3D), colonies in soft agar. A novel drug targeting HOXC13 could be a promising therapy for cancer because it contributes to tumorigenesis.

HOXC13 plays an important role in cell growth and regulation. Knockdown HOXC13 promotes cell death in SW480 cells. Boster Bio provides HOXC13 oligonucleotides to allow you to study HOXC13 vivo. Here's how it works.

HOXC13 binds with core motifs of the hHa5 Promoter. It is more apt to the core motif than the TATA-box. Most non-binding HOXC13 motifs can be found with at least two or more deviations from their consensus sequence. One deviation from this motif would abolish their HOXC13 bindability.

HOXC13 Antisense decreased cell population at G0/G1 as well as S phases. The number apoptotic cells increased by comparison to the control cell population. The G0/G1 cellular population was reduced from 63.3%, to 32.3% by 0.75 mg/mL. Moreover, HOXC13 oligonucleotides of 1.25 and 1.75 ug/mL boosted the percentages of apoptotic cells.

Clontech's HOXC13 oligonuclideDNA fragmentation kit was used. It was made with a cell line expressing DpHOXC13 oligonucleotides or scramble antisense oligonucleotides. The cells were then harvested after incubating them for 25 min with 4% formaldehyde.