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- Table of Contents
MGMT Antibodies
AND MGMT ELISA kits, MGMT Proteins
Many biological assays use antibodies to detect MGMT, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MGMT in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MGMT antibodies...
Designing a Western blot for MGMT? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the MGMT Western Blot GuideMGMT Infographic by Boster Bio
All MGMT Products
Product
Figures
Specificity
Applications
Price
MGMT Overview
Facts about Methylated-DNA--protein-cysteine methyltransferase.
Methylated-DNA--protein-cysteine methyltransferase (MGMT)
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme.
This is a suicide reaction: the enzyme is irreversibly inactivated. .
Gene Information
| Human | |
|---|---|
| Gene Name: | MGMT |
| Uniprot: | P16455 |
| Entrez: | 4255 |
Family
| Belongs to: |
|---|
| MGMT family |
Alternative Names
6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase
Molecular Weight
Mass (kDA):
21.646 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 10q26.3 |
| Sequence: | 10; NC_000010.11 (129467241..129770983) |
Subcellular Localizations
Nucleus.
Diseases
- Neoplasms
- Malignant Neoplasms
- Brain Neoplasms
- Glioblastoma
- Glioma
- Carcinoma
- Malignant Paraganglionic Neoplasm
- Carcinogenesis
- Colorectal Cancer
- Colorectal Neoplasms
- Astrocytoma
- Glioblastoma Multiforme
- Malignant Glioma
Pathways
- Methylation
- Dna Repair
- Dna Methylation
- Mismatch Repair
- Drug Resistance
- Cell Cycle
- Pathogenesis
- Gene Silencing
- Cell Death
- Dna Hypermethylation
- Cell Proliferation
- Demethylation
- Cell Growth
PTMs
- Methylation
- Demethylation
- Phosphorylation
- Reduction
- Cleavage
- Acetylation
Research Areas
- Apoptosis
- Cancer
- Chromatin Research
- Direct Reversal of DNA Damage
- DNA Repair
- Tumor Suppressors
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MGMT
bosterbio.com/bosterbio-gene-info-cards/MGMT
References
PMID: 2405387 by Tano K., et al. Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.
PMID: 2188979 by Rydberg B., et al. cDNA cloning and chromosomal assignment of the human O6- methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.
2 Publications of Boster MGMT Antibodies & ELISA Kits
- PubMed ID: 10.1007/s11670-008-0139-4, Expressions of MGMT and Survivin in colorectal carcinoma
- PubMed ID: 32881880, Minea RO,Duc TC,Swenson SD,Cho HY,Huang M,Hartman H,Hofman FM,Schönthal AH,Chen...
*More publications can be found for each product on its corresponding product page