Product Info Summary
SKU: | EK2139 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human ACP6 ELISA Kit PicoKine®
SKU/Catalog Number
EK2139
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ACP6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACP6 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ACP6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2139)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: E33-E428
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2139 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2139 is reactive to ACP6 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2139 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ACP6
Lysophosphatidic acid phosphatase type 6 is an acid phosphatase enzyme that is encoded in humans by the ACP6 gene. This gene encodes a member of the histidine acid phosphatase protein family. The encoded protein hydrolyzes lysophosphatidic acid, which is involved in G protein-coupled receptor signaling, lipid raft modulation, and in balancing lipid composition within the cell. Alternative splicing results in multiple transcript variants.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2139-CAP | Anti-Human ACP6 Pre-coated 96-well strip microplate | 1 |
EK2139-ST | Human ACP6 Standard | 2 vials, 10 ng/tube |
EK2139-DA | Human ACP6 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
O.D. | 0.028 | 0.061 | 0.133 | 0.243 | 0.465 | 0.856 | 1.242 | 2.365 |
Data Example Images
Click image to see more details
Human ACP6 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ACP6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 25 | 166 | 457 | 29 | 163 | 414 |
Standard deviation | 1.4 | 9.46 | 24.22 | 1.04 | 7.99 | 18.63 |
CV(%) | 5.6% | 5.7% | 5.3% | 3.6% | 4.9% | 4.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ACP6 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 25 | 28 | 29 | 24 | 27 | 0.95 | 3.6% |
Sample 2 | 166 | 157 | 169 | 174 | 167 | 4.83 | 2.9% |
Sample 3 | 457 | 479 | 485 | 460 | 470 | 14.58 | 3.1% |
Protein Target Info & Infographic
Gene/Protein Information For ACP6 (Source: Uniprot.Org, NCBI)
Gene Name
ACP6
Full Name
Lysophosphatidic acid phosphatase type 6
Weight
48.886kDa
Superfamily
histidine acid phosphatase family
Alternative Names
acid phosphatase 6, lysophosphatidicPACPL1; Acid phosphatase-like protein 1; ACP6; ACPL1; ACPL1acid phosphatase like 1; EC 3.1.3.2; LPAP; LPAPlysophosphatidic acid phosphatase type 6; PACPL1 ACP6 ACPL1, LPAP, PACPL1 acid phosphatase 6, lysophosphatidic lysophosphatidic acid phosphatase type 6|acid phosphatase-like protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ACP6, check out the ACP6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACP6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ACP6 ELISA Kit PicoKine® (EK2139)
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