PLVAP / Plasmalemma vesicle-associated protein · Western blot design guide

Design a Western Blot for PLVAP

Source-linked PLVAP Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-PLVAP WB antibodies. Everything you need to plan the experiment before you commit precious samples.

Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for PLVAP: expected band ~50.6 kDa, hero antibody A07024, catalog values and labelled standard workflow; separate PMC comparisons on the guide — Printable **PLVAP** Western blot protocol sheet — expected band ~50.6 kDa, antibody A07024, controls and PMC citations. Open the full PLVAP WB guide →

Navigate to: Protocols Band Controls Antibodies

PLVAP Western Blot Experimental Design Guide

Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	~50.6 kDa
	Observed band	~53 kDa
	Gel	5–20% (catalog A07024)
	Positive control ⓘ	Endometrium (IHC candidate; verify WB) +1 more
	Negative control ⓘ	Adipose tissue (IHC candidate; verify WB)

Important caveats

Reasons your observed band may differ from the expected size.

	PTM	Glycosylated
	Caveat	Modification-state controls
	Gene-set association	MSigDB Hallmark membership
	Isoform	1 isoform(s)

Section 1

Source-Linked PLVAP Western Blot Protocol Options

The A07024 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.

Recommended Western blot protocol parameters
Sample / lysate	human 293T, human HEL (catalog A07024)
Gel %	5–20% (catalog A07024)
Load	30 ug; reducing conditions (catalog A07024)
Transfer	a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A07024)
Membrane	nitrocellulose membrane (catalog A07024)
Blocking	5% non-fat milk/TBS for 1.5 hour at RT (catalog A07024)
Primary antibody	A07024 · 0.5 μg/mL (catalog A07024)
Primary incubation	overnight at 4°C (catalog A07024)
Secondary antibody	goat anti-rabbit IgG-HRP, 1:5000 (catalog A07024)
Secondary incubation	1.5 hour at RT (catalog A07024)
Wash	TBS-0.1%Tween 3 times with 5 minutes each (catalog A07024)
Detection	ECL (catalog A07024)

Section 2

What Is the Expected PLVAP Western Blot Band Size?

PLVAP is predicted at 50.6 kDa and observed near 53 kDa in reducing lysates; the cause of that difference is not established.

What am I looking at on my blot?
Band near 53 kDa	PLVAP band observed in reducing whole-cell lysates
Band near twice the monomer size	possible PLVAP homodimer under conditions that preserve it
Variable migration near the monomer band	possible contribution from N-linked glycosylation; a visible shift is unproven
Weak or absent band in a soluble fraction	PLVAP is a membrane-associated protein

💡Expected PLVAP appearancePLVAP has a predicted mass of 50.6 kDa and an observed band near 53 kDa in reducing whole-cell lysates; the difference is unexplained, so confirm identity with appropriate controls.

How each factor affects band size
Predicted PLVAP mass	50.6 kDa sequence-based reference; the observed reducing-lysate band is near 53 kDa
N-linked glycosylation at Asn83	may affect apparent migration; no site-specific shift is established
N-linked glycosylation at Asn89	may affect apparent migration; no site-specific shift is established
N-linked glycosylation at Asn113	may affect apparent migration; no site-specific shift is established
N-linked glycosylation at Asn151	may affect apparent migration; no site-specific shift is established
PLVAP homodimer	may produce a band near twice the monomer size if the dimer remains intact

Why is my band missing or off?
Situation	Likely cause	Next action
No band in lysate	membrane-associated PLVAP may be poorly recovered	check membrane extraction and include a positive-control lysate
Band higher than expected	a PLVAP homodimer may persist	compare reducing and non-reducing preparations
Broad smear instead of sharp band	heterogeneous N-linked glycosylation is possible but unconfirmed	compare matched samples before and after N-glycan removal
Multiple bands	a retained homodimer or differing glycosylation may contribute	compare reducing conditions and use band-identity controls
Weak or no signal	membrane-associated PLVAP may be underrepresented in the sample	enrich the membrane fraction and verify recovery

Source: UniProt Q9BX97 Human Protein Atlas product datasheet (A07024)

Experiment evidence

Western blot images showing PLVAP band

A07024 · PLVAP

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for PLVAP Western blot

🧪HPA-IHC candidate guidance (verify in WB): For positive controls for PLVAP in Western blot, you can use endometrium tissue, where HPA reports high expression.

