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MGST1 / Microsomal glutathione S-transferase 1 · IHC design guide
Design Immunohistochemistry for MGST1
Plan chromogenic MGST1 IHC in paraffin sections using liver hepatocytes as a high-staining reference (HPA tissue IHC). This guide covers granular cytoplasmic scoring (HPA tissue IHC) and the 2 µg/mL catalog antibody condition (datasheet: PB9723).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
MGST1 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Granular cytoplasm in tissue sections (HPA tissue IHC) | |
| Staining pattern | Granular cytoplasmic staining in most tissues (HPA tissue IHC) | |
| Antigen retrieval | EDTA pH 8.0 HIER, heat-mediated (datasheet PB9723) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | Bone marrow+4 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep paraffin-section fixation consistent (standard IHC practice; not target-specific) | |
| Caveat | Staining and RNA show medium consistency (HPA tissue IHC) | |
| Regulation | High expression in liver (UniProt) | |
| Isoform / epitope | 2 isoforms; map epitopes to cytoplasmic or lumenal loops (UniProt) |
Section 1
Recommended MGST1 IHC & IF Protocols
The catalog antibody’s IHC-P protocol (datasheet PB9723) is paired with a published MGST1 protocol for paraffin-embedded aortic tissue (PMC9339483).
| Sample | Paraffin-embedded human pancreas cancer tissue; fixative not specified (datasheet PB9723) |
| Fixation | Image fixative and duration unreported (datasheet PB9723); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: EDTA pH 8.0 (datasheet PB9723); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% goat serum (datasheet PB9723) |
| Primary antibody | Rabbit anti-MGST1, 2-5μg/ml (datasheet PB9723) |
| Primary incubation | Overnight at 4 °C (datasheet PB9723) |
| Detection | HRP-conjugated secondary, DAB chromogen (datasheet PB9723) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | MGST1-positive staining in glandular cells of adrenal gland (HPA tissue IHC: High). HPA tissue profile: Cytoplasmic expression with a granular pattern in most tissues. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated EDTA pH 8.0 retrieval (datasheet PB9723); the published aortic protocol does not specify a retrieval condition (PMC9339483).
Section 2
What Is the Expected MGST1 Staining Pattern?
MGST1 should appear mainly as granular cytoplasmic staining in IHC sections (HPA: tissue IHC profile). Look for strong staining in liver hepatocytes and kidney collecting ducts, among other HPA-positive cells (HPA: High). Its membrane localization in the endoplasmic reticulum and mitochondrial outer membrane supports an extranuclear pattern (UniProt P10620 topology). HPA rates the tissue IHC profile Enhanced, while reporting medium consistency between staining and RNA data (HPA: tissue IHC reliability).
| Granular cytoplasmic staining in hepatocytes, with little nuclear staining (HPA: liver hepatocytes High; tissue IHC profile). | This fits the reported liver pattern and membrane localization (HPA: tissue IHC profile; UniProt P10620 subcellular location). Judge the signal in hepatocytes rather than by whole-section darkness: adjacent cell populations need not share the same staining level (HPA: cell-specific tissue IHC levels). |
| Predominantly nuclear staining, or a pattern with no discernible cytoplasmic component (HPA: expected tissue IHC profile). | Treat this as a compartment mismatch requiring investigation, since the reported IHC pattern is cytoplasmic and the annotated locations are membrane-bound (HPA: tissue IHC profile; UniProt P10620 subcellular location). Check morphology, counterstain and detection controls before calling it specific (general IHC practice). |
| Strong staining in cells reported as not detected, such as skeletal-muscle myocytes (HPA: skeletal muscle myocytes Not detected). | Unexpected signal may reflect cross-reactivity or endogenous detection activity (general IHC practice). Compare a no-primary control and an HPA-positive tissue processed in the same run; a single discordant section cannot establish new MGST1 expression (general IHC practice; HPA: tissue IHC levels). |
| Uniform brown haze across cells and extracellular spaces, obscuring granular detail (HPA: expected tissue IHC profile). | This is background rather than the reported cell-associated granular pattern (HPA: tissue IHC profile). Review blocking, washing and chromogen development, and inspect a no-primary control for detection-system background (general chromogenic IHC practice). |
| No detectable hepatocyte staining in an otherwise interpretable liver section (HPA: liver hepatocytes High). | A failed positive reference is inconclusive for MGST1 absence (general IHC practice; HPA: liver hepatocytes High). Verify tissue preservation and the staining run, then optimize retrieval or antibody conditions according to the antibody's IHC-P instructions (general IHC practice). |
💡Expected MGST1 appearanceCall a positive result when hepatocytes or another HPA High cell population shows distinct granular cytoplasmic staining, strongest in the expected cells; isolated nuclear signal or uniform haze is suspect (HPA: tissue IHC profile and cell levels; UniProt P10620 subcellular location; general IHC practice).
