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- Table of Contents
SSTR2 / Somatostatin receptor type 2 · Western blot design guide
Design a Western Blot for SSTR2
Real validated SSTR2 Western blot protocols, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-SSTR2 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
SSTR2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~41.3 kDa | |
| Observed band | ~41 kDa | |
| Gel | 5–20% (catalog M01689) | |
| Positive control | Cerebellum (IHC candidate; verify WB) +4 more | |
| Negative control | Caudate (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | Glycosylated + Phosphorylated | |
| Caveat | Modification-state controls | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 2 isoform(s) |
Section 1
Real Curated SSTR2 Western Blot Protocols
The M01689 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human K562, human SH-SY5Y, human Jurkat (catalog M01689) |
| Gel % | 5–20% (catalog M01689) |
| Load | 30 ug; reducing conditions (catalog M01689) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog M01689) |
| Membrane | nitrocellulose membrane (catalog M01689) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog M01689) |
| Primary antibody | M01689 · 1:1000 (catalog M01689) |
| Primary incubation | overnight at 4°C (catalog M01689) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:500 (catalog M01689) |
| Secondary incubation | 1.5 hour at RT (catalog M01689) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog M01689) |
| Detection | ECL (catalog M01689) |
Section 2
What Is the Expected SSTR2 Western Blot Band Size?
SSTR2 is predicted at 41.3 kDa and observed at ~41 kDa; the cause of the small difference is not established.
| Band at ~41 kDa | matches the empirical SSTR2 band; confirm identity with antibody controls |
| Band near twice the monomer size | could reflect a homodimer that survives sample preparation |
| Higher or diffuse band | could reflect variable N-linked glycosylation at Asn9, Asn22, Asn29, or Asn32; a visible shift is not established |
| Additional bands at different positions | could reflect isoforms A and B if their migration differs; distinct bands are not established |
💡Expected SSTR2 appearanceSSTR2 is predicted at 41.3 kDa and observed at ~41 kDa in a reducing Western blot; confirm band identity with appropriate antibody controls because its annotated features do not establish a migration shift.
| UniProt predicted mass | 41.3 kDa predicted; the empirical SSTR2 band is ~41 kDa |
| N-linked glycosylation at Asn9, Asn22, Asn29, and Asn32 | could alter apparent migration if these sites carry glycans; the size effect is not established |
| Homodimer formation | could produce a higher band if the complex survives sample preparation |
| Splice isoforms A and B | could migrate differently, but their relative sizes and separation are not supplied |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | SSTR2 is a multi-pass membrane protein and may be poorly recovered | Check membrane protein extraction and use a positive control lysate |
| Band higher than expected | N-linked glycosylation or a surviving homodimer could affect migration | Compare treated and untreated samples and check reducing preparation conditions |
| Band lower than expected | An isoform or protein breakdown could account for a lower band, but neither is established | Check antibody specificity with an SSTR2 depletion or knockout control |
| Broad smear instead of sharp band | Variable N-linked glycosylation could contribute | Compare with a deglycosylated sample and an SSTR2 identity control |
| Multiple bands | Isoforms A and B or surviving receptor complexes could contribute | Compare reducing conditions and verify bands with an SSTR2 depletion or knockout control |
| Weak or no signal | Membrane protein recovery may be low | Check extraction and loading with a membrane protein control |
Experiment evidence
Western blot images showing SSTR2 band
M01689 · SSTR2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SSTR2 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for SSTR2 in Western blot, you can use cerebellum tissue, where HPA reports high expression.
Positive control: Cerebellum (IHC candidate; verify WB)
Negative control: Caudate (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain such as Ponceau alongside the samples.
⚠️Feasibility: SSTR2 is mainly at the cell surface but internalizes after agonist stimulation, so sample conditions may affect its distribution.
