PCSK2 Antibodies

Neuroendocrine convertase 2 (PCSK2)

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells.

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Gene Information

Human
Gene Name:	PCSK2
Uniprot:	P16519
Entrez:	5126

Family

Belongs to:
peptidase S8 family

Alternative Names

EC 3.4.21; EC 3.4.21.94; KEX2-like endoprotease 2; NEC 2; NEC2; NEC2SPC2; neuroendocrine convertase 2; PC2; PC2NEC-2; PCSK2; Prohormone convertase 2; Proprotein Convertase 2; proprotein convertase subtilisin/kexin type 2; SPC2

Molecular Weight

Mass (kDA):
70.565 kDA

Genomic Context

Human
Location:	20p12.1
Sequence:	20; NC_000020.11 (17226107..17484578)

Subcellular Localizations

Cytoplasmic vesicle, secretory vesicle. Secreted. Localized in the secretion granules.

Diseases

• Malignant Neoplasms
• Neoplasms
• Thyroid Neoplasm
• Diabetes Mellitus, Non-insulin-dependent
• Malignant Neoplasm Of Thyroid
• Malignant Paraganglionic Neoplasm
• Diabetes Mellitus
• Obesity
• Adenoma
• Carcinoma
• Pituitary Diseases
• Follicular Adenoma
• Follicular Thyroid Carcinoma

• Thyroid Nodule
• Vaccinia
• Inflammation
• Carcinoma, Papillary
• Insulin Resistance
• Cardiovascular Diseases
• Papillary Thyroid Carcinoma

Pathways

• Secretion
• Insulin Secretion
• Cell Proliferation
• Cell Differentiation
• Pathogenesis
• Glucagon Secretion
• Secretory Pathway
• Lactation
• Pancreas Development
• Gene Silencing
• Parturition
• Cell Development
• Gluconeogenesis

• Energy Homeostasis
• Localization
• Glomerular Filtration
• Glucose Homeostasis
• Hatching
• Regulated Secretory Pathway
• Hatching Behavior

PTMs

• Cleavage

• Phosphorylation

• Amidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PCSK2

Best Uses Of The PCSK2 Marker Antibody

If you're looking for an efficient way to measure PCSK2 protein in your research, then you've probably considered using a Boster Bio high-affinity primary antibody. Boster's antibodies have been validated on Western Blotting, Immunohistochemistry, and ELISA. Their high affinity antibodies, which have been used for over twenty-five years, are highly cited within the research community.

High-affinity primary antibodies

Long-lived plasmacells (LLPCs), which are long-lived, secrete high affinity antibodies. They also mediate humoral memory. However, there have been few studies that examined the associations of LLPCs with affinity and antibody production. The authors immunized channel cats with TNP to study their antibody titers, dynamics, and production. They believed that LLPCs secrete antibodies of high-affinity and that the dynamics associated with serum antibody titers could be explained by the correlation between the affinity of LLPCs and the dynamics of serum antibodies.

The study revealed that LLPCs were present in the anterior kidneys of channel catfish. These cells produce high affinity antibodies and are not evenly distributed in lymphoid tissues. They are located in the anterior renal, a tissue that resembles bone marrow. This discovery has important implications for vaccine design in teleosts. The long-term maintenance and use of high-affinity antibodies may be essential for successful vaccination of teleosts.

Background staining can be reduced by using an antigen affinity purified antibodies. It can also reduce cross-reactivity between primary antibodies. These antibodies must also not cross-react with each other or with sequential antigens. They are not suitable for use if these conditions are not met. And the quality of these antibodies can vary widely between commercial sources. These companies offer assistance and verification. You can always contact these companies if you are unsure whether the antibody you purchased is suitable for use during an experiment.

LLPCs secrete a higher level of serum antibodies than plasma cells, which can help maintain antigenic memories. LLPCs have been called memory-providing and persist long after stimulation with antigen. They also accumulate mutations at the V region. These cells account for over 80% of the antibodies produced in serum. This study shows how important it is to keep high-affinity antibody levels in the later stages of an immune system response.

The KD value of the RabMAb antibody differs from that for the mouse MAb. The KD value of the mouse MAb antibody is different from that for the mouse MAb. This may be due to the fact that it was derived using published literature. All KD values of RabMAb antibodies were derived using the Ol-RD measurement program. The lower the KD the higher the affinity. The concentration of antigen in the sample determines the KD value.

The MBP-Linker scFv antibody has been purified by Ni2+ affinity chromatography. It was then tested for IFN-g affinity using ELISA. The affinity constant was calculated by comparing measured data from four different concentrations. The result was a highly targeted anti-IFN g antibody. The developed immunoassay method also demonstrates that the scFv antibody is highly active in detecting IFN-g in real samples.

The IFN-g protein was used as an immunogen in the immunization procedure. The phages were then placed on separate plates and immunized against the purified IFN–g. The phage ELISA was performed on pre-coated 96 mm ELISA plates. The plates were incubated for 2 h at 37C, washed with BST, and the binding activity of the enriched clones was determined using a phage EISA.

PCSK2 Marker

It is difficult to develop an antibody that is high-affinity for the PCSK2 protein. Several methods are required to assess its sensitivity and specificity. Boster offers several high-affinity primary antibodies that have been validated in ELISA, Western Blotting, and Immunohistochemistry. Boster has antibodies that can detect PCSK2 as well as the phosphorylated version of the protein, PCSK1.