| Compartment and topology (UniProt P10620 topology). | MGST1 has four transmembrane segments and is assigned to ER and mitochondrial outer membranes (UniProt P10620 topology; subcellular location). Paraffin-section IHC reports their combined granular cytoplasmic appearance; it does not by itself resolve which organelle contributes each granule (HPA: tissue IHC profile; general IHC interpretation). |
| Choice of positive and low-signal tissue (HPA: tissue IHC levels). | Hepatocytes and kidney collecting ducts are High; adipocytes are Medium, while skeletal-muscle myocytes are Not detected (HPA: tissue IHC levels). Use a High population to assess whether the run worked. A weak result in a lower-signal population is a less decisive technical check (general IHC practice). |
| Evidence and antibody scope (HPA: tissue IHC reliability; antibody validation). | HPA labels the tissue IHC profile Enhanced but describes only medium consistency with RNA expression data (HPA: tissue IHC reliability). The listed antibody HPA044840 has Enhanced IHC validation (HPA: antibody validation). These labels support a reference pattern; they do not validate every antibody or explain each discordant sample (general interpretation). |
| Epitope and isoform uncertainty (UniProt P10620 topology; isoforms). | Two isoforms are listed, but the supplied record does not map the IHC antibody epitope or establish isoform-specific staining (UniProt P10620 isoforms; HPA: antibody record). Topology alone cannot predict retrieval requirements or fixation sensitivity (UniProt P10620 topology). Treat changes in those conditions as general IHC optimization, not a documented MGST1 effect. |
| IF/ICC Q&A: What localization should an IF image show? (HPA: subcellular ICC-IF). | HPA reports mainly mitochondrial signal with additional ER localization in ICC-IF (HPA: approved subcellular locations). That supports the organelle interpretation of cytoplasmic IHC staining, but IHC sections need not resolve mitochondria from ER (HPA: tissue IHC profile; general microscopy interpretation). IF/ICC conditions belong in its separate guide. |
| Situation | Likely cause | Next action |
|---|---|---|
| Liver positive reference is blank or very faint (HPA: liver hepatocytes High). | The run may have insufficient antigen exposure or detection sensitivity; a blank positive reference cannot distinguish technical failure from biology (general IHC practice). | Confirm hepatocyte morphology and reagent performance, then optimize retrieval and antibody conditions within the antibody's IHC-P workflow (general IHC practice). |
| Nuclear staining dominates the image (HPA: cytoplasmic tissue IHC profile). | The observed compartment conflicts with the annotated ER and mitochondrial outer-membrane locations (UniProt P10620 subcellular location). Nonspecific staining or interpretation of counterstain is possible (general IHC practice). | Review the counterstain and no-primary control; require a distinct cell-associated cytoplasmic pattern in a positive reference before scoring MGST1 (HPA: tissue IHC profile; general IHC practice). |
| A reported Not detected cell population stains strongly (HPA: tissue IHC levels). | Cross-reactivity or endogenous detection activity could explain unexpected signal (general IHC practice); HPA's cell-level reference alone cannot identify the mechanism (HPA: tissue IHC levels). | Compare with a no-primary control and a High reference on the same run; assess the suspect cell type separately from neighboring positive cells (general IHC practice; HPA: cell-specific tissue IHC levels). |
| Diffuse brown haze masks cell boundaries (HPA: granular cytoplasmic tissue IHC profile). | Background from detection reagents, incomplete blocking or excessive development is possible (general chromogenic IHC practice). | Inspect a no-primary control, review blocking and wash steps, and shorten development if controls show excess background (general chromogenic IHC practice). |
| Different tissue regions appear to disagree (HPA: cell-specific tissue IHC levels). | The sampled regions may contain different cell populations with different reported MGST1 levels (HPA: tissue IHC levels); section quality or uneven staining is also possible (general IHC practice). | Score identifiable cells with their morphology and counterstain, and compare equivalent regions on a positive reference before changing the interpretation (general IHC practice). |
| IHC looks granular, but its pattern does not identify a single organelle (HPA: tissue IHC profile). | MGST1 is assigned to both ER and mitochondrial outer membranes (UniProt P10620 subcellular location), while HPA's IHC description is cytoplasmic and granular (HPA: tissue IHC profile). | Report the IHC result as granular cytoplasmic staining. Use the separate IF/ICC localization evidence when an organelle-level comparison is needed (HPA: approved subcellular ICC-IF locations; general IHC interpretation). |
Experiment evidence
IHC & IF images showing MGST1 staining
PB9723 · IHCIHC analysis of MGST1 using anti-MGST1 antibody (PB9723). MGST1 was detected in a paraffin-embedded section of human pancreas cancer tissue.
