Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

Chitotriosidase/CHIT1 ELISA kit for Human

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHIT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2032
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

View all Chitotriosidase/CHIT1 ELISA kits

SKU/Catalog Number

EK2032

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHIT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2032)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-N466

Sensitivity

<50 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2032 is reactive to CHIT1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2032 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Chitotriosidase/CHIT1

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1q32.1. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.

Kit Components

Catalog Number Description Quantity
EK2032-CAP Anti-Human CHIT1 Pre-coated 96-well strip microplate 1
EK2032-ST Human CHIT1 Standard 2 vials, 20 ng/tube
EK2032-DA Human CHIT1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0190.1490.2660.5011.1161.3551.7872.022

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Chitotriosidase/CHIT1, Dilution ratio of 1:10, concentration in serum is around 20ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Chitotriosidase/CHIT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5901670793359419588471
Standard deviation30.6871.81325.2539.20113.56559.09
CV (%)5.2%4.3%4.1%6.6%5.8%6.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Chitotriosidase/CHIT1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 159057261069061545.067.3%
Sample 216701703194117711771104.555.9%
Sample 379338323909877788283510.726.1%
*number of samples for each test n=16.

Gene/Protein Information For CHIT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q13231

Gene ID

1118

Gene Name

CHIT1

Full Name

Chitotriosidase-1

Weight

51.681kDa

Superfamily

glycosyl hydrolase 18 family

Alternative Names

CHI3; CHIT1; CHITCHI3; chitinase 1 (chitotriosidase); chitinase-1; Chitotriosidase; chitotriosidase-1; EC 3.2.1.14; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase CHIT1 CHI3, CHIT, CHITD chitinase 1 chitotriosidase-1|chitinase 1 (chitotriosidase)|plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHIT1, check out the CHIT1 Infographic

CHIT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHIT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CHIT1 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

S. Zhao

Verified customer

Asked: 2020-11-13

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CHIT1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-11-13

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CHIT1 using the EK2032 Human CHIT1 Picokine® ELISA Kit?

L. Lee

Verified customer

Asked: 2019-03-16

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-03-16

Question

Q: which procedure should I follow in order to thaw whole blood sample for CHIT1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-01-16

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CHIT1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-01-16

Question

Q: if the enzyme conjugated CHIT1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CHIT1 antigen?

Verified Customer

Verified customer

Asked: 2017-11-21

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-11-21

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