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- Table of Contents
Facts about Mitochondrial import receptor subunit TOM70.
.
Human | |
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Gene Name: | TOMM70 |
Uniprot: | O94826 |
Entrez: | 9868 |
Belongs to: |
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Tom70 family |
FLJ90470; KIAA0719; mitochondrial import receptor subunit TOM70; Mitochondrial precursor proteins import receptor; TOM70; Translocase of outer membrane 70 kDa subunit; translocase of outer mitochondrial membrane 70 (yeast) homolog A; translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae); translocase of outer mitochondrial membrane 70 homolog A (yeast)
Mass (kDA):
67.455 kDA
Human | |
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Location: | 3q12.2 |
Sequence: | 3; NC_000003.12 (100363431..100401089, complement) |
Mitochondrion outer membrane; Single-pass membrane protein.
When it comes to primary antibodies, Boster has been a name trusted in the research community for decades. Boster scientists validated its antibodies using known positive as negative samples on several platforms. Boster antibodies are known for their high affinity, specificity, and high affinity. Boster even offers product credits to the first reviewer who reviews a product. This way, scientists around the world can help validate Boster antibodies for use in their experiments.
Boster Bio has been producing high-affinity prim antibodies for over two decades. Boster Bio has invested in technology and methods to improve its products, which are now used in over 29,000 publications. All of Boster's antibodies have been validated for use in ELISA, immunohistochemistry, and Western Blotting. Boster stands behind its quality guarantee with its Boster Q Guarantee.
The company is focused on the production of high quality monoclonal and multiclonal antibodies that can be used in research. The primary antibodies are compatible for use in many species of plants and are cited in thousands. Their antibodies are trusted by the research community and are regularly cited. The company has been recognized as a major supplier of plant science antibody and has received awards to recognize innovation in plant-based immuneassays.
Researchers trust Boster’s primary antibodies due to their high quality and reliability. CiteAb's data is based on the analysis of hundreds of thousands of antibody citations. The company's quality initiative is designed to address the urgent customer demand for reliable, high-quality antibodies. It is supported and supported by a strong scientific staff that has a proven track record of meeting high-quality standards in research antibodies.
Rockland also produces a range of conjugated primary antibody. These antibodies allow researchers to analyze targets without the need for secondary antibodies. They can be used for immunoblotting, ELISA, flow cytometry, and immunohistochemistry. They are also suitable for many other immunological methods such as Western blotting. The primary antibodies manufactured by the company are very specific and high in titer.
There are two common methods to detect antibodies in western blots. Secondary antibodies are conjugated enzymes to secondary antibodies and are then reacted with a substrate for a signal. The resulting image can then be quantitatively analyzed using colorimetric or densitometry methods. Direct labeling analyses are faster than secondary antibodies because they eliminate the need to use secondary antibodies.
Fluorescent immunoassays provide an additional method of detecting antibodies using western blot. Fluorescent immuneassays use an antibody labeled by a fluorescent color. The dye is excited using a light source that emits transient light. Specialized readers can detect the light at higher wavelengths. This method is widely used as it is highly sensitive and takes less time.
There are three basic methods for detecting antibodies on western blot experiments. The first is gel electrophoresis, which separates proteins by molecular mass. Once the proteins are separated, they are transferred onto membranes. After the proteins have been separated, the membrane is blocked using a protein block buffer and probed with primary antibodies. Secondary antibodies are then conjugated with a reporter molecule, and used to visualize a protein of interest. Both methods require different equipment. One method requires xray films, while another uses a fluorescent camera.
Multiplexing techniques are becoming more common. This allows researchers to use multiple antibodies on a single Western-blot membrane, without having to remove the proteins. Multiplexing is a process that requires specialized equipment. Multiplexed immuneassays are extremely beneficial, as different secondary antibodies emit different wavelengths. These are often used in scientific studies. Multiplexing has many benefits.
Picokine(tm), a high sensitivity ELISA platform, increases the sensitivity of ELISA kit to the picogram range. Picokine(tm), a high sensitivity ELISA platform, validates ELISA kit for a variety different sample types. Images as well as the validation procedure are available upon request. Picoband's secondary antibody, a polymer-based polymer, can be used in place of IHC for up to 30 minutes. The Picoband's design insights are backed by technical support provided by Sanbio, a BeNeLux distributor.
This innovative system allows proteins without any transfer step to be transferred from polyacrylamide jelly to membranes. Proteins that are not transferred are indicated with bands with an irregular appearance and minimal or no staining on membranes. Proteins that have uneven bands or very little staining indicate unequal loading. This method can also detect proteins not transferred, which is a key component to western blotting.
The NC membrane used is either polyvinylidene dfluoride or nitrocellulose. The first has the advantage that it produces little non-specific staining, is easy to use, and is inexpensive. It has a few drawbacks, including the inability to wash out small molecular-protein molecules. The nitrocellulose membrane is fragile, and can lose small molecular substances during the process of transfer.
The primary antibody should be diluted with the Antibody Diluent Buffer according to the manufacturer's protocol. To remove any antibodies still attached to the membrane, incubate the film at 37°C for three days. After that, wash the film three times with TBS Wash Buffer. The second antibody should then be mixed with a dilute sample of the primary antibody along with the Boster4X Dual Color Protein Loading Bulffer. The membrane should be incubated in darkness during this step.
ECL chemistry enables researchers to detect protein expression within biological samples with high sensitivity. This method is able to detect protein expression in multiple targets at once, unlike other chemiluminescent detectors. It has many advantages, including excellent multiplexing ability, low background, high sensitivity, and excellent multiplexing. Despite these advantages, there are still some drawbacks to this method, which researchers should know before purchasing one.
Choosing the right substrate is critical. There are three main types, acridan, luminol, or 1,2-dioxetane of chemiluminescent HRP reagents. The most popular reagent is luminol. It has a short half life of approximately one minute. The others have longer half lives that can last for several hours. In either case, multiple measurements may need to be taken for a single sample.
Boster Bio ECL chemiluminescent detection devices use a special enzyme called HRP. This enzyme is often used in western blot applications. These substrates differ in sensitivity and intensity. This enzyme is best suited to western blot applications that contain a large amount of proteins. Boster Bio ECL is compatible with many western-blotting applications and includes multiple substrates.
ECL chemiluminescent detection devices are versatile and easy-to-use. Its C-DiGit blot scanner eliminates the need for darkroom expenses and developing reagents. It can also be used in conjunction with other imaging systems, such as Western Blots, plate-based Assays, and excised Organs.
DAB chromogenic detecting system allows researchers the ability to examine samples in order to determine if there is apoptosis (DNA damage), or bacterial growth. These dye-based stains are useful in determining whether your sample is alive or dead. DAB-chromogenic detection systems are easier to use than fluorescent methods, and require less preparation. However, it is more difficult than fluorescent methods. Both biotin and non-biotin-based chromogenic detection systems utilize different reagents to achieve their desired results.
PMID: 18331822 by Kato H., et al. Identification of Tom5 and Tom6 in the preprotein translocase complex of human mitochondrial outer membrane.