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- Table of Contents
Facts about U6 snRNA-associated Sm-like protein LSm2.
Binds specifically to the 3'-terminal U-tract of U6 snRNA.
May be involved in pre-mRNA splicing (By similarity).. .
Mouse | |
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Gene Name: | Lsm2 |
Uniprot: | O35900 |
Entrez: | 27756 |
Belongs to: |
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snRNP Sm proteins family |
C6orf28chromosome 6 open reading frame 28; G7b; LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae); Protein G7b; Small nuclear ribonuclear protein D homolog; snRNP core Sm-like protein Sm-x5; snRNP; U6 snRNA-associated Sm-like protein LSm2; YBL026W
Mass (kDA):
10.835 kDA
Mouse | |
---|---|
Location: | 17 B1|17 18.52 cM |
Sequence: | 17; |
The LSM2 Marker is a high-affinity protein that has many uses in molecular biology. LSM2 is found in a variety cell types including neurons, cancerous cells, and glial cells. LSM2 is also present on many proteins of the immune systems, including monocytes. Boster's high-affinity antibodies have been validated on immunohistochemistry, Western Blotting, and ELISA.
Boster Bio LSM2 probes detect PspA (a plasmid encoded protein) within S. pneumoniae. The probes were tested for specificity against the PspA gene using PCT products and partially digested, plasmid-encoded inserts. Both Southern hybridization and agarose gel electrophoresis were performed to ensure that the clones contained the correct inserts. After cloning was complete, plasmids were digested in BamHI and then transferred to a nylon membrane.
Table 1 shows the sequences of both probes. Figure 16 displays the pspA probe, and the full length Rxl-pspA pspA probe. Both probes are used to amplify parts of the pspC gene. The Table also contains the nucleotide sequences for the pZero vector probe and the C588 probe. These results show that the Boster bio LSM2 probes as well as primers are complementary.
The structure of LSM2-8's ring is now understood. In vivo structure function analysis of the LSM2-8 is comparable to the Sm RING of yeast. Its Alanine scanning revealed one lethal mutant and one nonlethal mutation, both involving amino acids that bind UUU trinucleotide. Another study 235 pairwise combinations Lsm mutations revealed six inter-Lsm synthetic lethalities, and 45 non-lethal growth defects.
The LSM2-8 is a heteroheptamer. This ring may be formed by the proteins of heteroheptameric ring. This ring acts as a buffer to the Lsm subunits. It is not clear how Lsm2-Lsm8 works in vivo. Also, how it can possibly be used in diagnostic testing.
The LSM2-plasmid (H36A–N38A), was used to convert lsmD strains into 2u-U6 LSM2 cells. The transformed strains could grow at low temperatures, but they failed to grow on YPD-agar at 30degC to 37degC. Its LSM2-F35A–N37A strains grew well with low temperatures, and slowly at high temperature.
When Lsm2-8 interacts with U6 snRNA, a yeast Lsm2-8 protein abets its function. The Lsm2-8 Complex compensates for the U6 missing snRNA. This makes the LSm2-8 RNA probe an excellent choice. In addition, Lsm2-8 can be used in research to determine how the LSM2-associated Lsm2-8 protein affects the U6 snRNA.
Synthetic lethality is achieved by double deletion of LSM genes that are not essential. FOA also prevents growth at 37 degrees Celsius. In addition, the LSM2-based plasmids can complement the five lethal lsmD alleles. These LSM-based plasmids require the LSM ring to function. The LSM ring could also be disrupted if another subunit is overexpressed.
As the name suggests, high-affinity prima antibodies are raised against a particular antigen. They are useful for detecting antigens and purifying them. They allow researchers the ability to analyze living cell components at a molecular level. There are several advantages of using such antibodies for research. They have been validated in multiple research applications using samples from different species. Second, they have been proven in knockout and protein array validations.
In order to measure affinity, scientists need to know how to measure KD. It is a measurement of the binding affinity between monoclonal antibodies (and their ligand). Bimolecular reactions use the equilibrium dissociation coefficient (KD) to measure affinity. Asymmetric binding occurs. The KD values are proportional to both reactants. Therefore, the KD value of a reactant is proportional to its concentration, so the KD value will determine the affinity.
High-affinity prima antibodies using the LSM2-marker are not only highly specific but can also be used for multiple antigens analysis in one sample. Researchers can identify up to 61 antigens from a single tissue by using a combination of these methods. Its use in research has led the development of complicated digital analytical tools. These tools are also known as image Cytometry.
There are many commercial sources of high-affinity primary antibody. However, these antibodies are not all the same quality. This can make a difference in the outcome of your experiment. You have two options. You can either conduct your experiments yourself or consult the literature to verify the quality. You can also purchase them directly from the manufacturer if you are unable.
This study describes PCR amplification of psgA-like sequences of pneumococcal DNA and its use in the diagnosis of invasive pneumococcal disease. The PCR product contained two unique restriction enzymes, EcoRI, and NdeI. It was then ligated to the pET28a gene vector. His-tagged pneumococcal skin protein A (PspA31-189) was expressed in E. coli strain BL21. The His-Bind Resin column was used to purify the product.
The full-length pspA DNA probe was used for detection. The pspA-like sequence was also detected using a chemiluminescent-labeled pspA-like probe. To generate fluorescent signals from the probes, they were digested by BamHI (and Sail). A PCR cycle was used in order to amplify the pspA-like sequences bacterial genome in a single tube.
PCR-based amplification can distinguish between overlapping fragments or segments of the pspA gene. This method can identify pneumococcal spores. A single-tube cloning device can also produce a PspAlike sequence by using the PCR technique.
The cloned PspA fragments have been amplified using primers LSM4 or LSM6. This protocol also allows detection of PspAspecific antibodies. Antibodies against PspA were detected by using goat anti-human IgG-alkaline phosphate and a substrate against goat anti-human IgG-alpha-phosphatase. The measurement was 415 nm.
By PCR amplification, the C terminal two-thirds (C-terminal) of PspA was amplified to produce the truncated PspAlike sequence. After cloning in E.coli, the recombinant PspAs could be tested for cross-protection with variant capsular type. When challenged with parental strains of S. pneumoniae types 3 and 6A, the truncated PspA-like sequence was protected against early death.
PMID: 9271633 by Bedian V., et al. A gene belonging to the Sm family of snRNP core proteins maps within the mouse MHC.
PMID: 11797099 by van Kooij M., et al. Genotype versus phenotype: conflicting results in mapping a lung tumor susceptibility locus to the G7c recombination interval in the mouse MHC class III region.