Positive control: Endometrium (IHC candidate; verify WB)

Negative control: Adipose tissue (IHC candidate; verify WB)

Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.

⚠️Feasibility: Because PLVAP is membrane-associated, use a lysate that retains membrane proteins.

HPA tissue expression evidence for PLVAP

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Higher expression tissues · candidate positive controls from IHC

Tissue	Cell type	Level	Evidence	Source
Endometrium	endothelial cells	High	Protein (IHC)	HPA →
Colon	endothelial cells	Medium	Protein (IHC)	HPA →

Lower expression tissues · IHC evidence, not confirmed WB-negative controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	adipocytes	Not detected	Protein (IHC)	HPA →
Adrenal gland	glandular cells	Not detected	Protein (IHC)	HPA →
Appendix	glandular cells	Not detected	Protein (IHC)	HPA →
Bone marrow	hematopoietic cells	Not detected	Protein (IHC)	HPA →
Breast	adipocytes	Not detected	Protein (IHC)	HPA →

Section 3

Advanced PLVAP Western Blot Tips

Deeper troubleshooting and optimisation questions for PLVAP, answered from its protein features.

How should PLVAP band migration be interpreted?

Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.

Could PLVAP isoforms explain multiple bands?

Isoforms · The supplied record lists one isoform and no alternative sequence. It therefore provides no isoform-based explanation for multiple bands. Check sample preparation and band specificity before assigning an additional band to PLVAP.

Which PLVAP glycosylation sites matter when interpreting a band?

PTM · UniProt lists N-linked sites at Asn83, Asn89, Asn113, and Asn151, using UniProt sequence coordinates. A glycosidase comparison can test whether glycosylation affects migration; the listed sites alone do not guarantee a visible shift.

Does this guide establish induction of PLVAP?

Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.

How should transfer be checked for PLVAP?

Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.

How should blocking be optimized?

Blocking · Standard workflow guidance: follow the A07024 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.

How should the PLVAP band be quantified?

Quantitation · Quantify the band near the reported ~53 kDa position within the assay’s linear range. Use consistent membrane-protein extraction and sample preparation across conditions, since PLVAP is a single-pass membrane protein and has listed glycosylation sites. Confirm band identity before combining signals from additional bands.

Why is the PLVAP band near 53 kDa rather than 50.6 kDa?

Interpretation · 50.6 kDa is the predicted mass, while ~53 kDa is the reported apparent band. UniProt lists four N-linked glycosylation sites, but those features alone do not establish the cause of the difference. Compare untreated and deglycosylated samples to test whether glycans affect migration.

UniProt describes PLVAP as a homodimer. An upper band could warrant testing for dimer retention, but the record does not establish that a dimer survives Western blot preparation. Compare reducing and denaturing conditions before assigning it.

UniProt places PLVAP at the cell membrane and caveolae, with a perinuclear cytoplasmic location also listed. Include a membrane-containing fraction when assessing recovery, and compare fractions if the expected band is weak or absent.

Boster reagents

PLVAP Western Blot Antibodies

Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.

Real WB data

Western blot analysis of PLVAP using anti-PLVAP antibody (A07024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLVAP antigen affinity purified polyclonal antibody (Catalog # A07024) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLVAP at approximately 53 kDa. The expected band size for PLVAP is at 51 kDa.

Anti-PLVAP Antibody Picoband®

Cat # A07024

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A07024 is a human-reactive anti-PLVAP antibody with a Western blot image showing a band near 53 kDa in human 293T and HEL whole-cell lysates; the expected size is 51 kDa. The supplied evidence is limited to this product image.

Which to pick: A07024 is the only listed option. Its Western blot image uses human 293T and HEL lysates, making it the directly documented choice for those samples; performance in other sample types is not shown.

Source: BosterBio PLVAP gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.

References

UniProt Consortium. UniProt entry Q9BX97.
Human Protein Atlas. PLVAP tissue expression.
PMC4277272 — target-verified WB comparison
PMC11909579 — target-verified WB comparison
PMC9204201 — target-verified WB comparison