Human BMP-5 ELISA Kit PicoKine™

Human BMP-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BMP5 in cell culture supernatants, serum and bone tissue. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0310
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and bone tissue.

Product Name

Human BMP-5 ELISA Kit PicoKine™

See all BMP-5 products

SKU/Catalog Number

EK0310

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human BMP-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BMP5 in cell culture supernatants, serum and bone tissue. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human BMP-5 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0310)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A317-H454

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0310 is reactive to BMP5 in Human samples

Validated Sample Types

cell culture supernatants, serum and bone tissue.

Application Guarantee

EK0310 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0310-CAP Anti-Human BMP5 Pre-coated 96-well strip microplate 1
EK0310-ST Human BMP5 Standard 2 vials, 10 ng/tube
EK0310-DA Human BMP5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0030.1010.1430.2750.4370.8891.5212.526

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect BMP-5, Dilution ratio of 1:1, concentration in serum is around 60pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human BMP-5 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2421573436326616834290
Standard deviation16.4569.21314.1320.7482.46347.49
CV (%)6.8%4.4%7.2%7.8%4.9%8.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BMP-5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12422282462392386.682.8%
Sample 215731794186315901705126.027.3%
Sample 343634914415744954482276.846.1%
*number of samples for each test n=16.

Gene/Protein Information For BMP5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22003

Gene ID

653

Gene Name

BMP5

Full Name

Bone morphogenetic protein 5

Weight

51.737kDa

Superfamily

TGF-beta family

Alternative Names

BMP5; BMP-5; bone morphogenetic protein 5; MGC34244 BMP5 bone morphogenetic protein 5 bone morphogenetic protein 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMP5, check out the BMP5 Infographic

BMP5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BMP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human BMP-5 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for BMP-5 ELISA kit? I performed your BMP-5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BMP-5 even though the O.D. values are not very high?

D. Liu

Verified customer

Asked: 2020-08-20

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP-5 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of BMP-5 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-08-20

Question

Q: if the enzyme conjugated BMP-5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP-5 antigen?

Verified Customer

Verified customer

Asked: 2019-03-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-03-12

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2017-02-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-02-19

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

R. Puri

Verified customer

Asked: 2016-01-26

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-01-26

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis BMP-5 using the EK0310 Human BMP-5 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2015-02-06

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2015-02-06

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