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Product Info Summary
| SKU: | EK0563 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine®
View all Cardiotrophin-1/CT-1 ELISA kits
SKU/Catalog Number
EK0563
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0563)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S2-A201
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0563 is reactive to CTF1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0563 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cardiotrophin-1/CT-1
Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0563-CAP | Anti-Human CTF1 Pre-coated 96-well strip microplate | 1 |
| EK0563-ST | Human CTF1 Standard | 2 vials, 10 ng/tube |
| EK0563-DA | Human CTF1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.031 | 0.059 | 0.095 | 0.139 | 0.277 | 0.534 | 1.288 | 2.278 |
Data Example Images
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Human Cardiotrophin-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cardiotrophin-1/CT-1, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 253 | 924 | 3655 | 274 | 930 | 3410 |
| Standard deviation | 12.65 | 69.3 | 288.74 | 16.16 | 70.68 | 300.08 |
| CV (%) | 5% | 7.5% | 7.9% | 5.9% | 7.6% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cardiotrophin-1/CT-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 253 | 241 | 250 | 241 | 246 | 5.35 | 2.1% |
| Sample 2 | 924 | 988 | 933 | 938 | 945 | 24.9 | 2.6% |
| Sample 3 | 3655 | 4348 | 4273 | 3862 | 4034 | 286.76 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For CTF1 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q16619
Gene ID
1489
Gene Name
CTF1
Full Name
Cardiotrophin-1
Weight
21.227kDa
Superfamily
IL-6 superfamily
Alternative Names
cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1 CTF1 CT-1, CT1 cardiotrophin 1 cardiotrophin-1|cardiophin 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CTF1, check out the CTF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine® (EK0563)
No publications found for EK0563
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
11 Customer Q&As for Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured Cardiotrophin-1 level in serum.
B. Thomas
Verified customer
Asked: 2020-11-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-11-18
Question
Q: Can Cardiotrophin-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-10-31
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-31
Question
Q: can I use citrate plasma as samples in Human Cardiotrophin-1 Picokine® ELISA Kit (Catalog # EK0563)?
G. Wright
Verified customer
Asked: 2020-10-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Cardiotrophin-1 causing disruption of its protein structure. Cardiotrophin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Cardiotrophin-1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-10
Question
Q: if the enzyme conjugated Cardiotrophin-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cardiotrophin-1 antigen?
P. Green
Verified customer
Asked: 2020-03-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-05
Question
Q: What is the optimal O.D. value for Cardiotrophin-1 ELISA kit? I performed your Cardiotrophin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Cardiotrophin-1 even though the O.D. values are not very high?
H. Hernandez
Verified customer
Asked: 2019-12-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cardiotrophin-1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Cardiotrophin-1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-12-27
Question
In the EK0563 manual provided it is stated to store all reagents at -20°C. However, on the label of the reagents/plates/standards it is stated to store them at 4°C (as we did). Could this difference in storage temperature be of influence on cardiotrophin that is measured in the samples?
Verified customer
Asked: 2019-04-09
Answer
There should not be any storage condition on the label of the reagents/plates/standards for the Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine™ (EK0563). We only state the storage condition on the label sticking outside the kit box and in the manual. "Store at 4°C for 6 months, at -20°C for 12 months." So, since you are storing the product components at these temperatures, there should not be an influence on the performance.
Boster Scientific Support
Answered: 2019-04-10
Question
As stated the EK0563 kit is able to measure Cardiotrophin in EDTA plasma what was the concentration you measured?
Verified customer
Asked: 2019-04-09
Answer
The concentration range of Cardiotrophin in EDTA plasma for the Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine™ (EK0563) is 230-650pg/mL.
Boster Scientific Support
Answered: 2019-04-10
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-02-09
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-02-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Campbell
Verified customer
Asked: 2019-01-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-24
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Cardiotrophin-1 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cardiotrophin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-01-01
