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- Table of Contents
OXTR / Oxytocin receptor · Western blot design guide
Design a Western Blot for OXTR
Source-linked OXTR Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-OXTR WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
OXTR Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~42.8 kDa | |
| Observed band | ~43 kDa | |
| Gel | 5–20% (catalog M01566) | |
| Negative control | Suggested KO / knockdown lysate |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Phosphorylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB Hallmark membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked OXTR Western Blot Protocol Options
The M01566 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human Jurkat, human PC-3, human A549, human Caco-2, rat PC-12, mouse RAW264.7 (catalog M01566) |
| Gel % | 5–20% (catalog M01566) |
| Load | 30 ug; reducing conditions (catalog M01566) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M01566) |
| Membrane | nitrocellulose membrane (catalog M01566) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M01566) |
| Primary antibody | M01566 · 1:500 (catalog M01566) |
| Primary incubation | overnight at 4°C (catalog M01566) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:500 (catalog M01566) |
| Secondary incubation | 1.5 hour at RT (catalog M01566) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M01566) |
| Detection | ECL (catalog M01566) |
Section 2
What Is the Expected OXTR Western Blot Band Size?
OXTR is predicted at 42.8 kDa and observed near 43 kDa; the cause of any difference is not established.
| Band near 43 kDa | Matches the empirical OXTR band; confirm identity with appropriate controls |
| Band above 43 kDa | Could reflect N-linked glycosylation, but a shift is not established |
| Broad signal around 43 kDa | Could reflect variable N-linked glycosylation if present |
| Weak or absent band in whole-cell lysate | Membrane-associated OXTR may be poorly recovered |
💡Expected OXTR appearanceOXTR has a predicted mass of 42.8 kDa and an empirical band near 43 kDa; confirm band identity with appropriate controls, since the supplied features do not establish a migration effect.
| Predicted OXTR mass | 42.8 kDa predicted; the empirical band is near 43 kDa |
| N-linked glycosylation site Asn8 | May affect apparent size if occupied; no shift is established |
| N-linked glycosylation site Asn15 | May affect apparent size if occupied; no shift is established |
| N-linked glycosylation site Asn26 | May affect apparent size if occupied; no shift is established |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane-associated OXTR may be poorly recovered | Check membrane extraction and use a known positive lysate |
| Band higher than expected | N-linked glycosylation is possible, but the cause is unproven | Compare matched samples with and without deglycosylation and verify band identity |
| Band lower than expected | The supplied features do not establish a lower-mass OXTR form | Check antibody specificity and compare with a known positive lysate |
| Broad smear instead of sharp band | Variable N-linked glycosylation is possible, but a smear is not established | Compare matched samples with and without deglycosylation |
| Multiple bands | Different glycosylation states are possible; distinct bands are not established | Use band-identity controls and compare matched deglycosylated samples |
| Weak or no signal | Recovery of this multi-pass membrane protein may be low | Check membrane solubilization and use a known positive lysate |
Experiment evidence
Western blot images showing OXTR band
M01566 · OXTR
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for OXTR Western blot
🧪For positive controls for OXTR in Western blot, you can use no HPA-supported sample because the supplied HPA record has no positive candidates.
Positive control: No high/medium HPA tissue identified
Negative control: Suggested KO / knockdown lysate
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: The supplied HPA record has no tissue data, so suitable tissue controls cannot be identified.
HPA tissue expression evidence for OXTR
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No high/medium HPA tissues identified in the supplied evidence. | ||||
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| No lower-expression tissue rows available in the supplied evidence. | ||||
Section 3
Advanced OXTR Western Blot Tips
Deeper troubleshooting and optimisation questions for OXTR, answered from its protein features.
How should OXTR band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could multiple OXTR bands represent isoforms?
Isoforms · The supplied record lists one isoform and no alternative sequence. Do not assign additional bands to OXTR isoforms on this evidence alone.
Which OXTR modifications matter when interpreting bands?
PTM · UniProt lists N-linked glycosylation at Asn8, Asn15, and Asn26, plus phosphoserine at Ser366 and Ser368. These are UniProt sequence coordinates; antibody or paper numbering may differ. The listed sites do not establish that distinct bands will appear.
Does this guide establish induction of OXTR?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for OXTR Western blot?
Transfer · OXTR is a 389-residue multi-pass membrane protein with a predicted mass of 42.8 kDa. Check transfer recovery around the supplied observed position of about 43 kDa. The supplied features do not identify an optimal transfer method.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M01566 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should OXTR band intensity be quantified?
Quantitation · Use the same defined band region across samples, centered on the supplied approximately 43 kDa observation, and apply the same rule if nearby bands appear. The listed modification sites do not establish which additional bands, if any, contain OXTR.
Why might OXTR migrate differently from its predicted mass?
Interpretation · OXTR has a predicted mass of 42.8 kDa, and the supplied observed band is about 43 kDa. UniProt lists N-linked glycosylation sites at Asn8, Asn15, and Asn26, but their presence alone does not establish a visible shift or explain a mass difference.
First compare them with the approximately 43 kDa observed band. OXTR has three listed N-linked glycosylation sites, two phosphoserines, and one disulfide bond, but these features alone cannot identify an unexpected band or prove its cause. The supplied record lists only one isoform.
First compare them with the approximately 43 kDa observed band. OXTR has three listed N-linked glycosylation sites, two phosphoserines, and one disulfide bond, but these features alone cannot identify an unexpected band or prove its cause. The supplied record lists only one isoform.
Boster reagents
OXTR Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Oxytocin Receptor Rabbit Monoclonal Antibody
Cat # M01566
M01566 is a rabbit monoclonal anti-OXTR antibody listed for human, mouse, and rat. Its WB image shows an approximately 43 kDa band in human Jurkat, PC-3, A549, and Caco-2, rat PC-12, and mouse RAW264.7 lysates. Orthogonal validation is not provided.
Which to pick: M01566 is the only listed option. Its WB image provides tested examples across the listed species using 30 µg of reducing lysate per lane and antibody at 1:500. Choose it when those reported contexts fit your experiment.
Source: BosterBio OXTR gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P30559.
- Human Protein Atlas. OXTR tissue expression.
- PMC5376115 — target-verified WB comparison
- PMC11794781 — target-verified WB comparison
- PMC8780485 — target-verified WB comparison