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- Table of Contents
Facts about Synaptonemal complex protein 3.
Required for normal meiosis during spermatogenesis and male fertility (PubMed:10678170). Plays a lesser role in female fertility (PubMed:10678170, PubMed:12004129).
Mouse | |
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Gene Name: | Sycp3 |
Uniprot: | P70281 |
Entrez: | 20962 |
Belongs to: |
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XLR/SYCP3 family |
SCP3; COR1; MGC71888; SCP3 chromosome 3 open reading frame 8; SCP3 COR1; SCP3; SCP-3; SYCP3; synaptonemal complex protein 3
Mass (kDA):
29.347 kDA
Mouse | |
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Location: | 10|10 C1 |
Sequence: | 10; |
Detected in oocytes (PubMed:16717126). Detected in spermatocytes and testis (at protein level) (PubMed:10678170, PubMed:16717126, PubMed:22761579).
Boster has made submissions of results from special species and sample studies easy for scientists to get credit. This service allows researchers from all parts of the world to submit their results, and receive product credits. Scientists can also submit their species and application results to receive credit for their work. The SYCP3 marker can be used for all species types.
Boster Bio catalog number: RP1035 Anti-SCP-3 Monoclonal Antibody. This antibody is for WB applications. It reacts well with Human. The antibody is stable at -20°C and can be stored for up to one year. It comes in a single aliquot that contains 5 mg bovine serum albumin as a dilutive. Depending on the length of the immunogen, the price may vary.
To form oligomers or sub-visible aggregates, peptide tags can be used. These tags are found on Dengue virus serotypes 3, 4 and 5. The D3C5X/D4C5X tags have 5-Aspartic amino acids at the C-terminus. The same amino acid sequences are found in the D4C5D+D4C5K tags.
The five-residue SCP-tag sequence is attached to the C-terminus of D3ED3 or D4ED3. The Glycines in the tag act as spacers between the host protein and the SCP tag. This makes Anti-SCP-3 Boster Bio extremely potent. It is highly effective against many types of tumor cells, including melanomas.
The SCP3 marker is used in molecular biology in many ways. SYCP3 identified chromosomes moving rapidly in the nucleus at meiotic progression in one study. These tagged proteins may be associated with endogenous SYCP3 or may function independently. SYCP3 can also be a component of synaptonemal complex (SC).
SYCPC is substituted for SYCP3 in males. Its localization in E16.5 oocytes was not axial and was confined to the later stages of chromatin. CSYCP on the other hand showed axial location and covered a large portion of axes visualized via anti-REC8. SYCPC is therefore a possible replacement for SYCP3.
The anti-SCP1 antibody binds with SYCP3 (a marker for spermatocytes) to detect SCs. Spermatocytes contain lateral elements separated by 200nm. They measure 37.1 nm in width and 100 nm in depth. The antibodies are specifically designed for detection of SCs in human fertilized eggs. Recently, the SYCP3 mark has received a lot of attention in these studies.
However, in embryonic growth, the SCP1 gene is required to form transverse filaments in the spermatocytes. Sycp3-knockout mice have spermatocytes containing the SCP1 transverse fibril protein. It forms irregular fibers. Although this study doesn't address the molecular mechanism, the results confirm the previously described role for SCP1 in early embryonic development.
Anti-SCP1 antibodies have been produced for years. Their lateral-element SYCP1 (mRNAs) have been used for studying embryonic stem cells development. The SCP3 marker can be used to visualize transverse filaments. This marker is widely applicable and has a wide range. Its lateral location is very useful in studying the structure of embryonic and adult stem cells.
It was also possible for anti-SCP1 to detect telomeres. Anti-SCP1 antibodies can also be used to detect chromosome ends where SCP3 is not effective. Anti-SCP1 antibodies will prove to be very useful for embryonic stem cell analysis in the future. The SCP3 gene is regulated in the course of development.
SYCP3 is found in many tissues, such the adrenal glands. Researchers can use SCP3/SYCP3 antibodies to study DNA damage response at somatic and XY bodies. Researchers can also use SYCP3 to investigate the role Senataxin plays in gene silencing. This antibody can also help researchers find ectopic germcells in embryonic glands.
SYCP3 is a highly expressed secondary functional member of Cor1/Xmr in the testes. Aarabi et al. The abundance of SYCP3 was found in the testes of bovines. They suggested that it might play a role both in male fertility as well as in testis development. In addition, Wang et al. Wang et.al. also studied the SYCP3 protein and discovered that it had a tetrameric DNA binding domain at both ends.
The SYCP3 indicator is a commonly used antibody to detect RPA. Interestingly, it is also useful for identifying DSBs. This marker is colocalized to RPA2 (markers of DSBs) and RAD51 (markers of transiently formed D loops). However, it has a limited role in predicting meiotic recombination. Anti-RPA antibodies against SYCP3 detected DSBs that had not been repaired in the current study.
The SYCP3 marker is used to detect ectopic germ cells in human embryonic adrenals and to highlight species-specific differences in early gametogenesis. Antibodies to SCP3 can also be used to examine the role played by the endocannabinoid systems in germ cells. VEGF is also important for germ cell survival. Its ubiquitin-like domains have been associated with proliferative and other cells.
SYCP1-SYCE3 are found in wild-type spermatocytes at synapses and aligned ADs. SYCP1 is a key component of meiosis. Both proteins are essential for the alignment of homologs and can be detected using immunofluorescence analysis. SYCE3 and SYCE1 protein localize to synapses. However, in the absence of the latter SYCP1 or SYCE1 expression is widely distributed in spermatocytes.
SYMPK is required for meiotic recombination. It is not easy to determine the role of SYMPK within meiosis. Nevertheless, meiosis depends on the SYMPK genetic product. Anti-RPA uses are possible for the SYCP3 marker. This is a new tool that allows us to explore the function of meiosis.
The SYCP3 mark is a powerful tool in diagnosing RAD51 mutated cellular cells. The SYCP3 protein is a strand invasion inhibitor. It binds RAD51 at high affinity, but is weakly bound to DMC1. Co-expressed with DsDNA, the SYCP3 marker can also inhibit strand invasion.
The SYCP3 proteins bind to the RAD51 genes and inhibit homologous recombination. The SYCP3 gene competes with HOP2-MND1, a second protein in the RAD51 compound. This competition blocks strand invasion because it interferes with the helical grooves on RAD51, and the DMC1 postsynaptic filament.
For this assay, the mutant protein SYCP3 KR-A exhibits reduced RAD51 binding, but retains similar dsDNA binding capacity. The SYCP3 marker was coexpressed with dsDNA for 5 minutes. The D-loop formation rate was measured using agarose gel electrophoresis.
SYCP3 protein plays an important role in meiotic and recombination. Although the mechanism for this regulation is not known, it regulates the strand-invasion activity of recombinases. HOP2-MND1, a Signaling Protein that activates both RAD51 & DMC1, is also a competitor of the SYCP3-MND1 protein.
PMID: 9133341 by Klink A., et al. The mouse synaptosomal complex protein gene Sycp3 maps to band C of chromosome 10.
PMID: 11311943 by Botelho R.J., et al. The genomic structure of SYCP3, a meiosis-specific gene encoding a protein of the chromosome core.