Nuclear factor 1 C-type Antibodies

Nuclear factor 1 C-type (NFIC)

Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

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Gene Information

Human
Gene Name:	NFIC
Uniprot:	P08651
Entrez:	4782

Family

Belongs to:
CTF/NF-I family

Alternative Names

CCAAT-binding transcription factor; CTF5; CTFCCAAT-box-binding transcription factor; MGC20153; NF1-C; NFI; NF-I; NF-I/C; NFI-C; nuclear factor 1 C-type; Nuclear factor 1/C; nuclear factor I/C (CCAAT-binding transcription factor); Nuclear factor I/C; TGGCA-binding protein

Molecular Weight

Mass (kDA):
55.675 kDA

Genomic Context

Human
Location:	19p13.3
Sequence:	19; NC_000019.10 (3359630..3469217)

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Mammary Neoplasms
• Neurofibromatosis 1
• Liver Carcinoma
• Carcinoma
• Congenital Absence
• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Breast
• Malignant Neoplasms
• Malignant Paraganglionic Neoplasm
• Hydrocephalus
• Nervousness
• Infective Disorder

• Neoplasm Metastasis
• Coronary Heart Disease
• Asymptomatic Infections
• Abscess
• Metastatic Malignant Neoplasm To The Lymph Node
• Melanoma
• Glioma

Pathways

• Dna Replication
• Root Development
• Odontoblast Differentiation
• Cell Migration
• Cell Proliferation
• Rna Splicing
• Cell Adhesion
• Mammary Gland Involution
• Developmental Process
• Osteoblast Differentiation
• Hypersensitivity
• Regulation Of Gene Expression
• Ossification

• Mesenchymal Cell Proliferation
• Viral Transcription
• Translation
• Axon Guidance
• Cell Cycle
• Cell Differentiation
• Liver Regeneration

PTMs

• Reduction
• Phosphorylation

• Glycosylation
• Cleavage

• Oxidation

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NFIC

Best Uses Of The NFIC Marker

The NFIC marker can be used for several purposes. Whether your goal is to identify a gene, identify a polymorphism in a DNA strand, or find out the RNA sequence of a cell, NFICs can help you do so. These markers can be encapsulated in an EV or a SCAP. Multichannel pipettes may be more appropriate for specific uses. For large quantities of samples, a multichannel pipette is best.

NC-EV

The NFIC marker is a therapeutic tool with many potential applications. Its role has been well documented in odontoblast division. Using lentivirus transcription, NFIC was significantly overexpressed among SCAPs. Quantitative analysis showed that SCAPs with NFIC overexpression had higher levels of DMP-1, DSPP. This gene plays a role in the formation and maintenance of dentin.

NFIC protein levels were significantly higher in OE-NFIC-SCAPs when compared with the NC-NFIC-SCAPs after LPS10-EV treatment. Western blot analysis revealed that the EVs produced significantly more NFIC proteins than the control group. Boster has many high affinity antibodies in its arsenal. The NFIC antibody has been validated in Western blot, immunohistochemistry, and ELISA.

SCAPs

The NFIC marker's SCAP contains an encapsulated prot that can be used therapeutically. Its bioactivity is dependent on its ability to replenish NFIC within the SCAP. The researchers found that the protein can promote dentin growth. The researchers also reported that NFIC EV can promote odontoblastic differentiate, dentin production, and migration SCAPs. These findings suggest that NFIC is a potent therapeutic target for AP, skeletal developmental diseases, and other dental problems.

LPS activates the NFIC marker SCAPs and induces the release of several inflammatory mediators. LPS and IL-8 effects on NFIC expression were assessed by Western blotting, real-time polymerase Chain reaction, and Western blotting. NFIC silencing could also be determined by small interfering RNA. Enzyme-linked immunoassay was used to determine protein levels.

SCAPs were determined using the Cell Cycle Detection Kit by KeyGen BioTech. The cells were seeded on 96-well plates, with three 103 cells each well. Control groups were cells transduced by pLentiCherry, and normal untreated cell populations. The cells were then cultured in 2% FBS with 20 mL of MTT, 4,5-dimethylthiazolium bromide. Each culture was then stained with a color to see the MTT results.

In vitro NFIC was shown in SCAPs to increase mineralization and ALP activities. During this process, SCAPs were transduced with NFIC or with an empty vector. The cells were cultured in osteo/odontogenic medium for four to seven, fourteen, and 21 days. After these times, the cells developed mineralized nodules. The nodules were then stained with Alizarin Red and the absorbance was measured at 520 nm.

Gene infographics

Boster Bio has created gene infographics that provide a visual overview for each gene in humans as well as mice. These graphics include basic information about each gene, and the search bar makes it easy to find the gene of interest. The information in these infographics can help you understand the relationship between the gene and the body's functions. Boster Bio gene infographics are great for learning more about each individual gene, regardless what topic you are researching.

Steven Boster

Dr. Steven Boster used NFIC markers as one of the most efficient tools to identify patients in his clinic. He was a huge supporter of the southern gospel music genre and would sing so low that it was difficult to hear. He was also a huge enthusiast of auto racing and would often attend races on weekends, or additional events. An NFIC marker is a great way for Steven Boster to be remembered.