Caspase-4 Activity Assay Kit

SKU AR4006
Application Enzyme Activity
Detection Method Colorimetric
Sample type Tissue Extracts, Cell Lysate
Storage -20°C

Product Info Summary

SKU: AR4006
Size: From 50 Assays to 100 Assays
Application: Enzyme Activity

Introduction

Caspase-4 is a member of the cysteine-aspartic acid protease family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Our Caspase-4 Assay Kit, Colorimetric provides a simple and convenient method for assaying the activity of Caspase-4, which is based on the hydrolysis of the labeled substrate Ac-LEVD-pNA (acetyl-Leu-Glu-Val- Asp p-nitroanilide) by Caspase-4, releasing the pNA (p-nitroaniline) moiety from the substrate. The released pNA has the max absorbance at 405 nm (εmM = 10.5) and its concentration is calculated by measuring the absorbance values at 405 nm or from a standard calibration curve prepared using the pNA Dye Standard, using a microplate reader or spectrophotometer.

The assay can be performed in 100 µl volume in a 96 well plate using an ELISA plate reader or in 1 ml volume and measured in a spectrophotometer, using quartz cuvettes, since plastic cuvettes attenuate the absorption at 405 nm. Comparison of the absorbance of the released pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-4 activity.

Overview

Product Name Caspase-4 Activity Assay Kit
SKU/Catalog Number AR4006
Description Our Caspase-4 Assay Kit, Colorimetric provides a simple and convenient method for assaying the activity of Caspase-4, which is based on the hydrolysis of the labeled substrate Ac-LEVD-pNA (acetyl-Leu-Glu-Val- Asp p-nitroanilide) by Caspase-4, releasing the pNA (p-nitroaniline) moiety from the substrate. The released pNA has the max absorbance at 405 nm (εmM = 10.5) and its concentration is calculated by measuring the absorbance values at 405 nm or from a standard calibration curve prepared using the pNA Dye Standard, using a microplate reader or spectrophotometer.
Cite This Product Caspase-4 Activity Assay Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR4006)

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SKU:AR4006

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