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- Table of Contents
ZAP70 Antibodies
AND ZAP70 ELISA kits, ZAP70 Proteins
Many biological assays use antibodies to detect ZAP70, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ZAP70 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ZAP70 antibodies...
Designing a Western blot for ZAP70? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ZAP70 Western Blot GuideDesigning an IHC experiment for ZAP70? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ZAP70 IHC GuideZAP70 Infographic by Boster Bio
All ZAP70 Products
Product
Figures
Specificity
Applications
Price
Human
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M00759-2
100 μg/vial
Cat # M00759-2
100 μg/vial
Human, Monkey, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M00766-2
100 μg/vial
Cat # M00766-2
100 μg/vial
Human
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M00757-4
100 μg/vial
Cat # M00757-4
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, WB
$370
Cat # M00754-5
100 μg/vial
Cat # M00754-5
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, WB
$370
Cat # M00767-1
100 μg/vial
Cat # M00767-1
100 μg/vial
Human, Mouse
Flow Cytometry, WB
$370
Cat # M00754-4
100 μg/vial
Cat # M00754-4
100 μg/vial
Human, Mouse
ELISA
$540
Cat # EKC2137
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2137
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse
ELISA
$540
Cat # EKC2136
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2136
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2540
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2540
1 kit, containing two 96-well plates and all necessary reagents
ZAP70 Overview
Facts about Tyrosine-protein kinase ZAP-70.
Tyrosine-protein kinase ZAP-70 (ZAP70)
Tyrosine kinase that plays a vital role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, in addition to thymocyte development.
Contributes also into the activation and development of primary B-lymphocytes. When antigen presenting cells (APC) trigger T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 into the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif in the plasma membrane.
Gene Information
| Human | |
|---|---|
| Gene Name: | ZAP70 |
| Uniprot: | P43403 |
| Entrez: | 7535 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
70 kDa zeta-associated protein; EC 2.7.10; EC 2.7.10.2; mrtle; mur; SRK; SRKFLJ17679; STD; STDFLJ17670; Syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP70; ZAP-70; zeta-chain (TCR) associated protein kinase (70 kD); zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Molecular Weight
Mass (kDA):
69.872 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 2q11.2 |
| Sequence: | 2; NC_000002.12 (97712030..97744327) |
Tissue Specificity
Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
Subcellular Localizations
Cytoplasm. Cell membrane; Peripheral membrane protein. In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity).
Diseases
- Leukemia
- Chronic Lymphocytic Leukemia
- Lymphoid Leukemia
- Neoplasms
- Immunologic Deficiency Syndromes
- Malignant Neoplasms
- Lymphoma
- Cytogenetic Abnormality
- Severe Combined Immunodeficiency
- Autoimmune Reaction
- Tissue Adhesions
- Autoimmunity
- Congenital Combined Immunodeficiency
Interacting Proteins
- CBL
- CD247
- CD3E
- EGFR
- KIT
- LCK
- MET
- PTPN22
- Ubash3b
Pathways
- Cell Activation
- T Cell Activation
- Secretion
- Pathogenesis
- Cell Proliferation
- Cell Development
- Cytokine Production
- Localization
- Immune Response
- Cell Cycle
- T Cell Proliferation
- Cell Death
- Lymphocyte Activation
PTMs
- Phosphorylation
- Dephosphorylation
- Ubiquitination
- Methylation
- Acylation
- Myristoylation
Research Areas
- Adaptive Immunity
- Cell Biology
- Immunology
- Protein Kinase
- Signal Transduction
- Tyrosine Kinases
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ZAP70
bosterbio.com/bosterbio-gene-info-cards/ZAP70
References
PMID: 1423621 by Chan A.C., et al. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain.
PMID: 14985102 by Kuroyama H., et al. Identification of a novel isoform of ZAP-70, truncated ZAP kinase.
1 Publications of Boster ZAP70 Antibodies & ELISA Kits
- PubMed ID: 10.7150/jca.10830, TGF-β Induces Degradation of PTHrP Through Ubiquitin-Proteasome System in Hepat...
*More publications can be found for each product on its corresponding product page