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- Table of Contents
MYO1C / Unconventional myosin-Ic · Western blot design guide
Design a Western Blot for MYO1C
Source-linked MYO1C Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-MYO1C WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
MYO1C Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~121.7 kDa | |
| Gel | 8–10% (standard starting point) | |
| Positive control | Appendix (IHC candidate; verify WB) +4 more | |
| Negative control | Bone marrow (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Phosphorylated + Acetylated | |
| Caveat | Phosphorylation-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 3 isoform(s) |
Section 1
Source-Linked MYO1C Western Blot Protocol Options
The A03399 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | extracts of various cell lines, (catalog A03399) |
| Gel % | 8–10% (standard starting point) |
| Load | 20–30 µg total protein per lane; optimize for abundance (standard starting point) |
| Transfer | Wet/tank transfer; optimize duration (standard starting point) |
| Membrane | 0.45 µm PVDF (standard starting point) |
| Blocking | 5% milk or 5% BSA in TBST (standard starting point) |
| Primary antibody | A03399; use the WB datasheet starting dilution (standard starting point) |
| Primary incubation | Overnight at 4 °C (standard starting point) |
| Secondary antibody | Species-matched HRP conjugate at validated dilution (standard starting point) |
| Secondary incubation | 1 h at room temperature (standard starting point) |
| Wash | 3 × 5 min in TBST (standard starting point) |
| Detection | ECL; bracket exposures to avoid saturation (standard starting point) |
Section 2
What Is the Expected MYO1C Western Blot Band Size?
MYO1C has a predicted mass of 121.7 kDa; three isoforms could affect migration, but no distinct band sizes are demonstrated.
| Band near 121.7 kDa | consistent with the predicted MYO1C mass; confirm identity with an antibody control |
| Several bands near the expected region | could reflect isoforms 1, 2, and 3 if their migration differs |
| Single band despite three isoforms | the isoforms may not resolve as separate bands |
| Weak band in a soluble fraction | MYO1C may be retained in membrane or cell cortex fractions |
💡Expected MYO1C appearanceUniProt predicts 121.7 kDa for MYO1C; no empirical band size is supplied, and isoform-dependent migration requires band-identity controls.
| UniProt predicted mass | places the reference band near 121.7 kDa |
| Splice isoform 1 | its individual mass and migration relative to the other isoforms are not supplied |
| Splice isoform 2 | may differ in size from the other isoforms, but its mass is not supplied |
| Splice isoform 3 | may differ in size from the other isoforms, but its mass is not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | MYO1C extraction may be incomplete from membrane or cell cortex fractions | check the insoluble fraction and a positive control lysate |
| Band higher than expected | band identity or migration is uncertain | compare with a positive control and verify antibody specificity |
| Band lower than expected | an isoform or unrelated band is possible; individual isoform masses are unavailable | check antibody specificity and compare isoform expression |
| Multiple bands | isoforms 1, 2, and 3 may contribute if they migrate differently | verify bands with isoform-aware controls |
| Weak or no signal | MYO1C abundance or extraction may be low in the sampled fraction | check loading and examine membrane-associated fractions |
Experiment evidence
Western blot images showing MYO1C band
A03399 · MYO1C
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MYO1C Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for MYO1C in Western blot, you can use appendix tissue, which HPA rates High.
Positive control: Appendix (IHC candidate; verify WB)
Negative control: Bone marrow (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: MYO1C is intracellular, and HPA reports bone marrow as not detected, making tissue lysates feasible controls.
HPA tissue expression evidence for MYO1C
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Appendix | glandular cells | High | Protein (IHC) | HPA → |
| Colon | glandular cells | High | Protein (IHC) | HPA → |
| Duodenum | glandular cells | High | Protein (IHC) | HPA → |
| Gallbladder | glandular cells | High | Protein (IHC) | HPA → |
| Lung | endothelial cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Skeletal muscle | myocytes | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Low | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Low | Protein (IHC) | HPA → |
| Spleen | cells in red pulp | Low | Protein (IHC) | HPA → |
Section 3
Advanced MYO1C Western Blot Tips
Deeper troubleshooting and optimisation questions for MYO1C, answered from its protein features.
How should MYO1C band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could MYO1C isoforms produce different bands?
Isoforms · Three isoforms are listed. Relative to the canonical sequence, isoform 2 lacks residues 1–35, while isoform 3 replaces residues 1–25 with MRYRAS. Check whether the antibody recognizes the altered N terminus before comparing bands; the features do not establish their apparent sizes.
Which MYO1C modifications matter when interpreting bands?
PTM · UniProt lists N6-methyllysine 383, phosphoserine 408, N6-acetyllysine 486, phosphoserine 536, phosphoserine 864, and the supplied annotation ‘Phosphoserine N-acetylmethionine’ at 1041. These are UniProt coordinates; antibody or paper numbering may differ. Their presence alone does not establish a visible band shift.
Does this guide establish induction of MYO1C?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for MYO1C Western blot?
Transfer · MYO1C is predicted at 121.7 kDa. Check transfer efficiency near that mass when setting up the blot. The supplied features do not specify a transfer method or membrane.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A03399 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should MYO1C bands be quantified across samples?
Quantitation · Use the same band assignment and sample fraction across comparisons. MYO1C is listed in the cytoplasm and nucleus, with additional cortex, membrane, and vesicle locations; a change in one fraction need not represent a change in total MYO1C.
Should MYO1C run at its predicted 121.7 kDa?
Interpretation · Use 121.7 kDa as the calculated reference. No observed band size is supplied, and the listed features do not establish an apparent mass or explain a shift.
Compare each band with the 121.7 kDa predicted mass and the three listed isoforms. Check antibody recognition of their differing N termini and compare fractions, given MYO1C’s multiple listed locations. The supplied features alone cannot identify an unexpected band.
Neither a signal peptide nor a propeptide is listed. Do not assign a smaller band to cleavage of either based on these features.
Compare each band with the 121.7 kDa predicted mass and the three listed isoforms. Check antibody recognition of their differing N termini and compare fractions, given MYO1C’s multiple listed locations. The supplied features alone cannot identify an unexpected band.
Neither a signal peptide nor a propeptide is listed. Do not assign a smaller band to cleavage of either based on these features.
Boster reagents
MYO1C Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Unconventional myosin-Ic MYO1C Antibody
Cat # A03399
The catalog reports anti-MYO1C antibody A03399 for Western blot, with reported Human, Mouse, and Rat reactivity. A WB image is available for extracts from various cell lines; the supplied caption does not identify the lines or establish validation across all listed species.
Which to pick: A03399 is the only listed MYO1C antibody and has a WB image. Check that its reported Human, Mouse, or Rat reactivity fits your sample; the supplied caption does not name the cell lines tested.
Source: BosterBio MYO1C gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry O00159.
- Human Protein Atlas. MYO1C tissue expression.
- PMC8229726 — target-verified WB comparison
- PMC5600340 — target-verified WB comparison