PB9723 · IHCIHC analysis of MGST1 using anti-MGST1 antibody (PB9723). MGST1 was detected in a paraffin-embedded section of human stomach cancer tissue.
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000008394-MGST1/tissue/cerebral+cortex#imid_12330325
HPA · StomachHuman Protein Atlas IHC, Stomach. Source: https://www.proteinatlas.org/ENSG00000008394-MGST1/tissue/stomach+2#imid_12317753
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000008394-MGST1/tissue/colon#imid_12317795
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000008394-MGST1/tissue/liver#imid_12317788
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA044840; green = MGST1, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA044840; green = MGST1, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for MGST1 IHC & IF
🧪Run liver first: hepatocytes should stain strongly (HPA: High in liver hepatocytes). Use skeletal muscle myocytes as the negative tissue (HPA: Not detected in skeletal muscle myocytes); on the liver slide, count morphologically distinct non-hepatocyte cells as internal negatives only if they show no DAB signal above background, because their MGST1 status is not specified in the supplied HPA rows.
Positive control tissue: Adrenal gland (Glandular cells, HPA High)
Negative control tissue: Bone marrow (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show MGST1 in A-431, U-251MG, U2OS, with annotated localisation: Mitochondria (approved) (HPA subcellular).
Technical controls: Include a no-primary (secondary-only) section, a host- and clonality-matched rabbit IgG isotype control, and MGST1-knockout material or a validated peptide-block control (caption: rabbit anti-MGST1 primary; standard IHC practice). Block endogenous peroxidase before developing the liver section with HRP/DAB (caption: HRP/DAB detection; standard IHC practice).
⚠️Feasibility: The selected PB9723 paraffin-section caption reports heat-mediated retrieval in EDTA at pH 8.0, but its fixative is unreported; no target-specific fixation window or fixation effect is supplied (PB9723 tissue-IHC caption). That caption supports a retrieval-based paraffin IHC starting point, but the supplied evidence does not establish whether frozen sections or IF are easier (PB9723 tissue-IHC caption; HPA: approved mitochondrial and additional ER ICC-IF localization). Watch for endogenous peroxidase background in liver when interpreting HRP/DAB staining (standard IHC practice).
HPA tissue IHC evidence for MGST1
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Enhanced — Medium consistency between antibody staining and RNA expression data.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Appendix | Enterocytes | High | Protein (IHC) | HPA → |
| Endometrium | Ciliated epithelial cells | High | Protein (IHC) | HPA → |
| Kidney | Collecting ducts | High | Protein (IHC) | HPA → |
| Liver | Hepatocytes | High | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Epididymis | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Oral mucosa | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | Myocytes | Not detected | Protein (IHC) | HPA → |
| Smooth muscle | Smooth muscle cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced MGST1 IHC Tips
Use the catalog antibody’s paraffin-section IHC conditions as the starting point, then check whether staining matches MGST1’s expected cells and compartments.
Which antigen retrieval conditions should I try first for MGST1 IHC?
Start with heat-mediated retrieval in EDTA, pH 8.0, for paraffin sections (datasheet PB9723). The catalog antibody’s tissue-IHC image used that retrieval, followed by 10% goat-serum blocking and 2 μg/ml primary antibody overnight at 4°C (datasheet PB9723). If staining is weak, adjust heating and cooling while keeping the buffer constant, and compare treated sections in the same run (standard IHC practice). Judge improvement by clearer granular cytoplasmic staining in an expected positive population, such as hepatocytes, rather than stronger staining everywhere (HPA: granular cytoplasmic pattern; High in hepatocytes). The caption does not state the tissue fixative, so it cannot establish retrieval performance across fixation conditions (datasheet PB9723).
How should I troubleshoot fixation-related loss of MGST1 staining?
MGST1-specific sensitivity to fixation is unknown from the supplied evidence; the catalog antibody’s paraffin-section caption does not report a fixative (datasheet PB9723). Record the fixative and fixation duration for each specimen, then compare sections processed and stained together (standard IHC practice). Keep retrieval at EDTA, pH 8.0, and primary antibody at 2 μg/ml overnight at 4°C while evaluating a processing difference (datasheet PB9723). Use an expected positive cell population, such as hepatocytes, to distinguish a weak run from a low-expressing specimen (HPA: High in hepatocytes). Neither the tissue-IHC pattern nor MGST1’s membrane topology establishes how fixation affects this epitope (HPA: tissue IHC; UniProt P10620 topology).
