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- Table of Contents
Facts about Neurogenic locus notch homolog protein 3.
Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). .
Human | |
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Gene Name: | NOTCH3 |
Uniprot: | Q9UM47 |
Entrez: | 4854 |
Belongs to: |
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NOTCH family |
CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3
Mass (kDA):
243.631 kDA
Human | |
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Location: | 19p13.12 |
Sequence: | 19; NC_000019.10 (15159038..15200995, complement) |
Ubiquitously expressed in fetal and adult tissues.
Cell membrane; Single-pass type I membrane protein.; [Notch 3 intracellular domain]: Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
Notch is a crucial gene for many applications, including in cancer research. It is however often overlooked in basic science. Notch is involved many processes, such as patterning and metastasis. Boster scientists can submit their results to gain product credits. This technology is available for scientists worldwide. Find out the best uses of the NOTCH3 marking. These topics will be explored further.
The Notch3 antibody is able to identify cancer cells in many different ways. The antibody has anti-tumor properties and inhibits growth of basal cells lines. An agonist can enhance the transformed appearance. This gene signature is associated with several components of Notch, including the oncogene C-Myc and Id4, as well as the mammary stem cells regulator Id4. It is surprising that the ligand independent induction mechanism is linked to cancer progression and oncogenesis. The mechanism of cancer progression and oncogenesis are the best uses for the NOTCH3 marker in breast carcinoma research.
Notch pathway activity has a significant role in tissue self organization and notochordmorphogenesis. This pathway is being studied in detail, including the role of Notch receptors and its ligands. Understanding the development of tissues in the body is possible only through Notch signalling. Stem cell research often relies on notch pathway-positive cells, as they are essential to the development or the notochord.
A novel antibody targeting Notch3 has been developed to treat CADASIL, a neurological disorder caused by mutations in NOTCH3 gene. Notch3 antibody therapy reverses the characteristics of PAH in two rodent models without the toxic side effects of drugs, and it can also be used to screen for cancer cells. This marker is best used for research and development. It is crucial in finding the right treatment.
You can test the effects that siRNA has on Notch4 using a variety of methods. This can be demonstrated using lentivirus. A549 cells were transduced by lentivirus stocks, and kept in RPMI-1640 media containing puromycin for seven day. Quantitative real-time PCR was used to measure mRNA levels and western blotting was used to determine protein levels. Molecular Cloning was used in the creation of the flag-tagged Notch4 protein gene.
This biomarker is a novel one for the detection and monitoring of tumors. It was created by the recently discovered NOTCH3 gene. Notch3 activation requires cell to cell contact. However, the interaction does not need to be restricted only to cancer cells. Recent data show that patients with advanced TNBC may experience poor clinical outcomes if they have an over-expressed NOTCH3. These findings provide preclinical support for novel clinical trials that target the NOTCH3 gene in order to improve progression-free survival for TNBC patients.
Previously, researchers focused on downstream signalling of Notch receptors. Notch receptor ligands regulate Notch pathway activities, which control cell proliferation, migration and apoptosis. This biomarker also inhibits apoptosis in cells exposed to hypoxia. These new developments, however, have been accompanied in part by a lackof detailed knowledge regarding the biological role of Notch.
The new biomarker is highly expressed in platinum resistance cancer cells but not sensitive cancer cells. Using a western blot method, the authors found that notch2 and Notch2 proteins were expressed more abundantly in platinum-resistant than in platinum-responsive cancer cells. Additionally, Jagged1 protein levels and mRNA were higher than in OV2008 cell cells. The findings were confirmed by tissue samples which were platinum-resistant as well as those that were platinum responsive to the drugs.
Researchers in the field o cancer immunohistochemistry created antibodies to recognize NOTCH3 protein in tumor cells. Notch3 has been identified as a potential biomarker for the cancer drug BRAF. Antibody to NOTCH3 blocks the interaction between JAG-1 and NOTCH3 proteins, which is a key pathway for tumor cells' migration and invasion. It is possible reverse the PAH characteristics in two rodent models of mice by blocking the pathway between the two molecules. This new treatment might be able address the current shortage of disease-modifying therapies.
Hypoxia-induced LUAD cell growth and metastasis is mediated by the Notch4/ERK/JNK/P38 MAPK axis. This is a novel therapeutic target. The Notch4-ERK/JNK/P38 MAPK axis is involved in hypoxia-induced LUAD cell growth and metastasis. These findings are based in part on a recent study on LUAD cell growth. The Tongji Hospital approved the study.
Researchers have identified the key molecules that control the cell cycle of cancer cells. This may improve early detection of cancer by identifying their role in tumours. This research also helps researchers develop novel cancer therapies. The research team hopes the collaboration with the Institut Pasteur will improve cancer diagnostics. Although the results are preliminary, they suggest the NOTCH3 gene could play an important role in the development drugs for this disease.
The Notch homolog 1 translocation-associated (NOTCH) receptor plays an important role in gastric cancer cell proliferation and migration. This receptor is targeted to inhibit gastric cancer cell growth by inducing death. However, it has other benefits than tumor suppression. Notch signaling is also implicated with other types of cancer such as prostate and melanomas.
After ligand binding, NICD is released by the Notch receptor. It forms a complex in the CSL and promotes recruitment of co-activators. NICD then activates a large number of target genes in both the Hey and Hes families. By inhibiting this pathway, drugs targeting Notch-targeted cancer may be developed that are more specific to the disease.
Notch also plays an important role in synaptic plasticity (synaptic plasticity), spatial learning, memory, and dendritic arborization. It also controls the activity of many genes, including p300, NF-kB, and c-Myc. Notch ligands have differing effects on dendritic cell differentiation. Researchers are currently trying to find co-activators for Notch receptors that have the greatest impact on brain development.
Type I transmembrane type 1 receptors have an extracellular and intracellular region. The intracellular region also contains an extracellular area containing a variable amount of EGF-like repetitives and a N terminal domain containing three cysteinerich tandem Lin12/Notch Repeat (LNR) domains. In addition, the Notch receptor has a DSL domain, a cytoplasmic RAM23 domain, six Ankyrin repeats, and two nuclear localization signals.
Many aspects of the Notch pathway could lead to new options in the prevention and treatment of gastric cancer. Gastric cancer cells are dominated, as previously mentioned, by parietal cells and zymogenic cell. Gastric carcinogenesis is promoted by Notch signaling in these cells. It is now clear that Notch plays a role in gastric cancer. Notch is also involved in the development of many clinical parameters.
A recently approved inhibitor of Notch signaling is expected to prevent BM-mediated drug resistance by making myeloma cells sensitive to chemotherapeutic agents. RO4929097, a compound that has been tested in clinical trials, showed a strong inhibitory effect on Notch signaling. However, RO4929097 failed to stop the proliferation and spread of cancer cells. Instead, it produced a normal phenotype.
The Notch homolog 1 translocation-associated gene (NAM1) has been shown to regulate the expression of genes involved in gastric cancer. NAM overexpression has the potential to block activation of downstream affector genes. This is a promising development in gastric cancer. However, more research is needed to determine the exact role of NUMBL1 as a cancer treatment.
NAMPT may be useful in the treatment of cancer. It inhibits cancer cell proliferation by increasing NAD+ pools, thereby promoting ER Stress and stimulating PARP1/SIRT1 signaling. NAMPT is a promising oncogene. It could be used to treat the disease if it has an effect on cancer cells.
PMID: 8878478 by Joutel A., et al. Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia.
PMID: 10079256 by Gray G.E., et al. Human ligands of the Notch receptor.