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- Table of Contents
Facts about Slit homolog 1 protein.
SLIT1 and SLIT2 together appear to be essential for midline guidance in the forebrain by acting as gruesome signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. .
Human | |
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Gene Name: | SLIT1 |
Uniprot: | O75093 |
Entrez: | 6585 |
Belongs to: |
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No superfamily |
KIAA0813; MEGF4; MEGF4slit (Drosophila) homolog 1; MGC164811; Multiple EGF-like domains protein 4; Multiple epidermal growth factor-like domains protein 4; SLIL1; slit homolog 1 (Drosophila); slit homolog 1 protein; Slit1; Slit-1slit1; SLIT3
Mass (kDA):
167.926 kDA
Human | |
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Location: | 10q24.1 |
Sequence: | 10; NC_000010.11 (96998038..97185959, complement) |
Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
Secreted.
High-affinity primaries are the gold standard for antibody production. They are crucial to your research's success. Boster Bio high-affinity antibody have been cited numerous times and are respected by the scientific community as reliable and quality. They are available in both murine and human forms, and can be validated by Western Blotting or Immunohischemistry.
Next-generation sequence analysis is an exciting way to analyze antibody repertoires. Single-cell sequence is very useful because it allows you to determine paired L-H chains at a large scale. Single-cell sequences also provide a record of evolutionary processes. Computational tools can be used to reconstruct antibody clonal linesages. Crystal structures of affinity-matured antibodies have also provided new insights into the process of antibody maturation.
Next-generation sequencing permits reconstruction of antibody clonal sequences and inferences of germ-line progenitor lines. These sequences might not be identical to true unmutated sequences. It is possible to map the VLJL and VHDJH original junctional sequences with high confidence. However, it is impossible to identify the insertions or deletions that occurred during affinity maturity.
SLIT1 comes from the PGT121 branch, which is split into two evolutionary branches. Both branches are in contact with Asn332 glycocans. They are similar PGT121, in that they contain V3 loops, which are prominent features of the bacterial cell surfaces. The VHFR3 insert allows the antibody to penetrate below the glycan shield.
The germ line precursors of these antibodies don't show detectable binding of recombinant ENV, which raises the question about how these B cells develop. The reconstructed germ-line versions of the affinity-matured antibody antibodies retained V(D)J junctions amino acid sequences. It is possible that non HIV antigens were used to stimulate the initial bNAbs. The Env may cross-react with their resulting intermediate antibodies.
Slit1 Antibody, (G-4) is monoclonal antibody that detects Slit1 proteins of human, mouse, or rat origin. It is available in several conjugated forms as well as non-conjugated. It was designed to detect axonal direction, which is a function of Slit1-3 during normal neural development. It also serves as a high affinity signaling ligand to Roundabout.
Polyclonal antibody, which can recognize any antigen is made from a combination of antibodies derived mainly from B cells with different affinity. Consequently, they may cross-react with irrelevant molecules. Monoclonal antibody are largely IgG immunoglobulins. Secondary antibodies derived predominantly from polyclonal antibodies will be predominantly anti IgG.
The KD (Keule Dissociation Constant) of a monoclonal antibody is measured to determine its affinity for a target. The concentration of the antibody directly affects the KD value. The higher the affinity, the lower the KD. To determine the KD values, Abcam scientists performed data analysis. The resulting data were published in scientific publications.
These primary antibodies are available from different commercial sources, but quality of these products can differ. To avoid wasting precious resources, ensure you read all documentation that comes with your purchase. You should ensure that the quality of the antibody is guaranteed when you purchase from an independent supplier. You can also ask for assistance in the verification of antibodies. You can also order them directly from manufacturers. The commercial source is generally more reliable and can provide better quality antibody.
Different methods are used to prepare the DNA and RNA end of the vectors. This increases the versatility and efficiency in the cloning process. One of these methods uses alkaline phosphatases to remove phosphate groups from DNA and RNA ends. This technique prevents the vector from self-ligating and helps increase the recovery of plasmids containing the desired insert. Another technique is to use calf intestinalphosphatase. This dephosphorylates DNA ends, and prevents self-ligation.
