Human AFP/Alpha-fetoprotein ELISA Kit PicoKine®

alpha-Fetoprotein/AFP ELISA kit for Human

Human AFP/Alpha-fetoprotein ELISA Kit PicoKine™ (96 Tests). Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0651
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human AFP/Alpha-fetoprotein ELISA Kit PicoKine®

View all alpha-Fetoprotein/AFP ELISA kits

SKU/Catalog Number

EK0651

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human AFP/Alpha-fetoprotein ELISA Kit PicoKine™ (96 Tests). Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human AFP/Alpha-fetoprotein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0651)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R19-V609

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0651 is reactive to AFP in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0651 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of alpha-Fetoprotein/AFP

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

Kit Components

Catalog Number Description Quantity
EK0651-CAP Anti-Human AFP Pre-coated 96-well strip microplate 1
EK0651-ST Human AFP Standard 2 vials, 5 ng/tube
EK0651-DA Human AFP Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0030.1370.2480.4860.7771.2601.7041.925

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect alpha-Fetoprotein/AFP, Dilution ratio of 1:5, concentration in serum is around 5ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human AFP/Alpha-fetoprotein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)986432190986541986
Standard deviation4.5647.58100.745.5856.24129.09
CV (%)4.8%7.4%4.6%5.7%8.6%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of alpha-Fetoprotein/AFP in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1951051091071045.385.1%
Sample 264373268271069133.254.8%
Sample 32190229024202404232693.154%
*number of samples for each test n=16.

Gene/Protein Information For AFP (Source: Uniprot.Org, NCBI)

Uniprot ID

P02771

Gene ID

174

Gene Name

AFP

Full Name

Alpha-fetoprotein

Weight

68.678kDa

Superfamily

ALB/AFP/VDB family

Alternative Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AFP, check out the AFP Infographic

AFP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AFP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0651 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Rapid analysis of alpha-fetoprotein by chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads

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6 Customer Q&As for Human AFP/Alpha-fetoprotein ELISA Kit PicoKine®

Question

Q: Can alpha-Fetoprotein ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-11-01

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-11-01

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

A. Jones

Verified customer

Asked: 2019-10-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-10-19

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification alpha-Fetoprotein using the EK0651 Human alpha-Fetoprotein Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-09-24

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-01-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-01-11

Question

Q: is it okay to use citrate plasma as samples in Human alpha-Fetoprotein Picokine® ELISA Kit (Catalog # EK0651)?

Verified Customer

Verified customer

Asked: 2017-09-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of alpha-Fetoprotein causing degradation of its protein structure. alpha-Fetoprotein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the alpha-Fetoprotein ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-09-15

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of alpha-Fetoprotein in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

C. Sunder

Verified customer

Asked: 2016-06-08

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the alpha-Fetoprotein ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-06-08

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