HPA tissue expression evidence for SSTR2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | cells in granular layer | High | Protein (IHC) | HPA → |
| Adipose tissue | adipocytes | Medium | Protein (IHC) | HPA → |
| Adrenal gland | glandular cells | Medium | Protein (IHC) | HPA → |
| Appendix | glandular cells | Medium | Protein (IHC) | HPA → |
| Bronchus | respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Caudate | glial cells | Not detected | Protein (IHC) | HPA → |
| Heart muscle | cardiomyocytes | Not detected | Protein (IHC) | HPA → |
| Hippocampus | glial cells | Not detected | Protein (IHC) | HPA → |
| Lymph node | germinal center cells | Not detected | Protein (IHC) | HPA → |
| Prostate | glandular cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced SSTR2 Western Blot Tips
Deeper troubleshooting and optimisation questions for SSTR2, answered from its protein features.
How should SSTR2 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Can SSTR2 isoforms A and B produce different bands?
Isoforms · Isoform B replaces canonical residues 332–369 with a different sequence. Compare isoform-specific expression or sequence coverage before assigning distinct bands to A and B; sequence variation alone does not establish their apparent masses.
Could glycosylation affect SSTR2 migration?
PTM · UniProt lists N-linked glycosylation at Asn9, Asn22, Asn29, and Asn32. Compare paired untreated and deglycosylated samples to test whether glycosylation affects migration; the listed sites alone do not predict a band shift.
UniProt lists phosphoserines at canonical positions 341, 343, and 348, and phosphothreonines at 353 and 354. These positions fall within the segment replaced in isoform B, so do not assign the same site numbers to B. Their presence does not establish a visible shift.
UniProt lists phosphoserines at canonical positions 341, 343, and 348, and phosphothreonines at 353 and 354. These positions fall within the segment replaced in isoform B, so do not assign the same site numbers to B. Their presence does not establish a visible shift.
How should agonist stimulation affect SSTR2 sampling?
Induction · SSTR2 is mainly at the cell surface under basal conditions and internalizes to the cytoplasm after agonist stimulation. Sample matched conditions and record whether the blot uses total lysate or a membrane fraction; a change in membrane signal need not indicate a change in total SSTR2.
How should transfer be checked for SSTR2?
Transfer · Standard workflow guidance: verify transfer efficiency for the intended target size before interpreting a weak signal. Use total-protein assessment and optimize transfer for the membrane, gel and apparatus; the labelled catalog values take precedence.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the M01689 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should membrane and cytoplasmic SSTR2 signals be quantified?
Quantitation · Quantify matched fractions separately and compare them with total lysate, because agonist stimulation redistributes SSTR2 from the cell surface to the cytoplasm. Keep sampling conditions consistent across comparisons.
Why does SSTR2 appear near 41 kDa?
Interpretation · The observed band near 41 kDa agrees with the predicted 41.3 kDa. Listed modifications alone do not establish a visible shift or explain a mass difference.
Consider isoforms A and B, the four listed N-linked glycosylation sites, and the five listed phosphorylation sites. UniProt also reports SSTR2 homodimers and heterodimers with SSTR3 or SSTR5. These features suggest possibilities, but band position alone cannot identify a modification, isoform, or dimer.
Consider isoforms A and B, the four listed N-linked glycosylation sites, and the five listed phosphorylation sites. UniProt also reports SSTR2 homodimers and heterodimers with SSTR3 or SSTR5. These features suggest possibilities, but band position alone cannot identify a modification, isoform, or dimer.
Boster reagents
SSTR2 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-Somatostatin Receptor 2 SSTR2 Rabbit Monoclonal Antibody
Cat # M01689
The catalog reports one anti-SSTR2 rabbit monoclonal antibody, M01689, with reported human, mouse, and rat reactivity. Its supplied Western blot image shows a band near 41 kDa; no publication evidence is supplied.
Which to pick: M01689 is the only listed option. Its Western blot image includes human K562, SH-SY5Y, and Jurkat cell lysates, plus rat and mouse brain lysates, tested under reducing conditions at 1:1000. Match your sample to these documented contexts.
Source: BosterBio SSTR2 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry P30874.
- Human Protein Atlas. SSTR2 tissue expression.
- PMC8615514 — target-verified WB comparison