Inducible expression vectors and constitutive expression vectors, are the primary types. Constitutive expression vectors force the transcription of a target gene into many different cell types. Inducible gene expression depends on promoters responding to induction conditions. Some vectors are designed solely for transcription and do not contain sequences for polyadenylation and termination.
Molecular Vectors are DNA molecules that allow you to introduce a gene/protein of interest into a cell. For example, artificial chromosomes in human cells allow for the introduction of huge amounts of DNA. Scientists can use vectors to identify and select DNA sequences within an organism. This makes the process of creating genetically engineered organisms faster and more precise. A vector has many advantages, including the ability improve people's quality of life.
Molecular vectors are either bacterial and viral. They contain a DNA location for insertion and a marker to selectivity, such a antibiotic resistance gene. Bacteria that has plasmids can survive on selective growth mediums. They can also create a gene of your choice. One of the many advantages of plasmids over other types of DNA is their inability to transmit disease. This is an advantage in many ways.
The pBR322plasmid is one of the most widely used cloning genes. Its schematic shows the genes encoded. It also has a restriction site for tetracycline resistance and ampicillin resistance genes. Using this technique, scientists can produce millions of copies of a vector within hours. The vector can then be used for further manipulation once it has been amplified.
Cloning can be achieved using PCR. PCR uses restriction sites to ligate DNA fragments and inserts. The PCR products and a blunt-ended combination cloning process are used. This technique is effective in cloning DNA fragments that don't have restriction sites. This technique allows researchers and scientists to create large genetic pieces and then introduce mutations. These cloning tools have made gene research more efficient.
The expression of the SLIT1 gene has been shown to be AA-selective. It also regulates RBP4 (an AA-selective gene) and PNMA5. The exact mechanism of control for SLIT1's function is not known. However, different mechanisms may be used to regulate the gene in cells. We will review the molecular mechanisms governing SLIT1 gene expression and present a new control method.
SLIT1 knockdown reduces glioma cells invasion and increases radiosensitivity. It was also found that SLIT1 inhibition increased the activity caspase-3 levels in glioma-cells. SLIT1 knockdown significantly inhibited glioma-cell invasion. The resulting tumors were more resistant to radiation than control cells. Further research will be necessary to determine how SLIT1 is involved in the development glioma.
We used biotin-labeled microRNA-640 as a positive control in this study to determine the binding of SLIT1 and this miRNA. This miRNA inhibits the Wnt/b-catenin signaling pathway, which is implicated in radiosensitization. To investigate the role of SLIT1 in radiosensitization, we synthesized miR-640, a biotin-labeled RNA, and transfected cells using Lipofectamine 2000 reagent.
SLIT1 is a marker that is specific for AA. It has been implicated in the dendritic development of cortical neurons in rodents. Whitford et al. Whitford et.al. have shown that SLIT1 can also stimulate the proliferation CNS progenitor cell cells. SLIT1 can be used to study cellular neuronal tumors.
AAV1-MBD4-MBD4-selective gene is expressed in the AAV1-MBD4-MV-MBD4 ectopically. These cells don't have the SLIT1 genes. It is however injected in V1 and PFC. Four weeks later, eGFP expression and RBP4 expression was seen in the injected areas.
We also know that SLIT1 levels are upregulated in many types glioma cells. Knockdown of SLIT1 decreased b-catenin and Wnt proteins, whereas inhibition of miR-640 increased p-GSK-3b levels. This inhibitory effect of SLIT1 knockdown partially reversed the effects of SLIT1-knockdown in glioma cells and improved radiosensitivity in these cells.
PMID: 9813312 by Itoh A., et al. Cloning and expressions of three mammalian homologues of Drosophila slit suggest possible roles for Slit in the formation and maintenance of the nervous system.
PMID: 9693030 by Nakayama M., et al. Identification of high-molecular-weight proteins with multiple EGF- like motifs by motif-trap screening.