Broad Spectrum Protease Inhibitor Cocktail (100x)

Broad Spectrum Protease Inhibitor Cocktail, For inhibiting proteases and esterase. Cited in 10 publication(s).

Product Info Summary

SKU: AR1182
Size: 1ml

List of Components

DescriptionQuantityVolumeContentsCatalog Number
Broad Spectrum Protease Inhibitor Cocktail11mL (100X)Containing AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A stabilized in dimethylsulfoxide (DMSO)AR1182-A
0.1M EDTA solution11mL (100X)EDTA solution for optional metalloprotease inhibitionAR1182-B

Overview

Product Name Broad Spectrum Protease Inhibitor Cocktail
SKU/Catalog Number AR1182
Physical State Liquid, colorless, transparent
Pack Size 1mLX2
Safety Precautions Harmful. DMSO is toxic and causes irritation to the eyes and skin. Please operate with caution and wear eye and hand protection and proper lab garments.
Recommended working concentration 100-fold dilution in lysis buffer
10 μL of the Protease Inhibitor Cocktail solution is enough to inhibit degradation of proteins in 1 mL lysate.
Storage Upon receipt store at -20°C. It is stable for one year. Product is shipped on ice.
Equivalent Thermofisher (Product No.78430, A32963)

Notes

Type of DAPIContentCatalog Number
Broad Spectrum Protease Inhibitor CocktailBroad Spectrum Protease Inhibitor Cocktail, 1mL (100X)
0.1M EDTA solution, 1mL (100X)
AR1182
Broad Spectrum Protease Inhibitor Cocktail (EDTA free)Broad Spectrum Protease Inhibitor Cocktail, 1mL (100X)AR1182-1

BioChemical Information

Protease Inhibitor Component MW Protease Family Targeted Inhibition Type Typical Working (1X) Conc.
E-64 357.4 Cysteine proteases (papain, calpain, lysosomal cathepsins) Irreversible 15μM
AEBSF 239.5 Serine proteases (trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, kallikrein) Irreversible 1mM
Bestatin 308.4 Amino-peptidases Reversible 50μM
Leupeptin 475.6 Serine and cysteine proteases Reversible 20μM
Aprotinin 6511.5 Serine proteases (trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, kallikrein) Reversible 800nM
Pepstatin A 685.9 Aspartic acid proteases (pepsin and rennin) Reversible 10μM
EDTA 372.2 Metalloproteases (thermolysin and carboxypeptidase A) Reversible 1mM

Assay Principle

Broad Spectrum Protease Inhibitor Cocktail (100X) is a Western blot related ready-to-use concentrated stock solution reagent containing a blend of seven protease inhibitors that is to be added to cell lysis buffer to protect the integrity and functionality of native cellular proteins against degradation by multiple classes of endogenous proteases during protein extraction and sample preparation procedures. The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets.

Properties

Compatibility with reagents Fully compatible with cell lysis buffers and Broad Spectrum Phosphatase Inhibitor Cocktail
Compatibility with assays Not MS-compatible: contain AEBSF;
Not compatible with immobilized metal chelate affinity chromatography and 2D gel electrophoresis: contain EDTA
Reagent Type Western Blotting related reagent; Inhibitors
Usage Protect native cellular proteins from destructive degradation by endogenous proteases following cell lysis
Preserve native cellular proteins intact and functional
Screen extracts for proteolytic activity
Study proteolysis in the regulation of cellular processes
Target Specificity Serine proteases, cysteine proteases, aspartic acid proteases, metalloproteases, aminopeptidases
Target Sample Cell lysis extracts
Description Boster’s Broad Spectrum Protease Inhibitor Cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.
Cite This Product Broad Spectrum Protease Inhibitor Cocktail (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1182)
Application Western blotting, protein purification, Co-IP; assays for protein expression, activity, modification, profiling and characterization, quantitative measurement; epitope tagging; reporter gene analysis
*Our Boster Guarantee covers the use of this product in the above tested applications.

Background

Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. An optimized method to improve the yield of intact and functional native proteins is to add inhibitors of these enzymes known to be present in the source material. This broad spectrum protease inhibitor cocktail is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.
Protease inhibitors are critical reagents for the preservation of protein integrity during purification and analysis procedures by knocking out specific proteases to avoid peptide bond hydrolysis and subsequent protein destruction. They are biological or chemical compounds that function by reversibly or irreversibly binding to the protease. Most known proteases belong to one of four evolutionarily distinct enzyme families based on the functional groups involved in the peptide bond cleavage. No single chemical is effective for all types of proteases. A mixture or "cocktail" of several different inhibitor compounds is commonly used that consistently inhibit a multitude of protease classes to ensure that protein extracts do not degrade before analysis for target proteins of interest. Not using protease inhibitors leads to the loss of a large number of valuable proteins in a lysate sample, adversely affecting downstream applications by biologically meaningless representation of protein activities and gaining false negative immunostaining results for targets of interest.

Usage and Handling

Notes:

• Equilibrate the bottle to room temperature before use.

• Vortex the bottle before use to ensure a homogeneous suspension

• This protease inhibitor cocktail is supplied at a 100X concentration in DMSO and is generally effective when used at a 1X final concentration; however, if a sample contains particularly high levels of proteases, the effective cocktail concentration might require optimization.

1. Add broad spectrum protease inhibitor cocktail in lysis buffer at a ratio of 1:100 and mix well.

2. Optional: For inhibition of metalloproteases, add 0.1M EDTA solution in lysis buffer at a ratio of 1:100 and mix well.

3. Add the solution in to cell or tissue samples for protein extraction.
Lysis buffer containing protease inhibitor cocktail should be freshly prepared before use.

Validation Images & Assay Conditions

AR1182 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Apolipoprotein E4 Impairs in vivo Hippocampal Long-Term Synaptic Plasticity by Reducing the Phosphorylation of CaMKIIα and CREB

Overendocytosis of superparamagnetic iron oxide particles increases apoptosis and triggers autophagic cell death in human osteosarcoma cell under a spinning magnetic field

The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk and NF-κB

Functional Role of NRF2 in Cervical Carcinogenesis

Spinal 5-HT3 receptor contributes to somatic hyperalgesia induced by sub-chronic stress:

UNC5B Promotes Vascular Endothelial Cell Senescence via the ROS-Mediated P53 Pathway

SFKs/p38 Pathway is Involved in Radicular Pain by Promoting Spinal Expression of Pro-Inflammatory Cytokines in a Rat Model of Lumbar Disc Herniation

Capsaicin enhances the antitumor activity of sorafenib in hepatocellular carcinoma cells and mouse xenograft tumors through increased ERK signaling

Puerarin alleviate radicular pain from lumbar disc herniation by inhibiting ERK-dependent spinal microglia activation

Hydrogen sulfide reduces pyroptosis and alleviates ischemia-reperfusion acute kidney injury by inhibiting NLRP3 inflammasome

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2 Customer Q&As for Broad Spectrum Protease Inhibitor Cocktail (100x)

Question

Does AR1182 contain proteins or peptides?

Verified customer

Asked: 2019-07-01

Answer

The Broad Spectrum Protease Inhibitor Cocktail (100x) (AR1182) doesn't contain any proteins or peptides. It is composed of chemicals.

Boster Scientific Support

Answered: 2019-07-02

Question

Are there any proteins or peptides in this product?

Verified Customer

Verified customer

Asked: 2018-07-17

Answer

AR1182 does not contain any proteins or peptides.

Boster Scientific Support

Answered: 2018-07-17

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