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Product Info Summary
| SKU: | BA1060 |
|---|---|
| Size: | 0.5ml |
| Reactive Species: | Goat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Overview
| Product Name | HRP Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L) |
|---|---|
| Synonyms | HRP-conjugated rabbit anti-goat IgG |
| Description | HRP Conjugated Rabbit Anti-goat IgG (H+L) (gamma-chain specific) secondary antibody This HRP conjugated antibody is specific for goat IgG, has weak cross-reactivity with human IgG, and shows no cross-reactivity with rat/ mouse IgG. |
| Reagent Type | Secondary antibody, reporter enzyme labeled |
| Label | HRP (Horseradish Peroxidase) |
| Host | Rabbit |
| Target Species | Goat |
| Antibody Class | IgG |
| Clonality | Polyclonal |
| Immunogen | Goat IgG (whole molecular) |
| Purification | The antibody was purified from antisera by immunoaffinity chromatography. |
| Specificity | This HRP conjugated rabbit anti-goat antibody has weak cross-reactivity with human IgG and shows no cross-reactivity with rat/mouse IgG. |
| Form Supplied | Concentrated, Liquid |
| Formulation | 0.5 mg of HRP conjugated specific antibody
0.01 M PBS (PH 7.4) 50% glycerol |
| Pack Size | 0.5ml |
| Concentration | 1mg/ml |
| Application | ELISA*, WB* *Our Boster Guarantee covers the use of this product in the above marked tested applications. |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Precautions | FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE |
Assay Information
| Sample Type | SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates |
|---|---|
| Assay Type | Immunoassay |
| Assay Purpose | Protein detection/quantification |
| Technique | Immunodetection of target antibody with HRP reporter enzyme |
| Equipment Needed | WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer |
| Compatibility with Reagents | Incompatible with sodium azide and metals incompatible with high phosphate concentrations |
Main Advantages
| Specific | High signal-to-noise ratio |
|---|---|
| Sensitive | Detects low-abundant targets due to an optimal number of HRP molecules per antibody |
| High Signal Amplification | Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC |
| Fast | Generates strong signals in a relatively short time span |
| Quantifieable | Allows quantification of detected signal |
| Easy to Use | Supplied in a workable liquid format |
| Flexible | HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates; |
| Convenient | HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes |
Background
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.
Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) Direct ELISA: 0.05-0.5μg/ml (TMB detection)
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For BA1060
Hello CJ!
BA1060 has been cited in 164 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model
Atorvastatin ameliorates myocardial ischemia/reperfusion injury through attenuation of endoplasmic reticulum stress-induced apoptosis
Integrated mRNA and miRNA profiling in NIH/3T3 cells in response to bovine papillomavirus E6 gene expression
Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression
Effect of Resuscitation Fluids on the Expression of hsp90α in Cardiac Muscles of Hemorrhagic Shocked Rats
miR106b regulates retinoblastoma Y79 cells through Runx3
Overexpression of TPX2 is associated with progression and prognosis of prostate cancer
Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes
OCT3/4 expression is correlated with the invasion of gastric carcinoma
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1 Customer Q&As for HRP Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)
Question
The customer wanted the CoA for the current lot of the 100mg of BA1060-1 we have in stock . Could you provide the CoA with all the required testing information? The customer is requesting prices for the following : 10 x 1mg test vials 90 x 1mg bulk Do you have a suggested price?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
Our current new lot has around 10% cross reactivity with human IgG. Please see attached CoA of the current new lot# BST14E08A60. This new lot has weak cross reactivity with human IgG. Please see the following suggested price 10 x 1mg test vials 40% off 90 x 1mg bulk 50% off Please let me know if you have any other questions.
Boster Scientific Support
Answered: 2018-11-22