Where should convincing MGST1 staining appear in chromogenic sections?
Look for a granular cytoplasmic pattern rather than isolated nuclear staining (HPA: granular cytoplasmic expression in most tissues). MGST1 resides in the endoplasmic reticulum membrane and mitochondrial outer membrane (UniProt P10620 subcellular location); mitochondrial localization is the main approved location in cell imaging, with additional endoplasmic reticulum localization (HPA: subcellular). At light-microscope resolution, score staining within the correct cells and cytoplasmic compartment without assigning individual DAB granules to one organelle (standard IHC practice). Hepatocytes and kidney collecting ducts offer expected positive populations, while skeletal myocytes are listed as not detected (HPA: High in hepatocytes and collecting ducts; Not detected in skeletal myocytes). Check these patterns against a negative reagent control before interpreting diffuse color as MGST1 (standard IHC practice).
Can this IHC antibody distinguish MGST1 isoforms or epitope accessibility?
The record lists 2 MGST1 isoforms, but the supplied IHC caption does not map the catalog antibody’s epitope or establish isoform specificity (UniProt P10620 isoforms; datasheet PB9723). MGST1 has 4 annotated transmembrane segments and short lumenal regions, so epitope position could affect accessibility after processing (UniProt P10620 topology). Acetylated lysines are reported at residues 42, 55, and 60; without an epitope map, their effect on this antibody remains unknown (UniProt P10620 modified residues; datasheet PB9723). Use the documented EDTA, pH 8.0 retrieval as the comparison baseline (datasheet PB9723). If staining changes with processing, describe the observed staining difference rather than assigning it to an isoform or modification (standard IHC practice).
How can IF help assess an ambiguous MGST1 IHC pattern?
Use IF as a separate localization check: MGST1 is mainly mitochondrial in approved cell imaging, with additional endoplasmic reticulum localization (HPA: subcellular). Multiplex with a marker identifying the expected cell population, such as hepatocytes when examining liver, and assess signal within those cells (HPA: High in hepatocytes; standard IF practice). Select fluorophores after checking tissue autofluorescence, and include single-channel and secondary-only controls to identify bleed-through or background (standard IF practice). Choose permeabilisation for the antibody epitope’s side of the membrane; MGST1 has both cytoplasmic and lumenal regions, while this antibody’s epitope is unspecified (UniProt P10620 topology; datasheet PB9723). Do not transfer the paraffin-section EDTA, pH 8.0 retrieval condition directly to IF (datasheet PB9723; standard IF practice).
What should I check when MGST1 DAB staining is widespread or diffuse?
First compare the section with a no-primary control and inspect whether color follows tissue edges, damaged areas, or every cell equally (standard IHC practice). Block endogenous peroxidase before HRP detection, and assess nonspecific binding with an appropriate protein block (standard IHC practice). The catalog image used 10% goat serum, an HRP-linked secondary incubated for 30 minutes at 37°C, and DAB development (datasheet PB9723). Recheck the 2 μg/ml primary concentration and overnight 4°C incubation before increasing detection intensity (datasheet PB9723). True tissue staining should be judged against MGST1’s reported granular cytoplasmic pattern and cell-specific expression, rather than DAB intensity alone (HPA: tissue IHC).
How should I quantify MGST1 staining across paraffin sections? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and tissue area before scoring, and apply the same threshold and imaging settings across sections (standard IHC practice). For cell-level analysis, report the percentage of positive cells with an H-score based on staining intensity; for spatial analysis, report positive-cell density per mm² (standard IHC practice). Normalize counts to eligible nucleated cells or measured viable tissue area, excluding folds, edges, and necrosis using prespecified rules (standard IHC practice). Score cytoplasmic staining in the relevant population rather than pooling unlike compartments or cell types (HPA: granular cytoplasmic expression; standard IHC practice). Record retrieval at EDTA, pH 8.0 and primary concentration at 2 μg/ml when comparing runs (datasheet PB9723).
How can I distinguish true MGST1 positivity from staining artefacts?
