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- Table of Contents
Designing a Western blot for SLC27A2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SLC27A2 Western Blot GuideDesigning an IHC experiment for SLC27A2? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the SLC27A2 IHC GuideFacts about Very long-chain acyl-CoA synthetase.
In vitro, activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving in the de novo synthesis from cholesterol. Doesn't utilize C24 bile acids as substrates.
| Human | |
|---|---|
| Gene Name: | SLC27A2 |
| Uniprot: | O14975 |
| Entrez: | 11001 |

| Belongs to: |
|---|
| ATP-dependent AMP-binding enzyme family |

Very long-chain acyl-CoA synthetase
Mass (kDA):
70.312 kDA

| Human | |
|---|---|
| Location: | 15q21.2 |
| Sequence: | 15; NC_000015.10 (50182196..50236392) |
Expressed in liver, kidney, placenta and pancreas.
Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane; Peripheral membrane protein. Cell membrane; Multi-pass membrane protein. Microsome.


PMID: 10198260 by Steinberg S.J., et al. Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism.
PMID: 10640429 by Smith B.T., et al. Intraperoxisomal localization of very-long-chain fatty acyl-CoA synthetase: implication in X-adrenoleukodystrophy.