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- Table of Contents
MEOX2 / Homeobox protein MOX-2 · Western blot design guide
Design a Western Blot for MEOX2
Source-linked MEOX2 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MEOX2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MEOX2 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~33.6 kDa | |
| Observed band | ~34 kDa | |
| Gel | 10% (catalog A05167-2) | |
| Positive control | Placenta (IHC candidate; verify WB) | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked MEOX2 Western Blot Protocol Options
The A05167-2 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human A549, rat heart, rat lung, mouse heart, mouse lung (catalog A05167-2) |
| Gel % | 10% (catalog A05167-2) |
| Load | 30 ug; reducing conditions (catalog A05167-2) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A05167-2) |
| Membrane | nitrocellulose membrane (catalog A05167-2) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A05167-2) |
| Primary antibody | A05167-2 · 0.5 μg/mL (catalog A05167-2) |
| Primary incubation | overnight at 4°C (catalog A05167-2) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A05167-2) |
| Secondary incubation | 1.5 hour at RT (catalog A05167-2) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A05167-2) |
| Detection | ECL (catalog A05167-2) |
Section 2
What Is the Expected MEOX2 Western Blot Band Size?
MEOX2 is predicted at 33.6 kDa and observed near 34 kDa; the cause of the small difference is not established.
| Single band near 34 kDa | Consistent with predicted MEOX2 mass of 33.6 kDa and the observed band near 34 kDa |
| Weak band near 34 kDa in whole-cell lysate | Nuclear MEOX2 may be diluted in the sample |
| No visible band in whole-cell lysate | Nuclear MEOX2 may be below detection in the sample |
| Band near 34 kDa in a nuclear fraction | Consistent with the nuclear location of MEOX2 |
💡Expected MEOX2 appearanceMEOX2 has a predicted mass of 33.6 kDa and an observed band near 34 kDa; confirm identity with an antibody specificity control.
| UniProt predicted mass of 33.6 kDa | Places the expected full-length band near 34 kDa |
| UniProt calculated mass of 33,594 Da | Corresponds to the observed band near 34 kDa |
| Predicted full-length molecular weight | Provides the reference size for assessing bands above or below 34 kDa |
| Predicted MEOX2 protein mass | Agrees closely with the observed band near 34 kDa |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Nuclear MEOX2 may be below detection in whole-cell lysate | Check a nuclear fraction and include a positive-control lysate |
| Band higher than expected | The supplied MEOX2 features do not explain a larger band | Verify its identity with MEOX2 depletion |
| Band lower than expected | Degradation or nonspecific binding may produce a smaller band | Use fresh lysate with protease inhibitors and verify identity with MEOX2 depletion |
| Multiple bands | The supplied features do not establish multiple resolvable MEOX2 forms | Compare each band with a MEOX2 depletion control |
| Weak or no signal | Nuclear MEOX2 may be diluted in whole-cell lysate | Check a nuclear fraction and a positive-control lysate |
Experiment evidence
Western blot images showing MEOX2 band
A05167-2 · MEOX2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MEOX2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MEOX2 in Western blot, you can use placenta tissue lysate.
Positive control: Placenta (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: MEOX2 is nuclear, so nuclear-enriched lysate may improve detection.
HPA tissue expression evidence for MEOX2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Placenta | endothelial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Not detected | Protein (IHC) | HPA → |
| Appendix | glandular cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Breast | adipocytes | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MEOX2 Western Blot Tips
Deeper troubleshooting and optimisation questions for MEOX2, answered from its protein features.
How should MEOX2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could an annotated isoform explain a second MEOX2 band?
Isoforms · UniProt lists one isoform and no alternative sequence. Its listed isoforms therefore do not explain a second band.
Do annotated modifications explain a MEOX2 band shift?
PTM · No modified residues or glycosylation sites are listed. These features provide no basis for assigning an extra band to a modification, and they do not rule out unannotated modifications.
Does this guide establish induction of MEOX2?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
How should transfer be checked for MEOX2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A05167-2 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MEOX2 be quantified?
Quantitation · Standard workflow guidance: quantify only a validated target band within the linear exposure range. Use consistent sample preparation and loading, retain biological replicates, and avoid interpreting saturation or loading differences as regulation.
Does the observed MEOX2 band match its predicted mass?
Interpretation · The reported band at about 34 kDa closely matches the predicted 33.6 kDa mass. The supplied features do not establish a band shift.
MEOX2 is annotated in the nucleus and nuclear speckles. A nuclear fraction is a reasonable choice when seeking its approximately 34 kDa band.
Use comparable nuclear preparations and loading for each sample, then quantify the band near 34 kDa consistently. Its nuclear localization makes fraction consistency relevant to the comparison.
No signal peptide or propeptide is listed, so the supplied features do not support assigning a smaller band to either cleavage event.
Use the approximately 34 kDa band as the expected position and check the identity of additional signals. The supplied isoform, cleavage, and modification annotations do not establish what those extra bands are.
MEOX2 is annotated in the nucleus and nuclear speckles. A nuclear fraction is a reasonable choice when seeking its approximately 34 kDa band.
Use comparable nuclear preparations and loading for each sample, then quantify the band near 34 kDa consistently. Its nuclear localization makes fraction consistency relevant to the comparison.
No signal peptide or propeptide is listed, so the supplied features do not support assigning a smaller band to either cleavage event.
Use the approximately 34 kDa band as the expected position and check the identity of additional signals. The supplied isoform, cleavage, and modification annotations do not establish what those extra bands are.
Boster reagents
MEOX2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-MEOX2 Antibody Picoband®
Cat # A05167-2
A05167-2 is a rabbit polyclonal anti-MEOX2 antibody listed for human, mouse, and rat. Its supplied WB image shows an approximately 34 kDa band in human A549 cells and rat and mouse heart and lung lysates. Independent validation is not supplied.
Which to pick: Only A05167-2 is listed. Its WB image provides a starting reference for human A549 cells and rat or mouse heart and lung lysates, using 0.5 μg/mL antibody under reducing conditions. Performance in other samples is not documented.
Source: BosterBio MEOX2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P50222.
- Human Protein Atlas. MEOX2 tissue expression.
- PMC9016080 — target-verified WB comparison
- PMC9099809 — target-verified WB comparison