Give greatest weight to granular cytoplasmic staining in an expected cell population, such as hepatocytes or kidney collecting ducts (HPA: granular cytoplasmic pattern; High in hepatocytes and collecting ducts). Treat nuclear-only staining or uniform color across cell types as suspect because MGST1 is assigned to endoplasmic reticulum and mitochondrial membranes (UniProt P10620 subcellular location; standard IHC practice). Compare tissue edges and necrotic regions with intact interior tissue, and check a no-primary control for nonspecific DAB signal (standard IHC practice). Confirm that endogenous peroxidase was blocked before interpreting HRP/DAB deposits (standard IHC practice). HPA rates tissue-IHC reliability as Enhanced but reports only medium consistency with RNA, so an isolated positive field warrants cautious interpretation (HPA: reliability description).
Boster reagents
Best MGST1 / Microsomal glutathione S-transferase 1 IHC Antibodies
PB9723 has IHC images from human pancreas cancer, stomach cancer, and liver paraffin sections (PB9723 image captions). M04703 lists human, mouse, and rat reactivity for IHC and IF/ICC, without image evidence (M04703 catalog).
Real IHC data
Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody ®
Cat # PB9723
PB9723 is the only card that will render; its IHC images show human pancreas cancer, stomach cancer, and liver paraffin sections (PB9723 image captions). Its catalog lists human, mouse, and rat reactivity, but reports IHC-specific testing in human samples (PB9723 catalog).
Which to pick: Choose PB9723 for human paraffin-section IHC: its captions document EDTA pH 8.0 retrieval and 2 μg/ml primary antibody; the fixative is unreported (PB9723 image captions). For IF/ICC, M04703 lists those applications and is a rabbit monoclonal, but has no IF image in the payload (M04703 catalog). For mouse or rat work, both SKUs list reactivity with those species; confirm IHC performance in your samples because PB9723’s IHC-specific testing and images are human, while M04703 has no IHC images here (PB9723 catalog and image captions; M04703 catalog).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry P10620 (MGST1_HUMAN, Microsomal glutathione S-transferase 1).
- Human Protein Atlas. MGST1 tissue IHC expression (reliability: Enhanced).
- Human Protein Atlas. MGST1 subcellular location (ICC-IF): Mainly localized to the mitochondria. In addition localized to the endoplasmic reticulum..
- Human Protein Atlas. MGST1 antibody validation summary (1 antibodies).
- MGST1 facilitates novel KRAS(G12D) inhibitor resistance in KRAS(G12D)-mutated pancreatic ductal adenocarcinoma by inhibiting ferroptosis. Molecular medicine (Cambridge, Mass.) 2024 — PMC11536589.
- Microsomal glutathione transferase 1 controls metastasis and therapeutic response in melanoma. Pharmacological research 2023 — PMC10623471.
- Aging Alters the Aortic Proteome in Health and Thoracic Aortic Aneurysm. Arteriosclerosis, thrombosis, and vascular biology 2022 — PMC9339483.
- Single-Cell RNA-Seq Analysis of Cells from Degenerating and Non-Degenerating Intervertebral Discs from the Same Individual Reveals New Biomarkers for Intervertebral Disc Degeneration. International journal of molecular sciences 2022 — PMC8999935.
- PubMed PMID:3372534 — UniProt-cited evidence.
- PubMed PMID:8812420 — UniProt-cited evidence.
- PubMed PMID:10524215 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for MGST1 (Microsomal glutathione S-transferase 1). Expected localisation Granular cytoplasm in tissue sections (HPA tissue IHC). Antigen retrieval: EDTA pH 8.0 HIER, heat-mediated (datasheet PB9723). Validated IHC antibody: PB9723 at 2-5μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative Bone marrow. IHC-P workflow on paraffin sections; fixative unspecified. Protocol steps: 1. Sample: Paraffin-embedded human pancreas cancer tissue; fixative not specified (datasheet PB9723); 2. Fixation: Image fixative and duration unreported (datasheet PB9723); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: EDTA pH 8.0 (datasheet PB9723); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% goat serum (datasheet PB9723); 8. Primary antibody: Rabbit anti-MGST1, 2-5μg/ml (datasheet PB9723); 9. Primary incubation: Overnight at 4 °C (datasheet PB9723). Watch for: Fixation: Keep paraffin-section fixation consistent (standard IHC practice; not target-specific); Caveat: Staining and RNA show medium consistency (HPA tissue IHC); Isoform / epitope: 2 isoforms; map epitopes to cytoplasmic or lumenal loops (UniProt); Regulation: High expression in liver (UniProt). Evidence: UniProt P10620, HPA tissue IHC · Enhanced, HPA ICC-IF · Mainly localized to the mitochondria. In addition localized to the…, Datasheet PB9723, PMC9339483. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/MGST1/immunohistochemistry-ihc-guide.