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We validate the specificity of these antibodies to Helicase MOV-10 by testing them on tissues known to express MOV10 positively and negatively. Browse below to find the MOV10 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.
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Facts about Helicase MOV-10.
In collaboration with FMR1, regulates miRNA- mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE- 1) in collaboration with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941).
|DNA2/NAM7 helicase family|
DKFZp667O1423; EC 3.6.1; EC 18.104.22.168; FLJ32791; KIAA1631mouse) homolog; Mov10, Moloney leukemia virus 10, homolog (mouse)
|Sequence:||1; NC_000001.11 (112674312..112702377)|
Cytoplasm, P-body. Cytoplasm, Cytoplasmic ribonucleoprotein granule. Cytoplasm, Stress granule. Nucleus. Cytoplasm. Co-enriched in cytoplasmic foci with TUT4 (PubMed:30122351). In developing neurons, localizes both in nucleus and cytoplasm, but in the adulthood it is only cytoplasmic (By similarity). After infection, relocalizes to the DENV replication complex in perinuclear regions (PubMed:27974568).
This article will cover the validation and application of Boster Bio's MOV10L1 antibody picoBand. We'll discuss the benefits of the MOV10 mark, its use in research and how it can aid you understand RNA-helicases. Whether you're looking for a high affinity antibody for RNA helicases, or you're looking for a specific molecular target, this product has a lot to offer.
Scientists around the world can submit their results to receive product credits for Boster Bio's Anti-RNA Helisome Mov10l1MOV10LAntibody PikoBand. The product can be stored at either -20 C for 1 year or at 4 C until it is used. To prolong the product's shelf life, it should not be repeated freezing or thawing.
The MOV10 gene is a transcription determinant that associates with synapses and mRNAs. It controls the local translation target mRNAs. It is not yet clear how MOV10 works in synapses. In fact, the gene has not been fully studied. This article will discuss the mechanism and use of the MOV10 gene marker. We will also talk about its functional roles in brain.
The MOV10 genes has antiviral qualities. When it is expressed in cells, it protects them from the Influenza A virus. However, MOV10 can cause cells to become more susceptible to the disease if it is mutated. The antiviral properties of MOV10 against IAV may result from its ability to disrupt NP’s nuclear distribution. MOV10 is also able to block the function RdRp.
The MOV10 protein encodes a protein that protects the mRNAs against AGO binding. The gene is related to Fragile X a protein that causes mental retardation. Its C-terminus binds a variety proteins in the cell, including AGO (a core RISC component). MOV10 is required to interact with AGO. It also helps suppress a reporter gene via miR-21.
Moreover, it has been associated with the mRNA governing the development of neurons. Its knockout mutations in neurons increase neurite growth. Therefore, MOV10 has many functions and its regulation is still in its infancy. Although its regulation is only starting to be understood, its important role in the brain is clear. The research is still ongoing. It's likely that many of us will be surprised to learn about the MOV10 gene.
In vitro the ORF1-ORF2 gene products can form cytoplasmic foci. LINE-1 foci may be associated with stress granules. These granules contain translation-dormant mRNA. These foci can also undergo degradation. These foci can also be degraded. MOV10 was shown to associate with LINE-1 RNA, cytoplasmic LINE-1RNPs, and LINE-1 RNA.
A gene called MOV10 inhibits LINE-1 retrotransposition in HeLa cells. A plasmid DNA, pcDNA3.1, containing the neomycin-resistant gene, was transfected into cells. Cell colonies were then selected. The results of PCR were analyzed using crystal violet staining and were summarized in the form of a bar graph. Arbitrarily, 100 cell colonies were left without MOV10.
The MOV10 gene first was predicted to be a helicase. Eventually, mov10 became a helicase. Its helicase motifs were located near its Cterminus, between residues 524 to 911. Additional domains are also provided by both the C-termini and N. These domains are important for nuclease activity and NA binding.
The detection of specific noncoding RNAs has allowed validation of the MOV10 gene marker. The gene encoding MOV10 is known as gb110 and is associated with PBs and SGs, which are membraneless structures enriched in mRNA decay machinery. This marker also associates with several proteins involved SGs including the mRNA decapping enzyme subunit
As an antiviral protein, MOV10 has been shown to inhibit the activity of various viruses, including LINE-1. This mechanism helps cells protect themselves from intracellular injury and to fight infectious agents. MOV10 inhibits LINE-1 virus, but is also effective against other retroviruses. MOV10, for example, is an antiviral antibody.
We used antibodies against GAPDH (MOV10) and MOV10 to validate the MOV10 markers. Then, we performed immunoblotting using purified chromatin fractions and nuclear fractions and compared these results with control samples. We also determined endogenous levels of MOV10 in primary human fibroblasts. The results showed that MOV10 is associated with chromatin, and the association between MOV10 and chromosomes is critical for local translation of proteins.
MOV10 plays a key role in brain development by controlling RNA helicase activity, and the expression of DNA. It is also involved mRNA production, cell metabolism, and antiviral action. It has also been implicated as a tumorigenic agent. Future directions for MOV10 are the development of conditional knockout mouse models, studies using MOV10 tumor markers, and understanding MOV10's role in learning, memory, and learning.
The MOV10 protein was initially predicted to be an RNase helicase. Molecular analysis has confirmed that the MOV10 protein, which unwinds 5'-overhang duplex RNAs, is an ATP-dependent helicase. Seven highly conserved helicase motif motifs are found in the C-terminal region MOV10. Helicases include proteins with ATP-dependent structure remodelling activity and classical double stranded nucleic Acid unwinding activities. The seven characteristic motifs can be found in the superfamilies I and II, which are the two largest helicase genus groups.
Although the MOV10 gene can be expressed in all organisms, most research has focused on its function within the brain. Knockdown MOV10 in mice causes gastrulation to be slowed and can disrupt spermatogonial precursor cell proliferation. MOV10 also interacts with neuron projection proteins and cytoskeleton transcript mRNAs. Mutant mice have reduced hyperactivity, dendritic arborization (DAR), and other behavioral defects.
The validation of MOV10 gene has shown that this marker is an excellent candidate for antiviral studies. This gene is associated with argonaute and regulates microRNA metabolism. It prevents LINE-1 mobilisation. MOV10 plays a crucial role in the host's defense from parasitic genetic elements. Its role is not fully understood in the immune system.