Human CFHR5 ELISA Kit PicoKine®

Complement Factor H-related 5/CFHR5 ELISA kit for Human

Human CFHR5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 75 pg/ml.

Product Info Summary

SKU: EK1933
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Product Name

Human CFHR5 ELISA Kit PicoKine®

View all Complement Factor H-related 5/CFHR5 ELISA kits

SKU/Catalog Number

EK1933

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CFHR5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 75 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CFHR5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1933)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-E569

Sensitivity

<75 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1933 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1933 is reactive to CFHR5 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Application Guarantee

EK1933 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Complement Factor H-related 5/CFHR5

Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).

Kit Components

Catalog Number Description Quantity
EK1933-CAP Anti-Human CFHR5 Pre-coated 96-well strip microplate 1
EK1933-ST Human CFHR5 Standard 2 vials, 50 ng/tube
EK1933-DA Human CFHR5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01563126251,2502,5005,00010,000
O.D.0.0240.1210.1630.2890.6220.9061.5732.081

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Complement Factor H-related 5/CFHR5, Dilution ratio of 1:1000, concentration in serum and plasma is 1.6-3.1 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CFHR5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)19043225442945104693
Standard deviation12.2418.14170.1912.9436.77334.61
CV (%)6.4%4.2%6.7%4.4%7.2%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Factor H-related 5/CFHR5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12302231993032398.693.6%
Sample 232162358942749038.517.9%
Sample 328474318308825453200167.975.3%
*number of samples for each test n=16.

Gene/Protein Information For CFHR5 (Source: Uniprot.Org, NCBI)

Gene Name

CFHR5

Full Name

Complement factor H-related protein 5

Weight

64.419kDa

Alternative Names

CFHL5; CFHL5factor H-related protein 5; CFHR5; Complement Factor Hrelated 5; Complement Factor H-related 5; FHR5; FHR-5complement factor H-related protein 5; FHR5MGC133240; FLJ10549 CFHR5 CFHL5D, FHR-5, FHR5, CFHR5 complement factor H related 5 complement factor H-related protein 5|factor H-related protein 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CFHR5, check out the CFHR5 Infographic

CFHR5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CFHR5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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9 Customer Q&As for Human CFHR5 ELISA Kit PicoKine®

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CFHR5 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

M. Dhar

Verified customer

Asked: 2019-10-04

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CFHR5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-10-04

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-08-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-17

Question

Q: which procedure should I follow in order to thaw whole blood sample for CFHR5 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CFHR5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-07-01

Question

Q: What is the optimal O.D. value for CFHR5 ELISA kit? I used your CFHR5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CFHR5 even though the O.D. values are not very high?

B. Dhawan

Verified customer

Asked: 2019-01-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CFHR5 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of CFHR5 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.

Boster Scientific Support

Answered: 2019-01-02

Question

Q: if the enzyme conjugated CFHR5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CFHR5 antigen?

Verified Customer

Verified customer

Asked: 2018-09-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-09-16

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-09-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-09-04

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-11-07

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-11-07

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

W. Wright

Verified customer

Asked: 2017-06-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-06-29

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CFHR5 using the EK1933 Human CFHR5 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-02-28

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2017-02-28

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