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- Table of Contents
Facts about PR domain zinc finger protein 5.
Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue elements, such as HAPLN1, and molecules regulating cell migration and adhesion, such as EDIL3 and TGFB2.
Human | |
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Gene Name: | PRDM5 |
Uniprot: | Q9NQX1 |
Entrez: | 11107 |
Belongs to: |
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class V-like SAM-binding methyltransferase superfamily |
PFM2PR domain-containing protein 5; PR domain containing 5; PR domain zinc finger protein 5
Mass (kDA):
73.09 kDA
Human | |
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Location: | 4q27 |
Sequence: | 4; NC_000004.12 (120684906..120922866, complement) |
Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Nucleus.
The PRDM5 marker is a biological protein that represses transcription and recruits histone methyltransferases. Biological assays commonly use antibodies to detect PRDM5. Most animal samples contain PRDM5 and Boster Bio uses rabbit and mouse to develop their antibodies. PRDM5 is not only important for repressing transcription but also recruits histone methyltransferases, which is crucial for DNA replication and repair.
The PRDM5 protein marker is found in many cells' genomes. It is used for a variety of biological tests. These antibodies can be monoclonal/polyclonal and produced using rabbits and mice as model species. Boster Bio's PRDM5 antibody is based on the suppression of transcription by the PR domain protein zinc finger protein (PRDM5). They have been tested by ELISA, immunohistochemistry, and Western Blotting.
Specificity and affinity are two of the most important characteristics of a primary antibody. The specificity of an antibody is determined by the strength and type of the noncovalent link between it and the target protein. A high affinity primary antibodies is capable of detecting, purifying, measuring, and measuring the target antigen. The PRDM5 marker is used to ensure high-affinity antibody production. The PRDM5 marker can be found in boster bio High affinity primary antibodies using PRDM5.
These antibodies are designed to be used in immunoassays which depend on the ability to bind to antigens. Some of these include Western Blotting, immunohistochemistry, and histopathology. Another type, ELISA or enzyme-linked, immunosorbent assay (which measures the presence and characteristics of specific types of cells), is another type of immunoassay.
Monoclonal primary antibodies are the most widely used in research. These antibodies have high specificity and cross-reactivity for a specific antigen. There is little variation from lot to lot. Monoclonal antigens were traditionally raised in mice to be used for various purposes. However technological advances have allowed them to be made against different species.
PRDM5 contains an antigen that can easily be detected by a wide variety of biological assays. Boster Bio produces polyclonal and monoclonal antibodies that bind to PRDM5 in various animal samples. When developing PRDM5 antibodies, the company uses rabbit and mouse as its model system. The PRDM5 Gene represses transcription. It recruits histone methyltransferases.
Boster Bio PRDM5 membrane staining kits contains antibodies that detect PRDM5 proteins. These antibodies can react with PRDM5 from various animal samples and can be monoclonal as well as polyclonal. Boster Bio's antibody recognizes PRDM5 in rabbits and mice. PRDM5 a zinc finger protein has a PR domain and represses the transcription by recruiting histonemethyltransferases.
Western blotting with a prestained proteomic dye yields clearer visualizations when applied to a PVDF membrane, nitrocellulose or nylon. The PVDF membrane however produced clear bands on the membrane's back due to the slow transfer of proteins. It is important to use different membranes for different immunoblotting procedures. Different membranes are best for different immunoblotting procedures to get the best results.
Boster Bio's protein transfomance by membrane staiing kit can be used to detect the transfer efficiency of proteins in the WB. This kit uses an enzyme conjugated secondary antibody in order to label target proteins within gels. The protein is then transferred onto membranes, which can be made out of nitrocellulose and polyvinylidene trifluoride. Although the nitrocellulose membrane can be used quickly and is cheap, it has some drawbacks. For example, it tends to erase small molecular protein molecules when washed.
The Boster Bio protein transfer efficiency by membrane staing kit includes the following reagents. The first reagent consists of a complete cell lysis buffer. This is particularly useful for the culture and maintenance of mammalian cells. It can be used in protein purification and immunoassays. It does not interfere with biological activity or protein immunoreactivity. The RIPA buffer can be used for Western Blot. It allows primary antibodies to be removed from the membrane without affecting the immobilized antigen. This means that the membrane can easily be reused.
The second reagent detects proteins that have been transferred from a serum to a PVDF membrane or NC membrane. In both cases, the IgG staining required four times less serum proteins than the A2M staining. The intensity of staining was 1.9- to six-fold greater than that on NC membrane. Finally, the NC membrane allowed detection of two targets simultaneously.
Boster Bio uses membrane staing kits to detect specific proteins. The antibodies used were purchased from Vector Laboratories in Burlingame, CA, and Proteintech Group in Chicago, IL. Finally, the samples were placed in TBS for 30 minutes. The protein bands then were visualized using ECL Plus Reagents.
There are two methods that can be used to detect protein. Semi-dry methods use a gel-membrane filter sandwich between filters that contain transfer buffer. The NC membrane's pores are smaller, making it more effective and taking less time than wet. In contrast, the wet transfer method suspends the sandwich vertically in the transfer buffer. The proteins are transferred under an intense electric field.
PMID: 15077163 by Deng Q., et al. PRDM5 is silenced in human cancers and has growth suppressive activities.
PMID: 17699856 by Watanabe Y., et al. PRDM5 identified as a target of epigenetic silencing in colorectal and gastric cancer.