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- Table of Contents
Facts about Keratin, type I cytoskeletal 23.
Human | |
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Gene Name: | KRT23 |
Uniprot: | Q9C075 |
Entrez: | 25984 |
Belongs to: |
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intermediate filament family |
CK23; CK-23; cytokeratin 23; cytokeratin-23; DKFZP434G032; HAIK1; hyperacetylation-inducible type I keratin; K23histone deacetylase inducible keratin 23; keratin 23 (histone deacetylase inducible); keratin, type I cytoskeletal 23; keratin-23; MGC26158; type I intermediate filament cytokeratin
Mass (kDA):
48.131 kDA
Human | |
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Location: | 17q21.2 |
Sequence: | 17; NC_000017.11 (40922696..40937646, complement) |
Boster Bio can provide anti-KRT23 antibodies for scientists looking for the best products that will test their experiments. You will get a gift card as well as product credits as a reward for your review. This is a fantastic way to recognize your innovative efforts. To learn more about Boster Bio and its anti-KRT23 primary antibodies, read on.
Boster Bio is your source for anti-K1C23KRT23 antibodies. This anti K1C23 primate antibody reacts with Human K1C23. Available in liquid form, this antibody is a synthetic peptide derived from the human K1C23 protein. Blocking peptides can also be purchased at a variety prices depending on their length.
The KRT23 gene is involved in regulating the OC cell line EMT pathway. Based on bioinformatics analysis, it is possible that KRT23 may accelerate EMT. KRT23 depletion led to a reduction in the expression levels of p–Smad2 and of p–Smad3. Both genes are members of the TGF-b/Smad signaling pathway. They play a crucial role in cancer progression as well as in regulating the cell's growth and migration.
Single-cell western blotting is a powerful technique for quantifying protein expression in individual cells. To do this, 2000 cells were separated and probed using fluorescently labeled antibody. This allows cells to be differentiated into three different cell types and can be used downstream of scRNAseq analyses as a validation tool. This technique can be used to investigate inflammatory processes.
The KRT23 gene first was discovered in OC tissues and cell lines. It was found to be overexpressed in these cells and was implicated in the regulation of OC cell migration. Knockdown KRT23 prevented OC cells from invading and migrating, indicating a possible role for this gene within the TGFb/Smad signaling pathway. Further research is required to determine the in vivo role of KRT23. The authors declare that they have no financial or other conflict of interest.
JTSBIO Co., Ltd., Wuhan, China, developed three siRNAs specifically to inhibit KRT23. They used the sequence of nonsense RNAi 5-UUCCCGGGAAGATT-3. To transfect cells the siRNAs of the three were combined with LipofectamineTM to deliver them into specific cell lines. The siRNA oligos were detected by western blotting and RTQPCR after transfection. The siRNAs were highly effective in knocking down KRT23.
In vitro studies have shown KRT23 expression is affected by liver disease severity. Moreover KRT23 expression was also detected in two human OC-cell lines, SKOV3 & HOSEpiC. KRT23 in vitro research was conducted on SKOV3. In vitro experiments proved that KRT23 can still be induced in single cells by HCV infection.
HCV-infected, 2'CMA-treated cells showed high levels of KRT23 proteins. The protein assays indicated that the 48 kDa isoform was preferentially expressed. These findings indicate that HCV-mediated KRT23 level changes may be due to the liver’s regenerative capabilities. Further, PPARa-deficient mice also have increased KRT23 protein levels.
Anne Gao is the Summer 2021 Innovating Scientists Rewards in Boston Bio. This is a brand new award program. Anne is an undergraduate student of Purdue University. She will use the scholarship for further studies in the sciences. Boster Bio is an organization that supports the research of engineers and scientists in all fields. She hopes to use the award for biomedical research, and to help others become successful.
PMID: 11135429 by Zhang J.-S., et al. Keratin 23 (K23), a novel acidic keratin, is highly induced by histone deacetylase inhibitors during differentiation of pancreatic cancer cells.