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- Table of Contents
SHMT2 Antibodies
AND SHMT2 ELISA kits, SHMT2 Proteins
Many biological assays use antibodies to detect SHMT2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SHMT2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SHMT2 antibodies...
Designing a Western blot for SHMT2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SHMT2 Western Blot GuideDesigning an IHC experiment for SHMT2? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the SHMT2 IHC GuideSHMT2 Infographic by Boster Bio
All SHMT2 Products
Product
Figures
Specificity
Applications
Price
Anti-SHMT2 Antibody Picoband®
1 Citations
Human, Mouse, Rat
ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # A03233-1
100 μg/vial
Cat # A03233-1
100 μg/vial
SHMT2 Overview
Facts about Serine hydroxymethyltransferase, mitochondrial.
Serine hydroxymethyltransferase, mitochondrial (SHMT2)
Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:29364879, PubMed:25619277). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of a single carbon from serine to tetrahydrofolate (PubMed:25619277).
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is needed to stop uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10- methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base in the wobble position of some mitochondrial tRNAs (PubMed:29452640, PubMed:29364879).
Gene Information
| Human | |
|---|---|
| Gene Name: | SHMT2 |
| Uniprot: | P34897 |
| Entrez: | 6472 |
Family
| Belongs to: |
|---|
| SHMT family |
Alternative Names
EC 2.1.2.1; GLY A+; GLYA; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; serine aldolase; serine hydroxymethylase; serine hydroxymethyltransferase 2 (mitochondrial); serine hydroxymethyltransferase, mitochondrial; Serine methylase; SHMT; threonine aldolase
Molecular Weight
Mass (kDA):
55.993 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 12q13.3 |
| Sequence: | 12; NC_000012.12 (57229685..57234935) |
Subcellular Localizations
Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid. Mitochondrion inner membrane. Cytoplasm. Nucleus. Mainly localizes in the mitochondrion. Also found in the cytoplasm and nucleus as part of the BRISC complex (PubMed:24075985).
Diseases
- Brain Ischemia
- Malignant Neoplasms
- Malignant Neoplasm Of Breast
- 5,10-methylenetetrahydrofolate Reductase Deficiency
- Developmental Delay (disorder)
- Right-sided Displacement Of Abomasum
- Genomic Instability
- Mammary Neoplasms
Interacting Proteins
- ARL6IP1
- CMTM5
- KIRREL3
- MAL2
Pathways
- Methylation
- Dna Methylation
- Dna Repair
- Oocyte Maturation
- Nuclear Import
PTMs
- Sumoylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SHMT2
bosterbio.com/bosterbio-gene-info-cards/SHMT2
References
PMID: 10828359 by Snell K., et al. The genetic organization and protein crystallographic structure of human serine hydroxymethyltransferase.
PMID: 8999870 by Stover P.J., et al. Molecular cloning, characterization, and regulation of the human mitochondrial serine hydroxymethyltransferase gene.