TIA1 Antibodies

Nucleolysin TIA-1 isoform p40 (TIA1)

Involved in other pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte cells.

May be involved in apoptosis. .

Gene Information

Human
Gene Name:	TIA1
Uniprot:	P31483
Entrez:	7072

Family

Belongs to:
No superfamily

Alternative Names

cytotoxic granule-associated RNA-binding protein; nucleolysin TIA-1 isoform p40; p40-TIA-1 (containing p15-TIA-1); p40-TIA-1; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA1 cytotoxic granule-associated RNA binding protein; TIA1 cytotoxic granule-associated RNA-binding protein; TIA-1

Molecular Weight

Mass (kDA):
42.963 kDA

Genomic Context

Human
Location:	2p13.3
Sequence:	2; NC_000002.12 (70209444..70248793, complement)

Subcellular Localizations

Cytoplasm, Stress granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.

Diseases

• Lymphoma
• Neoplasms
• T-cell Lymphoma
• Malignant Neoplasms
• Diffuse Large B-cell Lymphoma
• Ki-1+ Anaplastic Large Cell Lymphoma
• Hodgkin Disease
• Epstein-barr Virus Infections
• Transient Ischemic Attack
• Peripheral T-cell Lymphoma
• Skin Neoplasms
• B-cell Lymphomas

• Inflammation
• Leukemia
• Malignant Paraganglionic Neoplasm
• Lymphoproliferative Disorders
• Virus Diseases
• Lymphoma, T-cell, Cutaneous
• Nose Neoplasms

Interacting Proteins

• SNRNP70
• SNRPA
• SNRPC

Pathways

• Translation
• Pathogenesis
• Immune Response
• Localization
• Cell Death
• Cell Proliferation
• Transport
• Programmed Cell Death
• Response To Oxidative Stress
• Response To Stress
• Dna Repair
• Hypersensitivity
• Inflammatory Response

• Nuclear Import
• Cell Growth
• Angiogenesis
• Rna Processing
• Secretion
• Cell Adhesion
• Dosage Compensation

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation

• Biotinylation
• Oxidation
• Methylation

• Ubiquitination

Research Areas

• Adaptive Immunity
• Apoptosis
• Immunology

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TIA1

Boster Bio: Best Uses Of The TIA1 Marker

Boster offers a high affinity, mouse monoclonal antibody called TIA-1. This antibody is used to detect intracellular T-cell-restricted antigen. For product credits, scientists worldwide can submit their findings. Boster is a member of the Association of Immunologists and Associated Professionals (AIPAC), and their products can be used for both human and animal research.

Boster has high-affinity prima antibodies

In order to produce antibodies against the TIA1 marker, a method called affinity purification is used. Multiple washes are performed on one or more of the antibodies. Each wash is performed after the antibody has been contacted with a cell, biological sample, or extracellular medium. This allows the antibody's reuse for future research. After washing, one of the antibodies is re-conjugated again with a marker protein or target cells.

TIA-1 is a monoclonal mouse antibody

T cells produce TIA-1, a type if antigen. It is a 17kDa cytoplasmic-granule-associated protein that is found on the surface many cells. It is also known under the name GMP-17, or granule-membrane protein of 17kDa. TIA-1 and TIAR are closely related. TIA-1 has the ability to bind cell membranes. It may also be involved with Fas (CD95),-mediated apoptosis. The molecule can be found in more than 90 percent CD16+, fifty to sixty percent CD8+, and less that ten percent of CD4+.

Studies have shown that TIA-1 can be used to identify lymphocytes with cytolytic capabilities. It can also distinguish LGL and other types t-cell leukemias from it, including T cell lymphomas. Researchers also have used TIA-1 as a label for tumor-infiltrating lymphocytes to study the immune response to various types of malignancies.

It is used in the detection of intracellular T-cell-restricted antigens

TIA1 is an immunoglobulin G-domain protein that recognizes T cell-restricted intracellular antigens. TIA1 can also limit cellular translation by binding specific mRNAs. It can also prevent the translation of mRNAs during stress. These properties make TIA1 an excellent immunodiagnostic tool to detect intracellular antigens in T-cell-restricted cells.

TIA1 associates to the 48S Complex, which is made up of eIF4A/eIF4G/eIF4A/eIF3B. This complex also contains TIA1 as well as the small subunit ribosome. TIA1 serves as a cofactor to these proteins and aids in their detection.

Numerous studies have implicated TIA1 Mice have shown that knocking down TIA1 results in decreased cell proliferation and tumor-growth. TIA1 is a protein which is induced by tetracycline. This results in a decrease in cell proliferation. Ectopic expression of TIA1 abolishes endogenous levels, partially represses global translation, and results in a caspase-dependent cell death.

In this study, a number of T-cell-restricted mRNAs were amplified from the IP material. These mRNAs were used in the verification of TIA-1 target genes. Nontarget mRNAs were also detected, such as the housekeeping genes. TIA1 allows you to detect intracellular T-cell-restricted antigen.

The UniGene database allowed us to identify a variety of motifs by comparing the sequences in the experimental data. The highest relative number hits was for the motif containing between 30 and 37 nt. The motif logo shows the frequency of nucleotides aligned in three-dimensional sequences. It was identified as a T cell-restricted intracellular Antigen Motif by analyzing RNA sequences with TIA-1.

TIA1 - A nuclear RNA-binding protein which regulates both alternate and constitutive splicing It promotes exon exclusion and exon inclusion. This protein is recruited from the spliceosome to bind weakly conserved consensu sequences. It has been implicated with the regulation of apoptosis.

T cells produce TIA1, a 17-kDa protein. It is also known for GMP-17. It is expressed on cells with cytolytic activity and could be involved Fas(CD95)-mediated death. 2G9 is a monoclonal anti-TIA1 antibody. It reacts with most monocytes, activated NK cell clones, and thymocytes, but is not reactive with B lymphocytes.

Various types of cancers can increase the expression level of TIA1. However, in tumor cells, it promotes anchorage-dependent and anchorage-independent cell proliferation. This has important implications for cancer immunotherapy. This immune-regulating proteins can also be used for cancer diagnosis and treatment. A tumor cell lacking TIA1 may be more susceptible for invasive disease.

Mutations in TIA1 have been shown to alter biophysical property. Enhanced liquid-liquid separation is essential for stress granule formation. This mutation promotes TDP43 formation in TIA1-positive stressed granules. It also has biological consequences that are not yet clear.