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- Table of Contents
Facts about Lysine-specific histone demethylase 1A.
Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. May play a role in the repression of neuronal genes.
Human | |
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Gene Name: | KDM1A |
Uniprot: | O60341 |
Entrez: | 23028 |
Belongs to: |
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flavin monoamine oxidase family |
amine oxidase (flavin containing) domain 2; AOF2; AOF2lysine-specific histone demethylase 1; BHC110; BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; KIAA0601KDM1; LSD1; LSD1BRAF35-HDAC complex protein BHC110; lysine (K)-specific demethylase 1; Lysine (K)specific Demethylase 1A; Lysine (K)-specific Demethylase 1A; lysine-specific histone demethylase 1A
Mass (kDA):
92.903 kDA
Human | |
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Location: | 1p36.12 |
Sequence: | 1; NC_000001.11 (23019443..23083691) |
Ubiquitously expressed.
Nucleus.
You've found the right place if you are interested to learn more about the KDM1A Gene. Boster is a reputable company that offers high-affinity primary antibodies that have been validated in a variety of techniques, including Western Blotting, Immunohistochemistry, and ELISA. Boster antibodies have been extensively cited over the past 25 year in scientific journals. This gives you confidence that the antibody that you order is the best quality.
KDM1A is a good place for you to start if you're looking for a chromosomal marker that identifies a gene of your interest. This gene is related with other genes such as ACTN3, SNAI, and ABCA7. In fact, KDM1A is also related with genes such as spectrin repeats-containing the nuclear envelope protein 1.
The KDM1A Gene has many functions, including regulating hormones. The protein can also be found on chromosome 1. The gene can be transcribed into many splice variants. It has many applications, such as neurodegeneration with iron accumulation in the brain, smith-lemlii-opitz Syndrome, and dystonia.
This gene is responsible to the development and maintenance the p60 subunit catalase enzyme. It is also responsible for the development of potocki–lupski syndrome (pwls). Richieri-costa syndrome is also linked to the KDM1A gene. However, if you want to find out more about the KDM1A gene, you'll need to conduct a clinical trial.
To profile a GTF2I population, the researchers used iPSC-lines. This allowed them to identify 1554 genes with a GTF2I binding site. They focused on the peaks that were conserved across all samples, and then considered 436 core GTF2I targets. The results also showed enrichment of disease categories. Further, the KDM1A gene was compared with genes known to play a role in the development the disease.
The inhibition by H2O2 of the enzyme is required for the activity-dependent neuroprotector to be synthesized from the KDM2A markers. This catalysis results in H2O2. This enzyme is controlled by the thiol disulfide switch. The ratio of TCEP beads to the recombinant KDM1A proteins was two to one. The protein was then subjected at a tryptic acid and analyzed mass spectrometry.
KDM1A's crystal structure showed that Cys-618 was proximal Cys-608 in MAO. MAO/B does not have the Cys-618 residue. Due to competition with the enzyme substrate, MAO-A/B's thiol-labeling (20) was significantly reduced. TCP doesn't compete with RN1 inactivation. Further research on the effects of FAD-directed inhibitors on KDM1A is needed.
H2O2 generates multiple roles in the biochemistry of the cell. RN1 can be used to block H2O2 production and inhibit activity-dependent neuroprotectors. KDM1A can also bind with multiple targets in nucleus like p53-rich neurons. Thus, recombinant KDM1A is a potent neuroprotector that can be used in clinical trials.
PELP1 plays a significant role in E2-mediated brain neuroprotection. It also plays a key role in rapid extranuclear communication, mediates cognitive function, induces survival signaling, and other important roles. PELP1 is therefore essential for optimal E2-mediated functions of the brain. But what does PELP1 actually do? The following analysis will help you understand what PELP1 does.
We have shown that KDM1A activity is inhibited using H2O2. H2O2 pretreatment is particularly effective in inhibiting KDM1A activity, blocking the binding of Biotin Mal to Cys-618. This suggests that Cys-618 regulates KDM1A’s activity through intramolecular disulfide-bond formation. It also abuts the conserved Rossmann fold. Cross-strand disulfide bond creation may regulate the FAD accessibility as well as the redox poteniy.
PCR assays were carried out using a recombinant KDM1A-protein. After desalting, the recombinant KDM1A proteins was subject to pre-treatment with 25mm RN1 or vehicle controls. After 10 minutes, the sample were analysed with the biotinylated protien and the total KDM1A mark. ImageJ was used as a tool to quantify the band intensities.
Boster Bio offers a wide range of quality products for laboratory workers who value the quality and safety of their antibody reagents. Their antibodies have been validated for high affinities on a variety of platforms, including Western Blotting and Immunohistochemistry. This product can be used to optimize your assays. The antibodies used in this process are specific to the length of the immunogen, so the actual working concentration will depend on the end user's method.
There may be significant differences in the ChIP-seq data from different laboratories. Background signals can increase due to chromatin fragmentation, unrecognized antibodies cross reactivity, and other factors. Background signals may also be caused by variation in sequencing efficiency. Good sensitivity and specificity are required for ChIP-seq data verification. CST tests antibodies for a wide variety of epigenetic processes including gene expression, cancer development, and aging.
Different ChIPseq experiments show different patterns for peaks. Some ChIP-seq experiments have sharply defined peak patterns, while others have wider smears. There are many algorithms that can interpret and validate these peaks. These algorithms allow researchers evaluate the quantitative peaks of ChIP/seq data and determine whether they're real-time or filtered.
Clonal antibodies are also recommended if possible. This increases confidence in true positives. Antibodies should be tested by qPCR on multiple genomic loci to determine their specificity. The antibodies must have at least fivefold enrichment to do this. These antibodies should be used together with other genomic loci to ensure accurate ChIP-seq data.
The quality of the used antibodies is a major factor in the quality of ChIP-seq data. High sensitivity and specificity is required to detect enrichment peaks in the absence of background noise. Although many commercial antibodies have been tested in ChIP studies for their ability to detect enrichment peaks, not all of these antibodies are suitable for the analysis of genome-wide protein and DNA interactions. Some antibodies are not suitable only for locus-specific enrichment.
Alignment to the reference gene is the first step to analyze millions of reads. The SEQanswers SEQwiki hosts common tools to perform ChIP-seq analysis. Bowtie and ELAND are the most popular alignment algorithms. MAQ is also a popular one. Different alignment algorithms can trade speed for accuracy and precision. Moreover, different algorithms align to repetitive regions of the genome.
The ChIP-seq data generated by the method are highly susceptible to artifacts and biases. CHANCE is an excellent tool to evaluate the quality of ChIPSeq data. It can identify two types. It can detect problems in the sequencing process, such as a base-call bias. The second type, read density bias (which can occur at transcription exon boundaries and transcription start sites), is also possible. This bias can also happen in a cell-dependent fashion.
PMID: 12032298 by Hakimi M.-A., et al. A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes.
PMID: 11102443 by Humphrey G.W., et al. Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.