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- Table of Contents
Facts about Torsin-1A-interacting protein 1.
Binds to A- and B-type lamins. Potential role in membrane attachment and assembly of the nuclear lamina.
Human | |
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Gene Name: | TOR1AIP1 |
Uniprot: | Q5JTV8 |
Entrez: | 26092 |
Belongs to: |
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TOR1AIP family |
lamina-associated polypeptide 1B; torsin A interacting protein 1
Mass (kDA):
66.248 kDA
Human | |
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Location: | 1q25.2 |
Sequence: | 1; NC_000001.11 (179882042..179920077) |
Expressed in muscle, liver and kidney.
Nucleus inner membrane; Single-pass membrane protein.
The TOR1AIP1 marker is a gene which controls and alters topologic DNA states during transcription. It is also used in the detection of protein transfer efficiency and the recording of test results using autoradiography film. You are interested in finding out more? Continue reading. This gene has many benefits. It is used in many different applications including research and diagnostics as well as disease modelling.
Understanding the relationship between the microbiota and the human host is a novel way to study evolution and human health. Boster's infographics give basic information about each of the genes, and the search tool makes it easy for you to find the gene that interests you. The gene informationgraphics include both textual and graphical descriptions of every gene in the human and mouse genomes.
This gene encodes DNA topoisomerase enzyme. It is responsible for controlling the topologic condition of DNA during transcription. Alternative splicing is the process that produces it. This results in many different transcript variants. The best use of TOR1AIP1's gene is research into its role in DNA restoration.
Recent research by four authors examined the evidence for its involvement in cancer. They also noted the importance this gene plays in the development and progression of many types of tumors. These researchers also found a strong association between TOR1AIP1 Marker gene expression and breast cancer risk. A new study suggests that the gene may play an even larger role than previously believed.
Plants can resist pathogens by creating complex structures that block the immune response. This allows the TNL-receptor to form a Tetramer directly by binding to NADase. The BB loop then allows NADase's downstream immune responses to be activated. There are many gene families that encode the protein, including C-JID (Cterminal jelly-roll, Ig-like domain), HD1(helical domain 1) and RPP1AIP1XOPQ1Marker.
EHEC, or enterohemorrhagic Escherichia coli, is a disease caused by a virus that disrupts cell translation. Despite these limitations, the TOR1AIP1Marker Gene has a number beneficial functions, including protecting the kidneys from severe infection. These studies confirm that EHEC can have several antiviral functions.
To determine the protein transfer efficiency, a membrane must have different pores according to the molecularweight target protein. Small molecularweight proteins can be transferred best with membranes 0.45um and 0.2um. PVDF membranes should only be used for proteins that are more than 20kD. This membrane has higher affinity, sensitivity, and resolution.
You need to know the dynamic range of the target protein and the loading control proteins in order to quantify them. If the target protein bands intensities are not in the same linear range, it is difficult to calculate their values. As a loading control, a total proteins stain can be used. After detecting the target proteins, wash the membrane three times with TBS Buffer. Apply a secondary antibody and incubate at 4degC overnight.
The Pierce(tm), G2 Fast Blotter is an excellent example of a protein-transfer apparatus. The high-current power supply combined with the ionic strength buffer increase the transfer efficiency. Semi-dry transfer is faster and more efficient. Wet transfer suspends the sandwich vertically in a buffer. The high-frequency electric fields enable proteins to be transferred at great speed.
This method allows you to check the status of the TOR1AIP1 genetic gene using test results. The test can be carried out using autoradiography films. After recording the results, a luminometer can be used to interpret them. Place the sample comb carefully to avoid creases and bubbles. The film should stay at 37°C for 30-60minutes. The film should then be dried and stored in a darkroom. The protein sample can then be added to a 4X Dual Color Protein Loading Buffer by Boster and allowed it to develop for ten more minutes. The film must then be exposed to both the DAB substrate solution and the BCIP/NBT substrate solution.
PMID: 12061773 by Kondo Y., et al. Molecular cloning of one isotype of human lamina-associated polypeptide 1s and a topological analysis using its deletion mutants.
PMID: 15767459 by Goodchild R.E., et al. The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein.